The Correlation of Endothelial Nitric Oxide Synthase (eNOS) Polymorphism and Other Risk Factors with Aneurysmal Subarachnoid Hemorrhage: A Case-Control Study

Konar, Subhas; Ramesh, Shruthi; Christopher, Rita; Prasanthi, Aripirala; Bhat, Dhananjaya I; Shukla, Dhaval; Bharath, R D; Devi, Bhagavatula Indira

doi:10.4103/0028-3886.266231

Cited by 3 publications

(1 citation statement)

References 31 publications

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“…eNOS is an enzyme responsible for the production of nitric oxide (NO), which is a potent vasodilator. 28 We observed that eNOS expression was higher in both the SAH (control) group and the SAH+PcTx1 group compared to the sham group. This suggests a compensatory response to the vascular changes induced by SAH, where the endothelium attempts to counteract vasospasm by increasing NO production.…”

Section: Discussionmentioning

confidence: 71%

Pctx1 venom in the treatment of vasospasm due to experimental subarachnoidal hemorrhage

AKGÜN,

AKGÜL

2023

J Health Sci Med / JHSM

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Aims: We aimed to investigate the role of neuron damage in experimental animals following vasospasm, by increasing perfusion of neuronal tissue through vasodilation using the venom of PcTx1, and to determine its effectiveness in reducing neuron damage after vasospasm. Methods: Thirty adult male Wistar albino rats weighing between 300 and 400 grams were used and divided into three groups: the Sham group (Group 1, n=10), to which no application was made; the SAH (control) group (Group 2, n=10), in which a double SAH model was created and 1 cc of saline was administered intraperitoneally; and the SAH+PcTx1 group (Group 3, n=10), in which a double SAH model was created and 1 cc/kg of PcTx1 venom was administered intraperitoneally daily. Basilar artery diameter and immunochemical measurements were performed histopathologically, and neurohistopathological findings were scored semiquantitatively in terms of vascular changes, neuron degeneration, gliosis, and bleeding criteria using a scale of 0 (none), 1 (mild), 2 (moderate), or 3 (severe). eNOS immunopositivity was also evaluated. The detection of apoptosis in the brain was performed by evaluating the effector enzyme caspase-3 immunoreactivity of the exogenous apoptosis pathway. Results: The most severe vascular spasm and degeneration-necrosis of brain tissue gray matter neurons were seen in Group 2, whereas the vascular narrowing was less severe in Group 3. Brain parenchyma and neuron and neuroglial reactions were milder in Group 3. eNOS expression was detected at a higher level in Group 1, Group 2, and Group 3, respectively. For apoptosis and caspase-3 immunoreactivity of the exogenous apoptosis pathway, no immunopositive reactions were observed in Group 1. Conclusion: For the occurrence and formation mechanisms of vasospasm after subarachnoid hemorrhage, this pathological condition is thought to result from multifactorial and various biochemical reactions. In our study, it was found that psalmotoxin effectively prevented vasospasm and significantly reduced tissue damage after vasospasm.

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Section: Discussionmentioning

confidence: 71%

Pctx1 venom in the treatment of vasospasm due to experimental subarachnoidal hemorrhage

AKGÜN,

AKGÜL

2023

J Health Sci Med / JHSM

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Investigation of eNOS G894T Gene Polymorphism in Patients with Pseudoexfoliation Syndrome: A preliminary Study

Özer¹

2022

Beyoglu Eye J

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The Effect of Nos3 and Agtr1 Genotypes on the Course of the Arterial Hypertension for the Overweight or Obese Patients

et al. 2022

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The aim: Objective of the research is to determine the effect of NOS3 and AGTR1 genotypes of patients with arterial hypertension and high body mass index in the course of the disease. Materials and methods: 58 patients (22 men and 36 women) with AH and high BMI were examined. The average age of the examined patients was 53.6±8.7 years. The analysis of rs1799983 polymorphisms of the NOS3 gene (localization 7q36.1; 7:150999023) and AGTR1 (type 1 receptor for angiotensin 2 1166 A>C) was performed using TaqMan assay (Thermo Fisher Scientific, USA) by real-time PCR (Applied Biosystems, USA) using TaqMan probe amplification products. Genomic DNA samples were isolated from stabilized blood using a Genomic DNA Mini Kit reagent (Invitrogen, USA). The Statistica 10 program (StatSoft Inc.) was used for statistical processing of the obtained data, USA). The independent samples were compared using the Mann-Whitney (U) criterion. In all cases of statistical evaluation, the reliability of differences was taken into account at a value of p<0.05. Results and conclusions: Polymorphism of the NOS3 and AGTR1 genes is associated with early development and complicated course of cardiovascular pathology. The combination of NOS3 and AGTR1 gene polymorphism in patients with the high body mass index increases the risk of complications in hypertension. Using a mathematical model to predict the probability (95%) of genetic mutations in two genes (NOS3 and AGTR1) increases the effectiveness of diagnosis for patients with the high risk of developing cardiovascular complications.

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The Correlation of Endothelial Nitric Oxide Synthase (eNOS) Polymorphism and Other Risk Factors with Aneurysmal Subarachnoid Hemorrhage: A Case-Control Study

Cited by 3 publications

References 31 publications

Pctx1 venom in the treatment of vasospasm due to experimental subarachnoidal hemorrhage

Pctx1 venom in the treatment of vasospasm due to experimental subarachnoidal hemorrhage

Investigation of eNOS G894T Gene Polymorphism in Patients with Pseudoexfoliation Syndrome: A preliminary Study

The Effect of Nos3 and Agtr1 Genotypes on the Course of the Arterial Hypertension for the Overweight or Obese Patients

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