The content of 5-methylcytosine in animal DNA: The species and tissue specificity

Vanyushin, B. F.; Mazin, A L; Vasilyev, V.K.; Belozersky, A. N.

doi:10.1016/0005-2787(73)90264-5

Cited by 147 publications

(51 citation statements)

References 14 publications

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“…In particular, tissue-specific differences in methylation patterns have been observed (Vanyushin et al, 1973), namely for the globin gene (Waalwijk & FlaveU, 1978 b;McGhee & Ginder, 1979;, for chicken ovalbumin (Mandel & Chambon, 1979;Kuo et al, 1979) and for a variety of genes in the sea urchin Echinus esculentus (Bird et al, 1979). In most cases, undermethylated genes have been observed to be more actively expressed.…”

Section: (B) Non-virus Eukaryotic Systemsmentioning

confidence: 99%

DNA Methylation--A Regulatory Signal in Eukaryotic Gene Expression

Doerfler

1981

Journal of General Virology

184

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Section: (B) Non-virus Eukaryotic Systemsmentioning

confidence: 99%

DNA Methylation--A Regulatory Signal in Eukaryotic Gene Expression

Doerfler

1981

Journal of General Virology

184

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“…This finding suggests that autosomal imprinting, possibly maintained by differential DNA methylation, may be related to the mechanism by which a single member of a pair of X chromosomes is inactivated and maintained in the silent, late-replicating state (25). While genetic imprinting has not been investigated in P. polycephalum, it is known that 4 to 8% of the cytosines in the nuclear DNA are methylated (17), compared with about 3% in vertebrates (69). The allele-specific replication schedule of the altA locus suggests that this may be an appropriate region of the genome in which to search for differences in DNA sequence organization, chromatin conformation, or nucleotide modification that may distinguish homologous autosomes.…”

Section: Discussionmentioning

confidence: 99%

Two alleles of a developmentally regulated alpha-tubulin locus in Physarum polycephalum replicate on different schedules.

Cunningham

Dove

1993

Mol. Cell. Biol.

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The replication timing of a pair of natural alleles was compared at two ac-tubulin loci of the Physarum plasmodium. Taking advantage of the naturally synchronous cell cycle of nuclei within the syncytial plasmodium, we analyzed the replication schedule of specific DNA fragments to a resolution of 10-min intervals within a 3-h S phase. At this level of resolution, differences in replication timing between polymorphic alleles at the same locus can be detected in a heterozygote. Specifically, the 3' region of the altAl allele completes replication at between 20 and 40 min of S phase. The same region of the a4tA2 allele completes replication at between 40 and 80 min of S phase. In contrast, both alleles at the altB locus replicate concurrently within the first 10 to 15 min of S phase. Previous studies showed that both aWl and altB are expressed in the plasmodium, their message levels peaking at mitosis, just minutes before the onset of S phase. However, altB message is detected at substantially higher levels than alt4 message on Northern (RNA) blots. The A recurring theme within the variety of recent advances in the investigation of eukaryotic DNA replication is that chromosomal context is a fundamental determinant of origin activity (20,23,44,64). It is reasonable to assume that not only origin activity but also replication fork progression and termination are sensitive to the chromosomal context. This position effect may be a reflection of the longitudinal heterogeneity of a chromosome with regard to protein composition, nucleotide base composition, transcriptional activity, higher-order structure, and association with the nuclear matrix. With this in mind, we have sought to analyze

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“…The therapeutic effect of exposure was evaluated by inhibition of tumor growth. To determine the level of DNA-methylation, DNA was extracted, after the slaughtering of the animals, from tumor cells by phenol-chloroform method in the presence of 1.5%-SDS [21]. Hydrolysis to the nitrogen bases were carried out in the sealed glass ampoules in 85%-formic acid at 176 • C for one hour (0.1 mL of acid per 1 mg of DNA).…”

Section: Methodsmentioning

confidence: 99%

Effect on Tumoral Cells of Low Intensity Electromagnetic Waves

Kalantaryan¹,

Martirosyan²,

Nersesyan³

et al. 2011

PIER Letters

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Abstract-Violations of the process of methylation reveal itself at the early stages of malignant transformation of cells, and the content of 5-methylcytosine can serve as a diagnostic test for tumor formation and treatment of disease. The carried out studies revealed the correlation of antitumor activity of MM-therapy (the use of coherent millimeter electromagnetic waves of low intensity for therapy) with inhibition of methylation of tumor DNA in vivo. It is revealed in our experiments that an half-hour exposure of MM-radiation results in the tumor growth inhibition by 33.5% and a sharp suppression of the level of DNA-methylation 2.5 times. The results obtained in this experiment indicate the prospects of working out the MM-therapy for clinical oncology in the treatment of malignant neoplasms.

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The content of 5-methylcytosine in animal DNA: The species and tissue specificity

Cited by 147 publications

References 14 publications

DNA Methylation--A Regulatory Signal in Eukaryotic Gene Expression

DNA Methylation--A Regulatory Signal in Eukaryotic Gene Expression

Two alleles of a developmentally regulated alpha-tubulin locus in Physarum polycephalum replicate on different schedules.

Effect on Tumoral Cells of Low Intensity Electromagnetic Waves

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