The control of Staphylococcus epidermidis biofilm formation and in vivo infection rates by covalently bound furanones

Hume, Emma B.H.; Baveja, J.K; Muir, Benjamin W.; Schubert, T.; Kumar, Naresh; Kjelleberg, Staffan; Griesser, Hans J.; Thissen, Helmut; Read, RW; Poole-Warren, Laura A.; Schindhelm, Klaus; Willcox, Mark

doi:10.1016/j.biomaterials.2004.01.048

Cited by 146 publications

(90 citation statements)

References 31 publications

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“…Consequently, the reduction of pioneer bacterial attachment affects the successive stages of biofilm formation, which is essential in preventing device-associated infection. This has been suggested in several studies addressing antimicrobial agents loaded biomaterials, e.g., furanone-incorporated polystyrene discs, nanoHA-ZnO composite, Ti/PBA-CHX, ZnO/PVC composite and polyurethane nanocomposites-CHX [3,20,28,46,47].…”

Section: Discussionmentioning

confidence: 90%

Anti-sessile bacterial and cytocompatibility properties of CHX-loaded nanohydroxyapatite

Barros

Grenho

Fernandes

et al. 2015

Colloids and Surfaces B: Biointerfaces

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Nanohydroxyapatite possesses exceptional biocompatibility and bioactivity regarding bone cells and tis-sues, justifying its use as a coating material or as a bone substitute. Unfortunately, this feature may also encourage bacterial adhesion and biofilm formation. Surface functionalization with antimicrobials is a promising strategy to reduce the likelihood of bacterial infestation and colonization on medical devices. Chlorhexidine digluconate is a common and effective antimicrobial agent used for a wide range of medical applications. The purpose of this work was the development of a nanoHA biomaterial loaded with CHX to prevent surface bacterial accumulation and, simultaneously, with good cytocompatibility, for application in the medical field. CHX (5-1500 mg/L) was loaded onto nanoHA discs and the materials were evaluated for CHX adsorption and release profile, physic-chemical features, antibacterial activity against Escherichia coli, Staphylococcus aureus and Staphylococcus epidermidis, and cytocompatibility toward L929 fibroblasts. Results showed that the adsorption of CHX on nanoHA surface occurred by electrostatic inter-actions between the cationic group of CHX and the phosphate group of nanoHA. The release of CHX from CHX-loaded nanoHA showed a fast initial rate followed by a slower kinetics release, due to constraints caused by dilution and diffusion-limiting processes. NanoHA.50 to nanoHA.1500 showed strong anti-sessile activity, inhibiting bacterial adhesion and the biofilm formation. CHX-nanoHA caused a dose-and time-dependent inhibitory effect on the proliferation of fibroblasts for nanoHA.100 to nanoHA.1500. Cellular behavior on 2 nanoHA.5 and nanoHA.50 was similar to control. Therefore, CHX-loaded nanoHA surfaces appear as a promising alternative to prevention of devices-related infections.

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Section: Discussionmentioning

confidence: 90%

Anti-sessile bacterial and cytocompatibility properties of CHX-loaded nanohydroxyapatite

Barros

Grenho

Fernandes

et al. 2015

Colloids and Surfaces B: Biointerfaces

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“…It remains unclear, though, how E. coli's chemotaxis and flagellar responses to AI-2 are propagated, since the Lsr system, the only known AI-2 sensory pathway in enteric bacteria, has not been directly linked to these chemosensory systems. AI-2 signaling in Gram-positive bacteria is even less clear, since no signaling pathways have been described, yet staphylococcal biofilms were inhibited from growing on materials used in medical devices when furanone compounds were integrated into them (252). Likewise, synthetic furanones and furanone analogues incorporating thiophenone moieties decreased staphylococcal biofilm formation mediated by AI-2 (245,253).…”

Section: Natural-product Qs Inhibitorsmentioning

confidence: 99%

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

LaSarre

Federle

2013

Microbiol Mol Biol Rev

677

556

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SUMMARY Cell-cell communication, or quorum sensing, is a widespread phenomenon in bacteria that is used to coordinate gene expression among local populations. Its use by bacterial pathogens to regulate genes that promote invasion, defense, and spread has been particularly well documented. With the ongoing emergence of antibiotic-resistant pathogens, there is a current need for development of alternative therapeutic strategies. An antivirulence approach by which quorum sensing is impeded has caught on as a viable means to manipulate bacterial processes, especially pathogenic traits that are harmful to human and animal health and agricultural productivity. The identification and development of chemical compounds and enzymes that facilitate quorum-sensing inhibition (QSI) by targeting signaling molecules, signal biogenesis, or signal detection are reviewed here. Overall, the evidence suggests that QSI therapy may be efficacious against some, but not necessarily all, bacterial pathogens, and several failures and ongoing concerns that may steer future studies in productive directions are discussed. Nevertheless, various QSI successes have rightfully perpetuated excitement surrounding new potential therapies, and this review highlights promising QSI leads in disrupting pathogenesis in both plants and animals.

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“…Sabe-se que algumas espécies de estafilococos produzem um muco ou biofilme que permite à bactéria aderir às superfícies, sendo importante para a sua colonização. A produção de biofilme é considerada como um fator de virulência (Veenstra et al 1996, Vuong & Otto 2002, tornandoas menos acessíveis ao sistema de defesa do organismo e aos antimicrobianos (Arciola et al 2002, Hume et al 2004. Esse mecanismo inibe a quimiotaxia, fagocitose, proliferação de linfócitos e limita a atuação dos macrófagos (Vasudevan et al 2003).…”

Section: Discussionunclassified

Perfil de resistência a antimicrobianos de Staphylococcus spp. isolados de frangos de corte e poedeiras comerciais no estado de Pernambuco

et al. 2011

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Este estudo foi realizado com o objetivo de pesquisar Staphylococcus spp. de frangos de corte sadios e frangos de corte e poedeiras comerciais que apresentassem sinais clínicos respiratórios. Foram colhidos swabs dos seios infra-orbitários de 55 frangos de corte sadios, 35 com sinais respiratórios, e 30 poedeiras comerciais também com sinais respiratórios. Cada amostra foi composta por um "pool" de cinco aves, totalizando 24 amostras coletadas de 24 granjas comerciais. Para o isolamento foi utilizado o exame bacteriológico, com posterior avaliação das características morfológicas, tintoriais e bioquímica para determinação da espécie. Verificou-se a produção de hemólise, formação de biofilme em ágar Vermelho Congo (ACR), detecção do gene mecA pela PCR e avaliação da suscetibilidade a 13 drogas antimicrobianas. Das 24 amostras processadas, foram isolados 16 Staphylococcus, cinco isolados foram coagulase-positiva (SCP) e 11 coagulase-negativa (SCN), e nos testes de hemólise e formação de biofilme, três isolados apresentaram-se hemolíticos e seis foram positivos, respectivamente. Na avaliação por meio da PCR, para detecção do gene mecA todos os isolados apresentaram resultados negativo. Observaram-se que 15 isolados foram resistentes a cinco ou mais antibióticos, e que as drogas associadas apresentaram melhor perfil de sensibilidade. A resistência a antimicrobianos e cepas produtoras de biofilme podem interferir na resposta terapêutica de aves que apresentam sinais clínicos.

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The control of Staphylococcus epidermidis biofilm formation and in vivo infection rates by covalently bound furanones

Cited by 146 publications

References 31 publications

Anti-sessile bacterial and cytocompatibility properties of CHX-loaded nanohydroxyapatite

Anti-sessile bacterial and cytocompatibility properties of CHX-loaded nanohydroxyapatite

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

Perfil de resistência a antimicrobianos de Staphylococcus spp. isolados de frangos de corte e poedeiras comerciais no estado de Pernambuco

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