The coordinated interaction or regulation between floral pigments and volatile organic compounds

Li, Yueqing; Bao, Tingting; Zhang, Jia; Li, Hongjie; Shan, Xiaotong; Yan, Huijun; Kimani, Shadrack; Zhang, Liangsheng; Gao, Xiang

doi:10.1016/j.hpj.2024.01.002

Cited by 2 publications

(1 citation statement)

References 332 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…is a perennial ornamental plant with a striking anemone form and exceptional floral scent cultivated for landscaping and for cut flowers; it is also regarded as a valuable germplasm resource [30]. However, despite extensive research on the structural diversity and related biosynthetic genes in a variety of plants [31][32][33][34], the bulk of volatile terpenoid and phenylpropanoid-related metabolic networks in Paeonia remains unexplored. RNA sequencing technologies have evolved into reliable and commonly used, massively parallel sequencing means for the study of molecular insights linked to various phenotypic traits without a reference genome [35,36].…”

Section: Introductionmentioning

confidence: 99%

Integration of RNA-Seq and Metabolite Analysis Reveals the Key Floral Scent Biosynthetic Genes in Herbaceous Peony

Kimani,

Wang,

Xie

et al. 2024

Horticulturae

Self Cite

View full text Add to dashboard Cite

Floral scent is an essential and genetically complex trait in herbaceous peonies (Paeonia lactiflora Pall.); however, specific genes related to metabolic and regulatory networks remain scantily studied. Our study integrated metabolite profiling and RNA-sequencing to screen floral scent biosynthetic genes. Hence, the major molecules identified by headspace collection combined with cultivar-specific GC-MS analysis were geraniol, β-caryophyllene, 2-phenylethanol (2-PE), citronellol, and 1,8-cineole. Genes related to terpenoids and 2-PE biosynthesis were identified after the assembly and annotation of the P. lactiflora transcriptomes. Eight angiosperm-specific terpene synthases (TPSs) from the TPS-a and TPS-b clades, as well as enzymes linked to 2-PE synthesis such as aromatic amino acid decarboxylase (AADC), phenylacetaldehyde reductase (PAR), and geranial reductase (GER) were identified. The biochemical analysis of the enzymes encoded by PlPAR1 and PlGER1 generated 2-PE from phenylacetaldehyde (PAld). The pairwise alignment of AADC1 reveals a splice variant lacking a 124 bp fragment, thus highlighting the possible role of alternative splicing in modulating floral scent composition. This study offers insights into the molecular-level biosynthesis of terpenoids and 2-PE in Peonia taxa, and provides the basis for the functional characterization, breeding, and bioengineering of prospective candidate genes for the production of floral volatiles in the Paeonia genus.

show abstract