The construction of an EST database for
            <i>Bombyx mori</i>
            and its application

Mita, Kazuei; Morimyo, Mitsuoki; Okano, Kazuhiro; Koike, Yoko; Nohata, Junko; Kawasaki, Hideki; Kadono-Okuda, Keiko; Yamamoto, Kimiko; Suzuki, Masataka; Shimada, Toru; Goldsmith, Marian R.; Maeda, Susumu

doi:10.1073/pnas.2234984100

Cited by 249 publications

(219 citation statements)

References 51 publications

(37 reference statements)

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“…In comparison, EST analysis is not dependent on prior knowledge of the whole genome, but relies on identification of the transcripts by homology. A careful analysis of ESTs from various tissues of the silkworm (Mita et al, 2003) indicates that about one-third or less of the ESTs found were specific for each tissuederived library. In contrast, the silkworm prothoracic gland library yielded 49% library-specific ESTs, but a detailed description and analysis is not available.…”

Section: Discussionmentioning

confidence: 99%

Comparative genomics of insect juvenile hormone biosynthesis☆

Noriega

Ribeiro

Koener

et al. 2006

Insect Biochemistry and Molecular Biology

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The biosynthesis of insect juvenile hormone (JH) and its neuroendocrine control are attractive targets for chemical control of insect pests and vectors of disease. To facilitate the molecular study of JH biosynthesis, we analyzed ESTs from the glands producing JH, the corpora allata (CA) in the cockroach Diploptera punctata, an insect long used as a physiological model species and compared them with ESTs from the CA of the mosquitoes Aedes aegypti and Anopheles albimanus. The predicted genes were analyzed according to their probable functions with the Gene Ontology classification, and compared to Drosophila and Anopheles gambiae genes. A large number of reciprocal matches in the cDNA libraries of cockroach and mosquito CA were found. These matches defined known and suspected enzymes of the JH biosynthetic pathway, but also several proteins associated with signal transduction that might play a role in the modulation of JH synthesis by neuropeptides. The identification in both cockroach and mosquito CA of homologs of the small ligand binding proteins from insects, Takeout/JH binding protein and retinol-binding protein highlights a hitherto unsuspected complexity of metabolite trafficking, perhaps JH precursor trafficking, in these endocrine glands. Furthermore, many reciprocal matches for genes of unknown function may provide a fertile ground for an in-depth study of allatal-specific cell physiology.

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Section: Discussionmentioning

confidence: 99%

Comparative genomics of insect juvenile hormone biosynthesis☆

Noriega

Ribeiro

Koener

et al. 2006

Insect Biochemistry and Molecular Biology

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“…Most of the research, both pure and applied, on these silks has been carried out on silks made by Lepidoptera (silkworms), because by far the largest quantity of silk comes from the silkworm Bombyx mori. [1][2][3][4][5] The amino acid composition and sequence of B. mori silk are well known from genetic analyses. 4) B. mori silk is composed of fiber protein (fibroin) and a coating glue protein (sericin).…”

mentioning

confidence: 99%

“…[1][2][3][4][5] The amino acid composition and sequence of B. mori silk are well known from genetic analyses. 4) B. mori silk is composed of fiber protein (fibroin) and a coating glue protein (sericin). 5) The fibroin and sericin are organized in higher order repeats of alternations or strings of small amino acids such as glycine (Gly), alanine (Ala), and serine (Ser) residues.…”

mentioning

confidence: 99%

Identification of Four Major Hornet Silk Genes with a Complex of Alanine-Rich and Serine-Rich Sequences inVespa simillima xanthopteraCameron

Sezutsu¹,

Kajiwara²,

Kojima³

et al. 2007

Bioscience, Biotechnology, and Biochemistry

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“…Within this area, several highly conserved motifs, such as IFAYGQT and LVDLAGSE, are present across species and are considered as sequence markers of kinesins [30]. Here, we scanned the silkworm genome [21] and EST database [22] to look for these sequence markers, and successfully located five homologous sequences (Supplementary information, Figure S1A). We extended the conserved sequences forward and backward (Supplementary information, Figure S1B), either by applying Basic Local Alignment Search Tool (BLAST) in silkworm cDNA and genome database or by comparing sequences between Dm and Bm.…”

Section: Identification Of Klps In Silkworm Silkglandmentioning

confidence: 99%

“…Their large-sized PSGs [19] are relatively easy to dissect for biochemical analysis, such as protein purification and pull-down analysis. Silkworm genomic sequences and cDNA databases are available [21,22]; hence, the cDNA cloning of kinesins and their associated proteins is no longer difficult. Most importantly, PSG is a highly differentiated secretory tissue, with a complex and extensive cytoskeleton system and endomembrane system (Golgi apparatus, ER, and secretory granules) occupying a large portion of the cytoplasm [19,23].…”

Section: Introductionmentioning

confidence: 99%