The CorA family: Structure and function revisited

Niegowski, Damian; Eshaghi, Said

doi:10.1007/s00018-007-7174-z

Cited by 71 publications

(67 citation statements)

References 53 publications

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“…They seem to be one of the hallmarks of the Mg 2þ transporters subgroup A. However, the residues involved in the metal bindings are not conserved in the CorA subgroup B, 21 nor in the ZntB subfamily (Supporting Information Fig. S1).…”

Section: Discussionmentioning

confidence: 99%

Structure and electrostatic property of cytoplasmic domain of ZntB transporter

et al. 2009

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ZntB is the distant homolog of CorA Mg 21 transporter within the metal ion transporter superfamily. It was early reported that the ZntB from Salmonella typhimurium facilitated efflux of Zn 21 and Cd 21 , but not Mg 21 . Here, we report the 1.90 Å crystal structure of the intracellular domain of ZntB from Vibrio parahemolyticus. The domain forms a funnel-shaped homopentamer that is similar to the full-length CorA from Thermatoga maritima, but differs from two previously reported dimeric structures of truncated CorA intracellular domains. However, no Zn 21 or Cd 21 binding sites were identified in the high-resolution structure. Instead, 25 well-defined Cl 2 ions were observed and some of these binding sites are highly conserved within the ZntB family. Continuum electrostatics calculations suggest that the central pore of the funnel is highly attractive for cations, especially divalents. The presence of the bound Cl 2 ions increases the stability of cations along the pore suggesting they could be important in enhancing cation transport.

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Section: Discussionmentioning

confidence: 99%

Structure and electrostatic property of cytoplasmic domain of ZntB transporter

et al. 2009

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“…As reviewed by Schindl et al, the outer membrane pore forming the Mg 2ϩ entry side comprises the GMN motif and numerous negative electrostatic charges, which might provide ionic selectivity in those transporters possessing the GMN motif (125). As discussed by Niegowski and Eshaghi (98), some of the reported interpretative discrepancies of the three crystallographic studies may be due to deviation in members of the CorA family.…”

Section: Strategy For Identifying Novel Mg 2؉ Transportersmentioning

confidence: 99%

“…I will briefly review plant, bacterial, and yeast transporters as they relate to our newly identified mammalian Mg 2ϩ transporters, because some of these proteins share characteristics with the more ancient forms of transporters. The identification and characterization of plant, prokaryote, and yeast Mg 2ϩ transporters have been extensively reviewed elsewhere (35,70,73,138 transport (30,36,87,93,98,105,168). CorA is the primary Mg 2ϩ uptake transporter in most prokaryotes (140).…”

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confidence: 99%

Molecular identification of ancient and modern mammalian magnesium transporters

Quamme

2010

American Journal of Physiology-Cell Physiology

169

182

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handling is poorly understood.The purpose of this review is to describe some of the recent insights into the identification and function of mammalian Mg 2ϩ transporters. There is a large body of physiological evidence for mammalian Mg 2ϩ transporters, which have only begun to be identified on the molecular level (11, 51,109,117,135,148,170). In particular, I will detail, at some length, the novel Mg 2ϩ transporters that have been identified over the last several years. These will be examined from the perspective of the established and more characterized mammalian Mg 2ϩ channels, mitochondrial Mrs2 and TRPM7/6. I will briefly review plant, bacterial, and yeast transporters as they relate to our newly identified mammalian Mg 2ϩ transporters, because some of these proteins share characteristics with the more ancient forms of transporters. The identification and characterization of plant, prokaryote, and yeast Mg 2ϩ transporters have been extensively reviewed elsewhere (35, 70, 73, 138).

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“…This transporter has been suggested as being responsible for the major Mg 2+ uptake system in the Bacteria and Archaea. However, it is also believed that, in some family members, it may have a different activity than Mg 2+ transport Rainbow trout, Italy UNIUD020 Niegowski & Eshaghi, 2007). The predicted protein encoded by the sequence SSHI.59 shows homology to the product of the ZP_05668408.1 gene from Enterococcus faecium, a protein from the phosphotransferase system.…”

Section: Resultsmentioning

confidence: 99%

Application of suppressive subtractive hybridization to the identification of genetic differences between two Lactococcus garvieae strains showing distinct differences in virulence for rainbow trout and mouse

et al. 2011

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Lactococcus garvieae is the causative microbial agent of lactococcosis, an important and damaging fish disease in aquaculture. This bacterium has also been isolated from vegetables, milk, cheese, meat and sausages, from cow and buffalo as a mastitis agent, and even from humans, as an opportunistic infectious agent. In this work pathogenicity experiments were performed in rainbow trout and mouse models with strains isolated from human (L. garvieae HF) and rainbow trout (L. garvieae UNIUDO74; henceforth referred to as 074). The mean LD 50 value in rainbow trout obtained for strain 074 was 2.1¾10 2 ±84 per fish. High doses of the bacteria caused specific signs of disease as well as histological alterations in mice. In contrast, strain HF did not prove to be pathogenic either for rainbow trout or for mice. Based on these virulence differences, two suppressive subtractive hybridizations were carried out to identify unique genetic sequences present in L. garvieae HF (SSHI) and L. garvieae 074 (SSHII). Differential dot-blot screening of the subtracted libraries allowed the identification of 26 and 13 putative ORFs specific for L. garvieae HF and L. garvieae 074, respectively. Additionally, a PCR-based screening of 12 of the 26 HF-specific putative ORFs and the 13 074-specific ones was conducted to identify their presence/absence in 25 L. garvieae strains isolated from different origins and geographical areas. This study demonstrates the existence of genetic heterogeneity within L. garvieae isolates and provides a more complete picture of the genetic background of this bacterium. INTRODUCTIONLactococcus garvieae is a ubiquitous and widely distributed bacterium. It is known mainly for being the aetiological agent of lactococcosis, an important fish infection which causes great economic losses in the aquaculture industry worldwide. Many cultured fish species including rainbow trout (Oncorhynchus mykiss), olive flounder (Paralichthys olivaceus), Japanese amberjack (Seriola quinqueradiata) and black rockfish (Sebastes sp.) are affected by this Grampositive bacterium. Besides cultured fish, the bacterium has been isolated from fermented fish products (Kopermsub & Yunchalard, 2010; Paludan-Müller et al., 2002), dairy products from raw milk of different ruminants (Villani et al., 2001), vegetables (Kawanishi et al., 2007, cheeses (Alegría et al., 2009;El-Baradei et al., 2007;Fló rez & Mayo, 2006;Fortina et al., 2007), meat and sausages (Ammor et al., 2004;Barakat et al., 2000; Dàvila et al., 2006;Rantsiou et al., 2005), cows and buffaloes with mastitis (Carvalho et al., 1997;Devriese et al., 1999;Teixeira et al., 1996), cat and dog tonsils (Pot et al., 1996), and even humans, as a component of the intestinal biota (Kubota et al., 2010), and as an opportunistic pathogen (Aubin et al., 2011; Chan et al., 2011;Elliott et al., 1991;Fefer et al., 1998;Fihman et al., 2006;Furutan et al., 1991 (Michel et al., 2007). These molecular typing approaches, although providing valuable information, have some disadvantages such as the neces...

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The CorA family: Structure and function revisited

Cited by 71 publications

References 53 publications

Structure and electrostatic property of cytoplasmic domain of ZntB transporter

Structure and electrostatic property of cytoplasmic domain of ZntB transporter

Molecular identification of ancient and modern mammalian magnesium transporters

Application of suppressive subtractive hybridization to the identification of genetic differences between two Lactococcus garvieae strains showing distinct differences in virulence for rainbow trout and mouse

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