The contribution of pH‐dependent mechanisms to fatigue at different intensities in mammalian single muscle fibres

Abstract

The contribution of intracellular pH (pHi) to the failure of Ca2+ release and inhibition of contractile proteins observed during fatigue was assessed in single intact mouse muscle fibres at 22 °C. Fatigue was induced by repeated tetani at intensities designed to induce different levels of intracellular acidosis. Force and either intracellular free Ca2+ concentration ([Ca2+]i; measured using indo‐1) or pHi (measured using SNARF‐1) were recorded in fibres fatigued at two different intensities. Intensity was varied by the repetition rate of tetani and quantified by the duty cycle (the fraction of time when the muscle was tetanized). Stimulation at the low intensity (duty cycle ∼0.1) reduced force to 30 % of initial values in 206 ± 21 s (60 ± 7 tetani); at the high intensity (duty cycle ∼0.3) force was reduced to 30 % in 42 ± 7 s (43 ± 7 tetani) (P < 0.05; n= 14). When force was reduced to 30 % of initial values, tetanic [Ca2+]i had fallen from 648 ± 87 to 336 ± 64 nm (48 % decrease) at the low intensity but had only fallen from 722 ± 84 to 468 ± 60 nm (35 % decrease) at the higher intensity (P < 0.05 low vs. high intensity; n= 7). Fatigue resulted in reductions in Ca2+ sensitivity of the contractile proteins which were greater at the high intensity (pre‐fatigue [Ca2+]i required for 50 % of maximum force (Ca50) = 354 ± 23 nm; post‐fatigue Ca50= 421 ± 48 nm and 524 ± 43 nm for low and high intensities, respectively). Reductions in maximum Ca2+‐activated force (Fmax) were similar at the two intensities (pre‐fatigue Fmax= 328 ± 22 μN; post‐fatigue Fmax= 271 ± 20 and 265 ± 19 μN for low and high intensities, respectively). Resting pHi was 7.15 ± 0.05. During fatigue at the low intensity, pHi was reduced by 0.12 ± 0.02 pH units and at the high intensity pHi was reduced by 0.34 ± 0.07 pH units (P < 0.05; n= 5). Our results indicate that the more rapid fall in force at a high intensity is due to a reduction in Ca2+ sensitivity of the contractile proteins, probably related to the greater acidosis. Our data also indicate that the failure of Ca2+ release and reduced maximum Ca2+‐activated force observed during fatigue are not due to reductions in intracellular pH.

Effects of Low-Level Laser Therapy (LLLT) in the Development of Exercise-Induced Skeletal Muscle Fatigue and Changes in Biochemical Markers Related to Postexercise Recovery

“…In addition, some studies have indicated that high H + concentrations can inhibit the linking of Ca +2 to troponin and thereby inhibit the interaction between contractile proteins. 10 Dose and treatment procedure seem to be important to achieve positive effects of LLLT in muscle tissue. In previous studies we had irradiated the biceps muscle at 4 locations using a single-diode laser but failed to find significant differences in blood lactate levels compared to controls.…”

Skeletal Muscle Fatigue: Cellular Mechanisms

“…However, this acidosis is not an invariable accompaniment of muscle activity and presumably depends on the extent to which anaerobic glycolysis is switched on and the rate at which protons leave the muscle on the lactate transporter or the Na ϩ /H ϩ exchanger. Typically after long-lasting activity, particularly at a low duty cycle, there is little or no acidosis (91,452) (see sect. VIIB).…”

Properties of single FDB fibers following a collagenase digestion for studying contractility, fatigue, and pCa-sarcomere shortening relationship

“…Many studies on muscle fatigue using mechanically dissected single FDB fibers have been carried out at room temperature (15)(16)(17). In one study carried out at 37°C (37), tetanic contractions were 400 ms long, and under those conditions, fibers stopped contracting within 1.5 s because of a large ROS production.…”