The contribution of oxidative stress to platelet senescence during storage

Wang, Li; Xie, Ruiqin; Fan, Zhijia; Yang, Jie; Liang, Wei; Wu, Qiang; Wu, Minxian; Wang, Zhicheng; Lu, Yuan

doi:10.1111/trf.15291

Cited by 12 publications

(8 citation statements)

References 55 publications

(68 reference statements)

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“…On the contrary, regulations of hemostasis, cell division, and biosynthetic processes were reduced.For example, the growth factor adapter protein (p66Shc), a 66-kDa isoform of ShcA, was reported a rolein ROS generation, which resulting in the oxidative stress during platelet storage. Our proteomic data show that p66Shc expressions increased on the later phase of platelet storage, which gave us inspiration to probe the mechanisms under platelet storage lesions in our recently published paper [55].…”

Section: Discussionmentioning

confidence: 56%

The Effects of Storage on Platelet Proteome Assessed by Protein Expression Changes and Lysine Modification Changes Using Tmt Isobaric Labeling

Wang

Yan

Fan

et al. 2020

Preprint

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Purpose:To provide the first insight into the proteomic dynamics during platelet storage.Experiment design: In this study, based on TMT-labeled LC-MS/MS analysis, combined with antibody-affinity enrichment and purification for acetylated and succinylated peptides, we performed quantification of global proteomics, acetylome and succinylome.Simultaneously, dynamic molecular changes and functional transformation of platelet were also characterized under proper conditions stored for 1, 3, 5, 7 days, respectively.Results:3,100 proteins are quantified from a total of 3,609 proteins identified from platelets. Out of 1,308 acetylated sites identified in 648 proteins, 790 sites in 396 proteinsare quantifiable. There are 1,947 succinylated sites in 959 proteins in which 1,279 sites in 661 proteins are quantifiable.We screened the differential expression changes of global proteins, acetyl- and succinyl- proteins, and systematically interpreted their molecular functions, biological processes, cellular components, pathways and motif characters to fully investigate the molecular dynamics and biological functions of platelets. Conclusions and clinical relevance:This paper is the first systematic exploration of proteomes and modified proteomes of platelet dynamics during storage in the aim to improve our understanding of platelet biology, which may be a valuable reference for further research and clinical application.

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Section: Discussionmentioning

confidence: 56%

The Effects of Storage on Platelet Proteome Assessed by Protein Expression Changes and Lysine Modification Changes Using Tmt Isobaric Labeling

Wang

Yan

Fan

et al. 2020

Preprint

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“…Resveratrol is a food-sourced antioxidant, with high safety even at a large dose and is rapidly metabolized by the liver in vivo. , It has been demonstrated to be able to alleviate PSL and prolong platelet storage time. , We thus developed a PEGylated liposomal resveratrol (Res-Lipo) as a protectant for platelet storage. Res-Lipo efficiently ameliorated ROS generation and ROS-induced PSL, with a much higher efficacy in comparison with free Res.…”

Section: Discussionmentioning

confidence: 99%

“…26,27 Res exhibited an effect on alleviating PSL by exerting their antioxidant capacity as N-acetyl-L-cysteine (a clinically used antioxidant) did. 26 However, the clinical application of Res is limited due to its low water solubility (0.03 g/L) and poor stability. 28 An effective delivery system for Res can be helpful for improving its therapeutic effects.…”

Section: ■ Introductionmentioning

confidence: 99%

Liposomal Resveratrol Alleviates Platelet Storage Lesion via Antioxidation and the Physical Buffering Effect

Hou,

Xiong,

Shao

et al. 2023

ACS Appl. Mater. Interfaces

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Platelet transfusion is essential in the treatment of platelet-related diseases and the prevention of bleeding in patients with surgical procedures. Platelet transfusion efficacy and shelf life are limited mainly by the development of platelet storage lesion (PSL). Mitigating PSL is the key to prolonging the platelet shelf life and reducing wastage. Excess intracellular reactive oxygen species (ROS) are one of the main factors causing PSL. In this study, we explored a nanomedicine strategy to improve the quality and functions of platelets in storage. Resveratrol (Res), a natural plant product, is known for its antioxidative effect. However, medical applications of Res are limited due to its low water solubility and stability. Therefore, we used a resveratrol-loaded liposomal system (Res-Lipo) to better utilize the antioxidant effect of the drug. This study aimed to evaluate the effect of Res-Lipo on platelet oxidative stress and alleviation of PSL during the storage time. Res-Lipo scavenged intracellular ROS and inhibited platelet apoptosis and activation during storage. Res-Lipo not only maintained mitochondrial function but also improved platelet aggregation in response to adenosine 5′-diphosphate. These results revealed that Res-Lipo ameliorated PSL and prolonged the platelet survival time in vivo. The strategy provides a potential method for extending the platelet storage time and might be considered a potential and safe additive to alleviate PSL.

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“… 18 Consistent with the generation of a senescent phenotype during platelet storage, research has shown that there is a significant increase in platelets positive for senescent-associated β-galactosidase after 3 days of storage, with almost 90% positivity by day 5. 22 Furthermore, platelets taking on a senescent phenotype in transfusion bags have been linked with the development of platelet storage lesions. In these circumstances, it has been demonstrated that there are extensive changes in the proteome of platelet-derived extracellular vesicles which may contribute to adverse outcomes following transfusion.…”

Section: Can Platelets Become Senescent?mentioning

confidence: 99%

What can we learn from senescent platelets, their transcriptomes and proteomes?

et al. 2023

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Research into the natural aging process of platelets has garnered much research interest in recent years, and there have long been associations drawn between the proportion of newly formed platelets in the circulation and the risk of thrombosis. However, these observations have largely been demonstrated in patient groups in which there may be underlying systemic changes that effect platelet function. Recent advances in technology have allowed in-depth analysis of differently aged platelets isolated from the peripheral blood of healthy individuals and have demonstrated that aged platelets, often referred to as senescent platelets, undergo extensive changes in the transcriptome and proteome. Ultimately, these changes result in platelets whose functions have deteriorated such that they cannot partake in hemostatic responses to the same extent as newly formed platelets. Here, we review transcriptomic and proteomic research in platelet aging in the context of health and how this research sheds light upon alterations in platelet structure and function.

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The contribution of oxidative stress to platelet senescence during storage

Abstract: BACKGROUND: Platelets for transfusion become senescent and dysfunctional during storage, resulting in a markedly short shelf life (5 days). We hypothesized that oxidative stress might account for this decline.From the

Cited by 12 publications

References 55 publications

The Effects of Storage on Platelet Proteome Assessed by Protein Expression Changes and Lysine Modification Changes Using Tmt Isobaric Labeling

The Effects of Storage on Platelet Proteome Assessed by Protein Expression Changes and Lysine Modification Changes Using Tmt Isobaric Labeling

Liposomal Resveratrol Alleviates Platelet Storage Lesion via Antioxidation and the Physical Buffering Effect

What can we learn from senescent platelets, their transcriptomes and proteomes?

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