The Coprinopsis cinerea septin Cc.Cdc3 is involved in stipe cell elongation

Shioya, Tatsuhiro; Nakamura, Hitomi; Ishii, Noriyoshi; Takahashi, Naoki; Sakamoto, Yuichi; Ozaki, Noriaki; Kobayashi, Masayuki; Okano, Kiyoshi; Kamada, Takashi; Muraguchi, Hajime

doi:10.1016/j.fgb.2013.08.007

Cited by 31 publications

(23 citation statements)

References 53 publications

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“…In contrast, expression of septin genes decreases between 4_0hrPri and 5_12hrPri. This decrease might imply temporary arrest of cell growth, which is released by proper light conditions and completes in the final maturation stage [ 13 ]. Genes necessary for premeiotic DNA replication and meiotic recombination are induced prior to karyogamy (nuclear fusion), and are included in the DNA repair category observed between 5_12hrPri and 6_24hrCap as reported previously [ 24 ].…”

Section: Resultsmentioning

confidence: 99%

“…After karyogamy, meiosis proceeds in the following light period [ 6 , 9 , 10 ]. The stipe cells, in which nuclei divide without cell division [ 11 , 12 ], start to elongate around the end of meiosis [ 13 ]. As the stipe elongates, the basidiospores are produced on the outside of each basidium, arrayed on the surface of gills, making the underside of the pileus black, due to color of the mature basidiospores.…”

Section: Introductionmentioning

confidence: 99%

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Strand-Specific RNA-Seq Analyses of Fruiting Body Development in Coprinopsis cinerea

et al. 2015

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The basidiomycete fungus Coprinopsis cinerea is an important model system for multicellular development. Fruiting bodies of C. cinerea are typical mushrooms, which can be produced synchronously on defined media in the laboratory. To investigate the transcriptome in detail during fruiting body development, high-throughput sequencing (RNA-seq) was performed using cDNA libraries strand-specifically constructed from 13 points (stages/tissues) with two biological replicates. The reads were aligned to 14,245 predicted transcripts, and counted for forward and reverse transcripts. Differentially expressed genes (DEGs) between two adjacent points and between vegetative mycelium and each point were detected by Tag Count Comparison (TCC). To validate RNA-seq data, expression levels of selected genes were compared using RPKM values in RNA-seq data and qRT-PCR data, and DEGs detected in microarray data were examined in MA plots of RNA-seq data by TCC. We discuss events deduced from GO analysis of DEGs. In addition, we uncovered both transcription factor candidates and antisense transcripts that are likely to be involved in developmental regulation for fruiting.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Strand-Specific RNA-Seq Analyses of Fruiting Body Development in Coprinopsis cinerea

et al. 2015

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“…Different culture conditions and different genetic backgrounds may also play a role (Tsusu e, 1969;Iten, 1970;Kamada et al, 1978;Moore et al, 1979;McLaughlin, 1982;Rosin and Moore, 1985a,b;Hammad et al, 1993a;Lu, 2000;Liu, 2001;Liu et al, 2006). Starting from the undifferentiated secondary hyphal knots, two to four days are variably given in the literature as times required for completion of primordia formation or, quite often, timing and kinds of early events are left open (Borris, 1934;Takemaru and Kamada, 1972;Morimoto and Oda, 1973;Kamada et al, 1978;Moore et al, 1979;Ballou and Holton, 1985;Muraguchi and Kamada, 1998;Boulianne et al, 2000;Lu, 2000;Kamada, 2002;Walser et al, 2003;K€ ues et al, 2004;Liu, 2001;Kamada et al, 2010;Shioya et al, 2013).…”

Section: Coprinopsis Cinerea Psathyralleceae Agaricales Agaricomycmentioning

confidence: 99%

How do Agaricomycetes shape their fruiting bodies? 1. Morphological aspects of development

Kües

Navarro-González

2015

Fungal Biology Reviews

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“…However, the functions may not be so clear in more complex morphologies such as mushroom formation where the septin Cdc3 is needed for the extreme polarity needed for stipe cell elongation in Coprinopsis . It is possible in highly robust and rapidly polarized growth, septins become critical to driving polarity and not simply regulators of site-choice and fine-tuning of shape (104). …”

Section: How Septins Pattern and Shape Cellsmentioning

confidence: 99%

Septins and Generation of Asymmetries in Fungal Cells

Khan

McQuilken

Gladfelter

2015

Annu. Rev. Microbiol.

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Polarized growth is critical for the development and maintenance of diverse organisms and tissues, but particularly central to fungi where nutrient uptake, communication, and reproduction all rely on cell asymmetries. To achieve polarized growth, fungi spatially organize both their cytosol and cortical membranes. The septins, a family of GTP-binding proteins, have emerged as key regulators of spatial compartmentalization in fungi and other eukaryotes. By forming higher-order structures on fungal plasma membranes, septins are thought to contribute to the generation of cell asymmetries by acting as molecular scaffolds and forming diffusional barriers. Here we discuss the links between septins and polarized growth and consider molecular models for how septins contribute to cellular asymmetry in fungi.

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The Coprinopsis cinerea septin Cc.Cdc3 is involved in stipe cell elongation

Cited by 31 publications

References 53 publications

Strand-Specific RNA-Seq Analyses of Fruiting Body Development in Coprinopsis cinerea

Strand-Specific RNA-Seq Analyses of Fruiting Body Development in Coprinopsis cinerea

How do Agaricomycetes shape their fruiting bodies? 1. Morphological aspects of development

Septins and Generation of Asymmetries in Fungal Cells

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