The control of nuclear and cytoplasmic synthesis by the nucleo-cytoplasmic ratio in Saccharomyces

The Integrated Cell

“…Bud 2a ceased to grow after 35 minutes indicating depletion of nutritional materials. During the period of constant volume before budding, fluctuations between 1.05 and 1.08 ( Haddad and Lindegren 1953, Lindegren and Haddad 1953) in density occurred indicating that little, if any increase in total weight could have occurred.…”

“…Not only can gene-cotrolled abilities for the splitting of a disaccharide be lost by dissimilation but yeasts (Lindegren and Skoog 1947) incapable of fermenting glucose can be produced by growth on lactate and synthetic medium although these variants revert to glucose fermentation rather readily. These experiments indicate that the assumption (4) that dissimilation does not affect the expression of the gene was not correct.…”

“…3) Some genes have several manifestations, some of which are extremely stable, while others convert frequently. The MZ allele in Saccharomyces ( Lindegren and Lindegren 1953) has at least five manifestations involving ability to adapt to maltose, turanose, sucrose, methyl-a-D-glucopyranoside and melezitose. The ability to adapt to maltose is lost rarely, while the ability to adapt to melezitose is lost readily.…”

“…In respect to assigning the proper role of the gene and the cell in evo lution, the gene MZ (Lindegren et al, 1957) in yeast which controls the adaptive synthesis of the enzyme melezitase is of special interest. The synthesis of melezitase occurs when cells carrying the totipotent MZ gene are exposed to any one of 5 inducers: turanose (T), maltose (M), sucrose (S), methyl a-D-glucopyranoside (G) and melezitose (Z).…”