The correlation between preputial blood flow and microvessel density in distal hypospadias: A prospective clinical study

Ceyhan, Levent; Savaş, Mustafa Çağrı; Başpınar, Şirin; Duman, Levent; Büyükyavuz, Behçet İlker

doi:10.1016/j.jpurol.2013.07.003

Cited by 6 publications

(9 citation statements)

References 15 publications

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“…The prepuce vessels are the terminal branch, and the capillaries are extremely narrow, hence knowledge about the vessels is limited. Considerable work has been done to study prepuce vessels, such as transillumination with endoscopic cold light source, microscopic observation of gelation and India ink perfusion, 3D reconstruction of histological sections [10][11][12]. In this study, an advanced technology, micro-CT scanning, was used to analyze the distribution of preputial vessels.…”

Section: Discussionmentioning

confidence: 99%

“…So far little research has been carried out in the study of preputial vascular distribution of patients with hypospadias. Previous literature has reviewed the anatomy of hypospadias preputial vessels using methods such as trans-illumination, microscopic observation and 3D computer reconstruction [10][11][12]. Although these approaches have studied the vascular anatomy, the preputial vascular networks are still difficult to measure in 3D.…”

Section: Introductionmentioning

confidence: 99%

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The Distribution of Preputial Vessels in Different Severity of Rat Congenital Hypospadias Model: Imaging study using Micro-Computerized Tomograph

Lin

Liu

Wang

et al. 2019

Preprint

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Background: Micro-computerized tomography (micro-CT) is considered as an innovative non-invasive and high-resolution imaging technology. The current research aims to reconstruct the distribution of preputial vessels in different severity of rat congenital hypospadias model by micro-CT, and to provide an anatomic basis for the selection of preputial vessel pedicle flaps in surgery. Methods: Pregnant rats were exposed to finasteride from gestational day 12 to 17. Depending on the position of the urethral meatus, the pups were divided into normal, mild hypospadias and severe hypospadias groups. Six months after birth, the preputial blood vessels were observed in vascular perfusion with Microfil (a silicone-based polymer) and scanned by micro-CT. CTvox and NRecon were utilized to reconstruct 3-dimentional(3D) images. A pathological analysis of the specimen was taken in order to determine the position of Microfil. Results: The normal group and the mild hypospadias group had similar preputial image characteristics. At the junction of the inner and outer prepuce, the deep layer vessels of the superficial fascia were transversely distributed and formed a vascular ring-like structure. Among the severe hypospadias group, five had sufficient blood circulation while six had insufficient blood circulation. In sufficient blood circulation type, the ring-like vessels were found at the junction of the inner and outer prepuce similar to that of the normal and mild hypospadias group. However, only a small amount of capillary supply to this area in the insufficient type. Conclusion: The junction of the inner and outer prepuce with abundant blood circulation was suitable to be a vascular pedicle flap. The tubularized preputial island flaps were consistent with the ring-like vessels area, and the original blood supply was retained to the greatest extent.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Distribution of Preputial Vessels in Different Severity of Rat Congenital Hypospadias Model: Imaging study using Micro-Computerized Tomograph

Lin

Liu

Wang

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…The prepuce vessels are the terminal branch, the capillaries are extremely narrow, thus knowledge about the vessels is limited. Much work has been done to study prepuce vessels, such as transillumination with endoscopic cold light source, microscopic observation of gelation and India ink perfusion, 3D reconstruction of histological sections [10][11][12]. In this study, an advanced technology, micro-CT scanning, is used to analysis the distribution of preputial vessels.…”

Section: Discussionmentioning

confidence: 99%

“…So far little research has been done in the study of preputial vascular distribution of patients with hypospadias. Several literatures have described the anatomy of hypospadias preputial vessels using methods such as trans-illumination, microscopic observation and 3D computer reconstruction [10][11][12]. Although these approaches have described the vascular anatomy, the preputial vascular networks are still difficult to quantify in 3D.…”

Section: Introductionmentioning

confidence: 99%

The Distribution of Preputial Vessels at Different Severity of Rat Congenital Hypospadias Model

Lin

Liu

Wang

et al. 2019

Preprint

View full text Add to dashboard Cite

Background To reconstruct and analyze the distribution of preputial vessels using micro-computerized tomography (micro-CT) in the finasteride induced congenital hypospadias rat model. Materials and methods Pregnant rats were exposed to finasteride from gestational days 12 to 17. According to the position of the urethral meatus, the pups were divided into normal, mild hypospadias and severe hypospadias groups. Six months after birth, the preputial blood vessels were observed by vascular perfusion with Microfil (a silicone-based polymer) and scanned by micro-CT. CTvox and NRecon were used to reconstruct 3-dimentional(3D) images. A pathological analysis of the specimen was taken to determine the position of Microfil. Results The normal group and the mild hypospadias group had similar preputial image characteristics. At the junction of the inner and outer prepuce, the deep layer vessels of the superficial fascia were transverse distributed and formed a vascular ring-like structure. Among the severe hypospadias group, five had sufficient blood circulation while six had insufficient blood circulation. In the sufficient blood circulation type, the ring-like vessels were found at the junction of the inner and outer prepuce similar to the normal and mild hypospadias group. However, only a small amount of capillary supply to this area in the insufficient type. Conclusion Compares to the traditional techniques, micro-CT improves understanding the 3D structure of the hypospadias preputial vascular. The junction of the inner and outer prepuce with rich blood circulation was suitable to be a vascular pedicle flap.

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“…This finding suggests that blood supply to the neourethra or the occurrence of postoperation complications may be associated with microvessel density. Ceyhan et al (25) studied this possibility, and reported that reduced preputial microvessel density did not influence blood flow in cases of distal hypospadias. However, the relationship between blood flow and preputial microvessel density in other types of hypospadias (e.g., degrees III and IV) has not been investigated.…”

Section: Discussionmentioning

confidence: 99%

Vascularization of vessel pedicle in hypospadias and its relationship to near‑period complications

2018

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The present study evaluated the relationship between the vascularization of vessel pedicle and the near-period complication by assessing the surgery effect and recognizing the blood supply of the material for urethra reconstruction and collected the follow-up of the hypospadias patients after the operation. We illuminated the prepuce by a lighting technique using cold light and then photographed, and recorded the distribution and the quantity of vessels. Then classified, analyzed and summarized the vascularization of vessel pedicle. Patients who were repaired by Duckett technique were followed up for 1–5 months after the operation. We classified vessel pedicle vascularization in hypospadias cases into four patterns based on the predominant blood vessels. The number of predominant vessels had no exact relationship to the meatal location. The incidence of fistula and stricture had no exact relationship to the number of predominant vessels. In conclusion, there is no exact congruent relationship between the types of vascularization with the meatal location. Vascularization of vessel pedicle had no exact relationship with urethral fistula or stricture.

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The correlation between preputial blood flow and microvessel density in distal hypospadias: A prospective clinical study

Cited by 6 publications

References 15 publications

The Distribution of Preputial Vessels in Different Severity of Rat Congenital Hypospadias Model: Imaging study using Micro-Computerized Tomograph

The Distribution of Preputial Vessels in Different Severity of Rat Congenital Hypospadias Model: Imaging study using Micro-Computerized Tomograph

The Distribution of Preputial Vessels at Different Severity of Rat Congenital Hypospadias Model

Vascularization of vessel pedicle in hypospadias and its relationship to near‑period complications

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