The contribution of tellurite resistance genes to the fitness of Escherichia coli uropathogenic strains

Valková, Danka; Valkovičová, Lenka; Vávrová, Silvia; Kovacóvá, E; Mravec, Jozef; Turňa, Ján

doi:10.2478/s11535-007-0019-9

Cited by 24 publications

(34 citation statements)

References 22 publications

(24 reference statements)

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“…Interestingly, TerD and TerE proteins were among the most abundant proteins of EHEC O157:H7 proteome (Table 1). A study performed with Uropathogenic E. coli (UPEC) isolates showed that the introduction of the ter gene cluster contributes to improve bacterial fitness inside macrophages (44). On the other hand, Yin et al (45) demonstrated that ter genes contribute to adherence of EHEC O157:H7 to epithelial cells.…”

Section: Resultsmentioning

confidence: 99%

Quantification of EnterohemorrhagicEscherichia coliO157:H7 proteome using TMT-Based Analysis

Bei

Amigo

et al. 2018

Preprint

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25Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a human pathogen responsible for 26 diarrhea, hemorrhagic colitis and hemolytic uremic syndrome (HUS). EHEC infection is 27 distributed worldwide and numerous outbreaks of diseases caused by enterohemorrhagic 28 have been reported. To promote a comprehensive insight into the molecular basis of EHEC 29 O157:H7 physiology and pathogenesis, the combined proteome of EHEC O157:H7 strains, 30 Clade 8 and Clade 6 isolated from cattle in Argentina, and the standard EDL933 (clade 3) 31 strain has been analyzed. TMT (Tandem Mass Tags)-based quantitative proteomic and 32 emPAI analyses were performed to estimate the protein abundance in EHEC proteome. 33 2,234 non-redundant proteins of EHEC O157:H7 were identified. A comparison of this 34 result with in silico data of EHEC O157:H7 genome showed that approximately 40% of the 35 predicted proteome of this pathogen were covered. According to the emPAI analysis, 85 36 proteins were among the most abundant (e.g. GAPDH, FliC H-antigen, Enolase, and 37 GroEL). Tellurite resistance proteins were also highly abundant. COG analysis showed that 38 although most of the identified proteins are related to cellular metabolism, the majority of 39 the most abundant proteins are associated with translation processes. A KEGG enrichment 40 analysis revealed that Glycolysis / Gluconeogenesis was the most significant pathway. On 41 the other hand, the less abundant detected proteins are those related to DNA processes, cell 42 respiration and prophage. Among the proteins that composed the Type III Secretion 43 System, the most abundant protein was EspA. Altogether, the results show a subset of 44 important proteins that contribute to physiology and pathogenicity of EHEC O157:H7. 45 IMPORTANCE 46 3 The study of the abundance of proteins present within a complex mixture of proteins in a 47 cell, under different conditions, can provide important information about the activities of 48 individual protein components and protein networks that are cornerstones for the 49 comprehension of physiological adaptations in response to biological demands promoted 50 by environmental changes. We generated a comprehensive and accurate quantitative list of 51 EHEC O157:H7 proteome, which provides a description of the most abundant proteins 52 produced by this pathogen that were related to physiology and pathogenesis of EHEC. This 53 study provides information and extends the understanding on functional genomics and the 54 biology of this pathogen.55 Keywords: EHEC, Tandem mass tags (TMT), protein quantitation, bacterial proteomic 56 57 58 Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a zoonotic pathogen belonging to 59 Shiga toxin-producing E. coli (STEC) and responsible for different diseases as diarrhea, 60 hemorrhagic colitis and hemolytic uremic syndrome (HUS). HUS is distributed worldwide 61 and considered to be a public health problem in several countries (1,2). Unfortunately, 62 Argentina is the country with the highest incidence of HUS i...

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Section: Resultsmentioning

confidence: 99%

Quantification of EnterohemorrhagicEscherichia coliO157:H7 proteome using TMT-Based Analysis

Bei

Amigo

et al. 2018

Preprint

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“…Although the plasmid-borne or chromosomally located ter clusters have been extensively studied, the real functions of the Te R genes are not well understood. The introduction of the ter operon from uropathogenic E. coli KL53 into non-pathogenic and uropathogenic E. coli (ter-gene free) showed that the presence of the ter loci improved the fitness of bacterial strains by enabling them to survive inside macrophages (Valkova et al 2007). Deletion of the Te R region reduced the ability of the strain to adhere to epithelial cells in vitro and in ligated pig ileal loops (Yin et al 2009).…”

Section: Discussionmentioning

confidence: 99%

“…The whole determinant was cloned using in vivo methods based on the miniMu derivative cloning system, resulting in the pNT3B plasmid (Kormutakova et al 2000;Tu et al 2001;Vavrova et al 2006). The presence of the ter operon improved the fitness of bacterial strains by enabling them to escape from macrophage attack (Valkova et al 2007). In the present study, we present the biological function of the TerW protein.…”

Section: Introductionmentioning

confidence: 99%

The role of TerW protein in the tellurite resistance of uropathogenic Escherichia coli

et al. 2011

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Abstract:The ter operon, which is found on a large conjugative plasmid, pTE53, from the uropathogenic strain Escherichia coli KL53, mediates heavy metal ion resistance to tellurium compounds. Here we present the function of the terW gene, which is transcribed from its own promoter in the opposite direction to the terZABCDE loci and encodes a protein of 155 amino acids. A TerW protein containing an N-terminal His-tag was overexpressed, purified and analysed for the in vitro DNA-binding. Green fluorescent protein fusions, a fluorimetric assay, nonradioactive gel retardation and capillary electrophoresis footprinting were used to examine the interaction between the bioinformatically-predicted DNA-binding region and TerW. Here we show that TerW binds specifically to the potential promoter region of the terZABCDE genes, which are responsible for the tellurite resistance of the Escherichia coli host cells. Our results suggest that the transcriptional regulation of ter genes is operon-like.

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“…Although the exact function of this locus in vivo is not clear, orthologous genes are required for tellurite resistance in some bacteria (71)(72)(73). Tellurite is also thought to induce oxidative stress and tellurite resistance might be the result of decreased sensitivity to the oxidative stress (74).…”

Section: Tellurite Resistance Genesmentioning

confidence: 99%

The PhoPQ two-component system is the major regulator of cell surface properties, stress responses and plant-derived substrate utilisation during development ofPectobacterium versatile-host plant pathosystem

Kravchenko

Gogoleva

Kolubako

et al. 2020

Preprint

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The PhoPQ two-component system, originally described in pectobacteria as PehRS, was previously shown to regulate a single gene, pehA. Using an insertional phoP mutant of Pectobacterium versatile, we demonstrate that PhoP controls a regulon of at least 116 genes with a large fraction of regulon members specific for pectobacteria. The functions performed by the PhoP controlled genes include transport and metabolism of plant-derived carbon sources (polygalacturonate, arabinose and citrate), modification of bacterial cell envelope and stress resistance. High concentrations of calcium and magnesium ions were found to abolish the PhoPQdependent transcription activation. Reduced PhoP expression and minimisation of PhoP dependence of regulon members' expression in the cells isolated from rotten potato tuber tissues suggest that PhoPQ system may adjust expression levels of multiple virulence-related genes during the course of P. versatile-host plant pathosystem development.PehR was originally described as a transcriptional activator of the pehA gene encoding endopolygalacturonase, a major virulence factor in soft rot bacteria (16). PehR is a response regulator that forms a two-component sensory system (TCS) with the membrane histidine kinase PehS. In the SCC3193 strain, which is currently classified as Pectobacterium parmentieri, inactivation of either pehR or pehS resulted in reduced virulence and Ca 2+ was reported as the ligand detected by the PehS sensor (16,17). It was also suggested that the PehRS system is responsible for the decrease of the polygalacturonase and increase of the pectate lyase activities in response to Ca 2+ released from the degraded cell walls (17).The pectobacterial PehRS two-component system received very little attention since its discovery two decades ago. However, much information has been accumulated about the orthologous system called PhoPQ in other bacteria from the order Enterobacetrales. In Dickeya dadantii, which belongs to the Pectobacteriaceae family together with Pve, PhoPQ TCS was shown to control pellicle formation, motility, resistance to cationic antimicrobial peptides and expression of pectate lyases in response to Mg 2+ concentration (18,19). The PhoPQ system has been thoroughly characterised in Salmonella and to a lesser extent in few more Enterobacteriaceae species including Escherichia coli and Yersinia spp. (see (20) for a review). The PhoQ sensor histidine kinase activates PhoP by phosphorylation in response to several stimuli, including low Mg 2+ concentration (21), cationic peptides (22), acidity (23) and high osmolarity (24). PhoP binds to direct repeats with a consensus gGTTTA which seems to be well conserved in enterobacteria (25,26). The regulon composition nevertheless varies widely even between closely related bacteria. In Salmonella enterica, over a hundred genes are regulated by PhoP, but only three of them (phoP, phoQ and slyB) are always under PhoP control in other enterobacteria. Some regulon members are shared by several species, but most PhoP controlled gene...

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The contribution of tellurite resistance genes to the fitness of Escherichia coli uropathogenic strains

Cited by 24 publications

References 22 publications

Quantification of EnterohemorrhagicEscherichia coliO157:H7 proteome using TMT-Based Analysis

Quantification of EnterohemorrhagicEscherichia coliO157:H7 proteome using TMT-Based Analysis

The role of TerW protein in the tellurite resistance of uropathogenic Escherichia coli

The PhoPQ two-component system is the major regulator of cell surface properties, stress responses and plant-derived substrate utilisation during development ofPectobacterium versatile-host plant pathosystem

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