The construction of the World Wide Web‐accessible myocardial two‐dimensional gel electrophoresis protein database “HEART‐2DPAGE”: A practical approach

Pleißner, Klaus‐Peter; Sander, Stefan; Oswald, H. R.; Regitz‐Zagrosek, Vera; Fleck, Eckart

doi:10.1002/elps.1150170818

Cited by 44 publications

(10 citation statements)

References 17 publications

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“…As mentioned earlier, the protein database will be important to serve as a basis for further studies of human disease. There are four 2-DE protein databases of cardiac proteins, established by three independent groups, which can be accessed via the World Wide Web [7,[14][15][16] . These databases facilitate proteomic research into heart diseases containing information In addition, 2-DE protein databases and proteomic maps for other mammals are also under construction to support work on animal models of heart disease [17][18][19][20] .…”

Section: Discussionmentioning

confidence: 99%

Proteome reference map and regulation network of neonatal rat cardiomyocyte

Liu

Han

et al. 2011

Acta Pharmacol Sin

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Aim: To study and establish a proteome reference map and regulation network of neonatal rat cardiomyocyte. Methods: Cultured cardiomyocytes of neonatal rats were used. All proteins expressed in the cardiomyocytes were separated and identified by two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). Biological networks and pathways of the neonatal rat cardiomyocytes were analyzed using the Ingenuity Pathway Analysis (IPA) program (www.ingenuity.com). A 2-DE database was made accessible on-line by Make2ddb package on a web server. Results: More than 1000 proteins were separated on 2D gels, and 148 proteins were identified. The identified proteins were used for the construction of an extensible markup language-based database. Biological networks and pathways were constructed to analyze the functions associate with cardiomyocyte proteins in the database. The 2-DE database of rat cardiomyocyte proteins can be accessed at http://2d.bjmu.edu.cn. Conclusion: A proteome reference map and regulation network of the neonatal rat cardiomyocytes have been established, which may serve as an international platform for storage, analysis and visualization of cardiomyocyte proteomic data.

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Section: Discussionmentioning

confidence: 99%

Proteome reference map and regulation network of neonatal rat cardiomyocyte

Liu

Han

et al. 2011

Acta Pharmacol Sin

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“…In this study we present further data which have been incorporated into the myocardial protein database described previously [20]. We analyzed the disease-specific pattern of protein expression in 13 patients with dilated cardiomyopathy compared to 15 control patients.…”

Section: Discussionmentioning

confidence: 99%

“…Since the existing studies included only data from a relatively small number of patients and therefore were limited from a statistical point of view, we here report the establishment of a large study comparing the myocardial protein patterns in patients with dilated cardiomyopathy with the protein patterns in a control group. The data have been added to the database previously described by us [20] and are accessible via the Internet (http://www.chemie.fuberlin.de/user/pleiss).…”

Section: Introductionmentioning

confidence: 99%

Dilated cardiomyopathy‐associated proteins and their presentation in a WWW‐accessible two‐dimensional gel protein database

et al. 1997

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High resolution two-dimensional electrophoresis (2-DE) and computer-assisted image analysis were used to screen 13 patients suffering from dilated cardiomyopathy (DCM) versus 15 control patients for quantitative and qualitative differences in their myocardial protein expression. Right atrial tissue samples were obtained from end-stage failing explanted hearts and control hearts. Fifty-two spots differed significantly in average intensity between the DCM and the control groups. Myosin light chain 2, ventricular (MLC2) and heat shock protein HSP 27 were identified by protein microsequencing and gel map comparison with other databases. These proteins were found to be characteristic protein markers for DCM in the right atrium. In DCM patients, the spot intensity (protein abundance) of MLC2 is increased to 336% and HSP 27 is decreased to 59%, compared to the control group. The HEART-2DPAGE, a World Wide Web-accessible 2-DE database, was used and extended for the presentation of these disease-associated proteins. Retrievable via Internet we present a list of disease-associated proteins, their altered level of expression in DCM, and their position on a right atrial protein pattern. The accession number to protein sequence databases confers a connection to databases like SWISS-PROT to obtain a detailed functional and structural description of disease-associated proteins. New DCM-associated proteins are detected and their presentation in a 2-DE gel protein database is described.

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“…addition of sugar moieties or lipid attachments to a protein), and interaction with other biological molecules, thus providing new insights into the complex cellular processes involved in cardiovascular dysfunction. To this end, several human cardiac protein databases have been established, 81 and proteome analyses in various human cardiomyopathies 59 88 and animal models of heart disease 44 110 have been reported. The potential for`protein chips' to function as versatile and rigorous high-throughput methods for proteomic analyses is the object of intense investigation.…”

Section: Genome±proteome±physiome/cardiome± Phenomementioning

confidence: 99%