The correlation between plasma human neutrophil peptide 1-3 levels and severity of coronary artery disease

Abstract

<b>Introduction: </b> Inflammation plays a key role in atherosclerosis, and discovering new biomarkers of inflammation is becoming important in order to uncover the pathogenesis of atherosclerotic coronary artery disease (CAD). Recent studies have focused on polymorphonuclear neutrophils. It has been suggested that human neutrophil peptide 1-3 (HNP1-3) is proatherogenic. In this study, we aimed to investigate the associations between plasma HNP1-3 levels and the severity of atherosclerosis via a generally accepted scoring system.<br /> <b>Material and methods</b>: This cross-sectional, observational study included 107 consecutive patients suffering from stable angina pectoris and undergoing coronary angiography (CAG). Patients were divided into two groups according to the Gensini scoring (GS) system evaluating disease severity. Group 1 was composed of mild CAD patients with GS < 20 and group 2 consisted of severe CAD patients with GS ≥ 20. Plasma HNP1-3 levels were assessed by the ELISA method.<br /> <b>Results</b>: The mean HNP1-3 levels were found to be lower in group 1 than group 2 (134.7 ng/ml vs. 147.5 ng/ml). HNP1-3 levels were significantly higher in the severe CAD group than the mild CAD group according to GS (p < 0.001). The results of multivariate logistic regression analysis revealed that only age > 62 years and HNP1-3 > 134 ng/ml were independent predictors of the severity of CAD after adjusting for gender, smoking, hypertension, hyperlipidemia, diabetes, family history of CAD and white blood cell count. In predicting the severity of CAD, the sensitivity and specificity of HNP1-3 were 83.9% (p < 0.001) and 58.8% (p < 0.001), respectively.<br /> <b>Conclusions</b>: This study revealed that the plasma levels of HNP1-3 were significantly higher in severe CAD than mild CAD.

Predictive value of baseline alpha defensin level in patients with stable coronary artery disease: A retrospective single center study

“…We also found higher occurrence of ≥ 2 CAD in patients with higher alpha defensin levels, which is in line with previous study showing higher incidence of multiple CAD with higher alpha defensin levels [20] , [34] . Intriguingly, alpha defensin related residual CAD risk was documented despite favorable HbA1c.…”

“…Alpha-defensin showed robust association with both all-cause mortality in heart failure patients [33] , and CAD severity in stable coronary disease patients [20] , [34] . Moreover, alpha-defensin was mechanistically linked with both atherosclerosis [27] and atherothrombosis [28] .…”

Association between tissue human neutrophil peptide 1–3 levels and cardiovascular phenotype: a prospective, longitudinal cohort study

“…In a human cohort of patients with stable angina, plasma HNP1–3 levels were significantly associated with advanced CAD. 22 Paulin et al challenged the pro-atherosclerotic properties of HNP1–3. 23 They claimed a potent atheroprotective effect of HNP ascribed to a reduction in plasma LDL-cholesterol levels by facilitating the clearance of LDL particles in the liver via the LDL receptor.…”

HNP-1 Reverses Hypertensive Left Ventricular Hypertrophy by Inhibiting the NF-кB Signaling Pathway

“…On the one hand, HNP-1 levels have been associated with the severity of coronary atherosclerosis [41], and may increase the risk of thrombosis by altering fibrin shape, structure, and stability [42]. On the other hand, HNP-1 may prevent atherosclerosis progression and decrease plasma lowdensity lipoprotein cholesterol levels [43], decrease atherosclerosis [44], and mitigate arterial thrombosis by inhibiting the aggregation of von Willebrand factor [45]. Whether HNP-1 plays a role in HLVH remains unclear.…”