The continuous isolation of trypsin by affinity adsorbent recycling

Gill, Alison L.; Niven, Gordon W.; Scurlock, Peter G.

doi:10.1002/(sici)1097-0290(19971205)56:5<538::aid-bit7>3.0.co;2-j

Cited by 3 publications

(2 citation statements)

References 21 publications

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“…The conventional immobilized ligands used for purification of trypsin are immobilized metal ion, dye ligands, and so on [35,36]. Compared with these conventional ligands, the multi modal small ligands will be more attractive due to their specific interaction with target protein, freedom from toxicity, and low cost [37,38].…”

Section: Qcm Sensor For Screening Of Interactions Between Ligands Andmentioning

confidence: 99%

Development of a sensitive method for selection of affinity ligand for trypsin using quartz crystal microbalance sensor

Bayramoğlu

Arıca

2011

Bioprocess Biosyst Eng

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In this work, a new methodology is developed for selection of affinity ligands towards the enzyme "trypsin" using quartz crystals microbalance (QCM) technique. To achieve this goal, the surface amination of gold plated QCM crystals was achieved in 13.56 MHz plasma polymerization system by using ethylenediamine. Three different ligands (i.e., 4-aminobenzamidine, 4-aminobenzoic acid, and phenylalanine) were immobilized on the aminated QCM crystals surface via glutaraldehyde coupling. All three ligand immobilized QCM crystals were characterized and compared under different experimental conditions. It was observed that the benzamidine ligand showed higher affinity to trypsin with a dissociation constant on the order of 1.76 × 10(-9) M, which is within the range of 10(-4)-10(-8) M for affinity ligands. Thus, its selectivity was suitable for purification of trypsin from biological fluids.

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Section: Qcm Sensor For Screening Of Interactions Between Ligands Andmentioning

confidence: 99%

Development of a sensitive method for selection of affinity ligand for trypsin using quartz crystal microbalance sensor

Bayramoğlu

Arıca

2011

Bioprocess Biosyst Eng

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“…Dewatering and lysis components in design 3 were identical to design 2. Protein purification was based on a non-commercial affinity belt system [57][58][59] . Rollers were proposed to move an affinity membrane belt continuously through chambers containing the lysate, wash buffers, and elution buffers.…”

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confidence: 99%

Theoretical design of a space bioprocessing system to produce recombinant proteins

Soundararajan

Paddock

Dougherty

et al. 2022

Preprint

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Space-based biomanufacturing has the potential to improve the sustainability of deep space exploration. To advance biomanufacturing, bioprocessing systems need to be developed for space applications. Here, commercial technologies were assessed to design space bioprocessing systems to supply a liquid amine carbon dioxide scrubber with active carbonic anhydrase produced recombinantly. Design workflows encompassed biomass dewatering of 1 L Escherichia coli cultures through to recombinant protein purification. Equivalent system mass (ESM) analyses had limited utility for selecting specific technologies. Instead, bioprocessing system designs focused on minimizing complexity and enabling system versatility. Three designs that differed in biomass dewatering and protein purification approaches had nearly equivalent ESM of 357-522 kg eq. Values from the system complexity metric (SCM), technology readiness level (TRL), and degree of crew assistance metric identified a simpler, less costly, and easier to operate design for automated biomass dewatering, cell lysis, and protein affinity purification.

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Enzyme immobilization on nylon–optimization and the steps used to prevent enzyme leakage from the support

Isgrove

Williams

Niven

et al. 2001

Enzyme and Microbial Technology

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The continuous isolation of trypsin by affinity adsorbent recycling

Cited by 3 publications

References 21 publications

Development of a sensitive method for selection of affinity ligand for trypsin using quartz crystal microbalance sensor

Development of a sensitive method for selection of affinity ligand for trypsin using quartz crystal microbalance sensor

Theoretical design of a space bioprocessing system to produce recombinant proteins

Enzyme immobilization on nylon–optimization and the steps used to prevent enzyme leakage from the support

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