The contribution of platelets in the production of cryoprecipitates for use in a fibrin glue

Rock, G.; Neurath, Doris; Lu, Mang; Alharbi, A.; Freedman, Mark S.

doi:10.1111/j.1423-0410.2006.00788.x

Cited by 15 publications

(7 citation statements)

References 14 publications

(18 reference statements)

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“…With special focus on a fibrin-based cell-delivery matrix which covers clinical requirements such as autologous tissue material and easy handling within an operation room, we used an autologous plasma clot [7]. This plasma clot still contains platelets and supports embedded cells with platelet-derived cell growth factors [8]. However, we also observed non-viable cells after the clotting process within this matrix.…”

Section: Discussionmentioning

confidence: 99%

Fixation of porous calcium phosphate with expanded bone marrow cells using an autologous plasma clot

Schildhauer

Seybold

Geßmann

et al. 2007

Materialwissenschaft Werkst

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Multipotent human mesenchymal stromal cells (hMSCs) are currently the most promising cell type for regenerative medicine and tissue engineering. For clinical applications with special focus on fracture repair a method to deliver in vitro-expanded bone marrow cells to the fracture site is presented. Autologous blood plasma clotting was used to fix expanded cells and synthetic tricalcium phosphate particles. A jelly-like but solid composite matrix was obtained which could easily be handled by the surgeon. The majority of embedded cells remained viable after the plasma gelation process, as was determined by fluorescence microscopy. Experimental cell culture over a period of 15 days showed proliferating mesenchymal stromal cells within the plasma clot.Multipotente mesenchymale Stromazellen gelten als viel versprechender Zelltyp für regenerative Medizin und Tissue Engineering. Im Rahmen klinischer Anwendungen mit Fokus auf die Frakturheilung wird eine Methode zur Versorgung mit in vitro-expandierten Knochenmarkszellen in Frakturnähe vorgestellt. Zur Fixierung der expandierten Zellen zusammen mit synthetischer Tricalciumphosphat-Granula wurde autologes Blutplasma verwendet und so eine gellee-artige aber feste Komposit-Matrix hergestellt, die vom Chirurgen einfach zu handhaben war. Wie mittels Fluoreszenzmikroskopie bestimmt wurde, blieb die Mehrzahl der eingebetteten Zellen nach dem Gelierungsprozess vital. Eine anschließende experimentelle Zellkultur für weitere 15 Tage zeigte im Plasmakoagulat proliferierende mesenchymale Stromazellen.

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Section: Discussionmentioning

confidence: 99%

Fixation of porous calcium phosphate with expanded bone marrow cells using an autologous plasma clot

Schildhauer

Seybold

Geßmann

et al. 2007

Materialwissenschaft Werkst

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“…In addition, the biological half-life of bioactive molecules may be prolonged after binding to the fibrin matrix [21]. The platelet factors are well known to stimulate the proliferation of MSC [16]. The osteopromoting factors which were derived from activated leukocytes are still not identified.…”

Section: Discussionmentioning

confidence: 98%

“…It was reported that generated thrombin is able to activate human leukocytes via the AP-1-pathway [15]. In addition, there are a multitude of bioactive factors released from platelets which were rapidly activated during the clotting process [16]. This plasma clot still contains platelets especially due to the presence within the PBMC fraction and supports embedded cells with platelet-derived cell growth factors such as bFGF, VEGF, PDGF, or TGF-ß [17].…”

Section: Discussionmentioning

confidence: 99%

Osteogenic differentiation of human mesenchymal stromal cells is promoted by a leukocytes containing fibrin matrix

Seybold

Schildhauer

Geßmann

et al. 2010

Langenbecks Arch Surg

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“…Fibrinogen derived by cryoprecipitation has platelet-derived growth factors such as TGF-b1, -b2, and -b3, PDGF, and VEGF, 36 and the combination of recombinant TGF-b1 exposure from CM and TGF-b1 and -b3 exposure from the fibrin, may have influenced the progression toward a stable chondrocyte phenotype.…”

Section: Watts Et Almentioning

confidence: 99%

A Comparison of Three-Dimensional Culture Systems to EvaluateIn VitroChondrogenesis of Equine Bone Marrow-Derived Mesenchymal Stem Cells

Watts

Ackerman-Yost

Nixon

2013

Tissue Engineering Part A

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Objective: To compare in vitro three-dimensional (3D) culture systems that model chondrogenesis of bone marrow-derived mesenchymal stem cells (MSCs). Methods: MSCs from five horses 2-3 years of age were consolidated in fibrin 0.3% alginate, 1.2% alginate, 2.5 · 10 5 cell pellets, 5 · 10 5 cell pellets, and 2% agarose, and maintained in chondrogenic medium with supplemental TGF-b1 for 4 weeks. Pellets and media were tested at days 1, 14, and 28 for gene expression of markers of chondrogenic maturation and hypertrophy (ACAN, COL2B, COL10, SOX9, 18S), and evaluated by histology (hematoxylin and eosin, Toluidine Blue) and immunohistochemistry (collagen type II and X). Results: alginate, fibrin alginate (FA), and both pellet culture systems resulted in chondrogenic transformation. Adequate RNA was not obtained from agarose cultures at any time point. There was increased COL2B, ACAN, and SOX9 expression on day 14 from both pellet culture systems. On day 28, increased expression of COL2B was maintained in 5 · 10 5 cell pellets and there was no difference in ACAN and SOX9 between FA and both pellet cultures. COL10 expression was significantly lower in FA cultures on day 28. Collagen type II was abundantly formed in all culture systems except alginate and collagen type X was least in FA hydrogels. Conclusion: equine MSCs respond to 3D culture in FA blended hydrogel and both pellet culture systems with chondrogenic induction. For prevention of terminal differentiation and hypertrophy, FA culture may be superior to pellet culture systems.

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The contribution of platelets in the production of cryoprecipitates for use in a fibrin glue

Abstract: Platelets do not significantly increase the concentration of the usual constituents of cryoprecipitate; however, the levels of platelet-derived growth factor are markedly enhanced. Therefore, there are advantages for using PRP to enhance the growth of new tissue.

Cited by 15 publications

References 14 publications

Fixation of porous calcium phosphate with expanded bone marrow cells using an autologous plasma clot

Fixation of porous calcium phosphate with expanded bone marrow cells using an autologous plasma clot

Osteogenic differentiation of human mesenchymal stromal cells is promoted by a leukocytes containing fibrin matrix

A Comparison of Three-Dimensional Culture Systems to EvaluateIn VitroChondrogenesis of Equine Bone Marrow-Derived Mesenchymal Stem Cells

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