The control of root growth by reactive oxygen species in Salix nigra Marsh. seedlings

Causin, Humberto F.; Roqueiro, Gonzalo; Petrillo, Ezequiel; Láinez, Verónica; Pena, Liliana B.; Marchetti, Cintia F.; Gallego, Susana M.; Maldonado, Sara

doi:10.1016/j.plantsci.2011.08.012

Cited by 31 publications

(16 citation statements)

References 44 publications

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“…Germination percentage improved almost to the level of control when DEDTC was combined with H 2 O 2 (10 mM). However, treatment with KI (50 mM) and DMTU (50 mM), both H 2 O 2 specific scavengers, 23 could not inhibit germination (Fig. 4C).…”

Section: Resultsmentioning

confidence: 92%

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Superoxide and its metabolism during germination and axis growth ofVigna radiata(L.) Wilczek seeds

Singh

Chaudhuri²,

Kar

2014

Plant Signaling & Behavior

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Involvement of reactive oxygen species in regulation of plant growth and development is recently being demonstrated with various results depending on the experimental system and plant species. Role of superoxide and its metabolism in germination and axis growth was investigated in case of Vigna radiata seeds, a non-endospermous leguminous species having epigeal germination, by studying the effect of different reactive oxygen species (ROS) inhibitors, distribution of O2(•)- and H2O2 and ROS enzyme profile in axes. Germination percentage and axis growth were determined under treatment with ROS inhibitors and scavengers. Localization of O2(•)- and H2O2 was done using nitroblue tetrazolium (NBT) and 3,3',5,5'-tetramethyl benzidine dihydrochloride hydrate (TMB), respectively. Apoplastic level of O2(•)- was monitored by spectrophotometric analysis of bathing medium of axes. Profiles of NADPH oxidase and superoxide dismutase (SOD) were studied by in-gel assay. Germination was retarded by treatments affecting ROS level except H2O2 scavengers, while axis growth was retarded by all. Superoxide synthesis inhibitor and scavenger prevented H2O2 accumulation in axes in later phase as revealed from TMB staining. Activity of Cu/Zn SOD1 was initially high and declined thereafter. Superoxide being produced in apoplast possibly by NADPH oxidase activity is further metabolized to (•)OH via H2O2. Germination process depends possibly on (•)OH production in the axes. Post-germinative axis growth requires O2(•)- while the differentiating zone of axis (radicle) requires H2O2 for cell wall stiffening.

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Section: Resultsmentioning

confidence: 92%

“…4C) Retardation of Phase II axis growth ( Fig. 4D and Table 3) DMTU hydrogen peroxide scavenger 23 10 No significant inhibition or promotion in any observations 50…”

mentioning

confidence: 95%

Superoxide and its metabolism during germination and axis growth ofVigna radiata(L.) Wilczek seeds

Singh

Chaudhuri²,

Kar

2014

Plant Signaling & Behavior

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“…This result indicates that root development is very important for the drought stress tolerance and also we are interested in the mechanism how rice roots overcome damage caused by prolonged WD stress. It was known that processing of reactive oxygen species (ROS) play important roles in root development of other plant species (Causin et al, 2012; Zhao et al, 2016). …”

Section: Resultsmentioning

confidence: 99%

OsPhyB-Mediating Novel Regulatory Pathway for Drought Tolerance in Rice Root Identified by a Global RNA-Seq Transcriptome Analysis of Rice Genes in Response to Water Deficiencies

et al. 2017

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Water deficiencies are one of the most serious challenges to crop productivity. To improve our understanding of soil moisture stress, we performed RNA-Seq analysis using roots from 4-week-old rice seedlings grown in soil that had been subjected to drought conditions for 2–3 d. In all, 1,098 genes were up-regulated in response to soil moisture stress for 3 d, which causes severe damage in root development after recovery, unlikely that of 2 d. Comparison with previous transcriptome data produced in drought condition indicated that more than 68% of our candidate genes were not previously identified, emphasizing the novelty of our transcriptome analysis for drought response in soil condition. We then validated the expression patterns of two candidate genes using a promoter-GUS reporter system in planta and monitored the stress response with novel molecular markers. An integrating omics tool, MapMan analysis, indicated that RING box E3 ligases in the ubiquitin-proteasome pathways are significantly stimulated by induced drought. We also analyzed the functions of 66 candidate genes that have been functionally investigated previously, suggesting the primary roles of our candidate genes in resistance or tolerance relating traits including drought tolerance (29 genes) through literature searches besides diverse regulatory roles of our candidate genes for morphological traits (15 genes) or physiological traits (22 genes). Of these, we used a T-DNA insertional mutant of rice phytochrome B (OsPhyB) that negatively regulates a plant's degree of tolerance to water deficiencies through the control of total leaf area and stomatal density based on previous finding. Unlike previous result, we found that OsPhyB represses the activity of ascorbate peroxidase and catalase mediating reactive oxygen species (ROS) processing machinery required for drought tolerance of roots in soil condition, suggesting the potential significance of remaining uncharacterized candidate genes for manipulating drought tolerance in rice.

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“…Roots were stained with 1 μg/mL DAPI for 30 min and analyzed with an Olympus BX60 fluorescence microscope (Ex/Em: 365/ 420-540 nm; Zeiss, Jena, Germany) (Tsukagoshi 2012). To visualize the localization and/or rate of O 2 · − production, seedlings were stained for 15 min with a solution of 0.1 % NBT in 50 mM sodium phosphate buffer [pH 7.5] (Causin et al 2012).…”

Section: Treatment Of a Brasilense With Papainmentioning

confidence: 99%

Oxidative and antioxidative responses in the wheat-Azospirillum brasilense interaction

et al. 2015

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Azospirillum is a plant growth-promoting rhizobacteria (PGPR) able to enhance the growth of wheat. The aim of this study was to test the effect of Azospirillum brasilense cell wall components on superoxide (O2·(-)) production in wheat roots and the effect of oxidative stress on A. brasilense viability. We found that inoculation with A. brasilense reduced O2·(-) levels by approx. 30 % in wheat roots. Inoculation of wheat with papain-treated A. brasilense, a Cys protease, notably increased O2·(-) production in all root tissues, as was observed by the nitro blue tetrazolium (NBT) reduction. However, a 24-h treatment with rhizobacteria lipopolysaccharides (50 and 100 μg/mL) alone did not affect the pattern of O2·(-) production. Analysis of the effect of plant cell wall components on A. brasilense oxidative enzyme activity showed no changes in catalase activity but a decrease in superoxide dismutase activity in response to polygalacturonic acid treatment. Furthermore, A. brasilense growth was only affected by high concentrations of H2O2 or paraquat, but not by sodium nitroprusside. Our results suggest that rhizobacterial cell wall components play an important role in controlling plant cell responses and developing tolerance of A. brasilense to oxidative stress produced by the plant.

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The control of root growth by reactive oxygen species in Salix nigra Marsh. seedlings

Cited by 31 publications

References 44 publications

Superoxide and its metabolism during germination and axis growth ofVigna radiata(L.) Wilczek seeds

Superoxide and its metabolism during germination and axis growth ofVigna radiata(L.) Wilczek seeds

OsPhyB-Mediating Novel Regulatory Pathway for Drought Tolerance in Rice Root Identified by a Global RNA-Seq Transcriptome Analysis of Rice Genes in Response to Water Deficiencies

Oxidative and antioxidative responses in the wheat-Azospirillum brasilense interaction

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