The cooperation of <i>cis</i>-elements during <i>M-cadherin</i> promoter activation

Lin, Yung Jui; Kao, Chien-Han; Hsiao, Sheng Pin; Chen, Shen Liang

doi:10.1042/bcj20200535

Cited by 5 publications

(2 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The CRISPRi system was established according to previous studies [41], and the detailed protocol has been published in our previous study [42]. Briefly, C2C12 myoblasts were over-expressed with KRAB-dCas9-P2A-mCherry fusion gene via lentivirus infection and infected C2C12 myoblasts were later serially diluted in a 96-well titer plate to identify red clones expressing KRAB-dCas9-P2A-mCherry.…”

Section: Knockdown Of Pex3 By Crispr Interference (Crispri)mentioning

confidence: 99%

Reciprocal Regulation of Peroxisome Biogenesis and Myogenic Factors Is Critical for Myogenesis

Wu,

Chen,

Hsiao

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

Mitochondria (MITO) and peroxisomes (PEXO) are the major organelles involved in the oxidative metabolism of cells, but detailed examination of their dynamics and functional adaptations during skeletal muscle (SKM) development (myogenesis) is still lacking. In this study, we found that during myogenesis, MITO DNA, ROS level, and redox ratio increased in myotubes, but the membrane potential (Δψm) and ATP content reduced, implying that the MITO efficiency might reduce during myogenesis. The PEXO number and density both increased during myogenesis, which probably resulted from the accumulation and increased biogenesis of PEXO. The expression of PEXO biogenesis factors was induced during myogenesis in vitro and in utero, and their promoters were also activated by MyoD. Knockdown of the biogenesis factors Pex3 repressed not only the PEXO density and functions but also the levels of MITO genes and functions, suggesting a close coupling between PEXO biogenesis and MITO functions. Surprisingly, Pex3 knockdown by the CRISPRi system repressed myogenic differentiation, indicating critical involvement of PEXO biogenesis in myogenesis. Taken together, these observations suggest that the dynamics and functions of both MITO and PEXO are coupled with each other and with the metabolic changes that occur during myogenesis, and these metabolic couplings are critical to myogenesis.

show abstract

Section: Knockdown Of Pex3 By Crispr Interference (Crispri)mentioning

confidence: 99%

Reciprocal Regulation of Peroxisome Biogenesis and Myogenic Factors Is Critical for Myogenesis

Wu,

Chen,

Hsiao

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…Finally, PBX1 is also a cofactor for proteins implicated in myoblast differentiation, such as M‐cadherin, (Y.‐J. Lin et al, 2021).…”

Section: Shedding Light On Human Pbx1‐induced Phenotypes Using Animal...mentioning

confidence: 99%

The TALE never ends: A comprehensive overview of the role of PBX1, a TALE transcription factor, in human developmental defects

et al. 2022

View full text Add to dashboard Cite

PBX1 is a highly conserved atypical homeodomain transcription factor (TF) belonging to the TALE (three amino acid loop extension) family. Dimerized with other TALE proteins, it can interact with numerous partners and reach dozens of regulating sequences, suggesting its role as a pioneer factor. PBX1 is expressed throughout the embryonic stages (as early as the blastula stage) in vertebrates. In human, PBX1 germline variations are linked to syndromic renal anomalies (CAKUTHED). In this review, we summarized available data on PBX1 functions, PBX1‐deficient animal models, and PBX1 germline variations in humans. Two types of genetic alterations were identified in PBX1 gene. PBX1 missense variations generate a severe phenotype including lung hypoplasia, cardiac malformations, and sexual development defects (DSDs). Conversely, truncating variants generate milder phenotypes (mainly cryptorchidism and deafness). We suggest that defects in PBX1 interactions with various partners, including proteins from the HOX (HOXA7, HOXA10, etc.), WNT (WNT9B, WNT3), and Polycomb (BMI1, EED) families are responsible for abnormal proliferation and differentiation of the embryonic mesenchyme. These alterations could explain most of the defects observed in humans. However, some phenotype variability (especially DSDs) remains poorly understood. Further studies are needed to explore the TALE family in greater depth.

show abstract

Characterization of three novel cell lines derived from the brain of spotted sea bass: Focusing on cell markers and susceptibility toward iridoviruses

Liu¹,

Ma²,

Liang³

2022

Fish & Shellfish Immunology

View full text Add to dashboard Cite

The cooperation of cis-elements during M-cadherin promoter activation

Cited by 5 publications

References 48 publications

Reciprocal Regulation of Peroxisome Biogenesis and Myogenic Factors Is Critical for Myogenesis

Reciprocal Regulation of Peroxisome Biogenesis and Myogenic Factors Is Critical for Myogenesis

The TALE never ends: A comprehensive overview of the role of PBX1, a TALE transcription factor, in human developmental defects

Characterization of three novel cell lines derived from the brain of spotted sea bass: Focusing on cell markers and susceptibility toward iridoviruses

Contact Info

Product

Resources

About