The Construction of Carbon Nanotubes Containing an Anti-Bacterial Chemical Component and its Effect on MDR and XDR Isolates of Pseudomonas Aeruginosa

Abstract

Background: Pseudomonas aeruginosa is an opportunistic human pathogen that causes severe acute and chronic nosocomial infections, especially in immunocompromised burn patients. and can lead to severe mortality and morbidity. The emergence of antibiotic resistant P. aeruginosa infections has created significant challenges in treating these patients. A potential alternative treatment for antibiotic resistant pathogens includes the use of carbon nanotubes (CNTs), which have received considerable attention due to their potent antibacterial activity. The aim of this study was to construct a novel CNT containing an anti-bacterial chemical component to effectively combat drug resistant P. aeruginosa infections. Methods: In this study, a novel chemical component was synthesized and coated the CNT. The antimicrobial effects were then evaluated on MDR, XDR, and PDR strains of P. aeruginosa isolated from burn patients. Antibiotic susceptibility was evaluated using the disk diffusion test and minimum inhibitory concentration (MIC) testing. In order to determine the potential cytotoxicity, an MTT assay was performed on Human Dermal Fibroblasts. The effect of treatment on the expression of wound healing genes was analyzed via qRT-PCR. Results: Experimental data indicates that our CNT coated chemical compound had antibacterial properties, negligible cytotoxicity, and could accelerate the wound healing process. Conclusions: Given the antibacterial properties of our CNT chemical compound, it has the potential to treat and reduce the occurrence of multi-drug resistant P. aeruginosa burn wound infections and aid in wound healing by turning on genes (VEGFA, EGF and PDEGF) involved in the wound healing process.

Molecular Investigation of Outer Membrane Channel Genes Among Multidrug Resistance Clinical Pseudomonas Aeruginosa Isolates

“…This may have different consequences on the outcome of infection (42). So, our result revealed that the isolate has high virulence mechanism to deliver the effector proteins affecting host cells and other bacteria in their milieu (43)(44)(45).…”

Antibacterial effect of carbon nanotube containing chemical compounds on drug-resistant isolates of Acinetobacter baumannii

“…Banihashemi et al in 2020 investigated the antibacterial properties of this same chemical compounds coated onto the same carbon nanotubes on 30 burned Wistar mice which were artificially infected with Pseudomonas aeruginosa . They concluded that simultaneous treatment of the compound and nanotubes was highly effective for treatment ( 37 ). Limitation of the current study was that we have not yet tested the chemical compound directly on human burn wounds.…”

Recent advances in nanoantibiotics against multidrug-resistant bacteria

“…Researchers created Ag@CNT/PA composite nanobers by combining polyamide (PA), CNTs, and Ag NPs, which exhibited remarkable antibacterial properties and effectively combated drug resistance. 202 The typical 2D carbon materials including graphene and MXene have also demonstrated good antibacterial activity. Graphene has garnered extensive attention due to its high antibacterial activity with little resistance and high biocompatibility, which is an ideal carrier for antibacterial substances.…”

“…Researchers created Ag@CNT/PA composite nanofibers by combining polyamide (PA), CNTs, and Ag NPs, which exhibited remarkable antibacterial properties and effectively combated drug resistance. 202…”