The construction and immunogenicity analyses of a recombinant pseudorabies virus with porcine circovirus type 3 capsid protein co-expression

Yao, Lun; Cheng, Yufang; Wu, Hao; Ghonaim, Ahmed H.; Su, Fan; Li, Wentao; He, Qigai

doi:10.1016/j.vetmic.2021.109283

Cited by 6 publications

(3 citation statements)

References 31 publications

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“…In other studies, researchers inserted the PCV2 ORF2 or PCV3 ORF2 genes into an adenovirus vector to construct a live vector vaccine. After immunization, specific antibodies against PCV2 or PCV3 were significantly higher than in the control group, indicating a robust humoral immune response ( 20 , 34 ), consistent with our findings, where Cap protein induced effective humoral immune responses when immunizing mice using various live vectors.…”

Section: Discussionsupporting

confidence: 91%

Construction of recombinant pseudorabies virus expressing PCV2 Cap, PCV3 Cap, and IL-4: investigation of their biological characteristics and immunogenicity

Yang,

Xu,

Tao

et al. 2024

Front. Immunol.

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BackgroundPorcine circovirus type 2 (PCV2) is a globally prevalent and recurrent pathogen that primarily causes slow growth and immunosuppression in pigs. Porcine circovirus type 3 (PCV3), a recently discovered virus, commonly leads to reproductive disorders in pigs and has been extensively disseminated worldwide. Infection with a single PCV subtype alone does not induce severe porcine circovirus-associated diseases (PCVD), whereas concurrent co-infection with PCV2 and PCV3 exacerbates the clinical manifestations. Pseudorabies (PR), a highly contagious disease in pigs, pose a significant threat to the swine industry in China.MethodsIn this study, recombinant strains named rPRV-2Cap/3Cap and rPRV-2Cap/3Cap/IL4 was constructed by using a variant strain XJ of pseudorabies virus (PRV) as the parental strain, with the TK/gE/gI genes deleted and simultaneous expression of PCV2 Cap, PCV3 Cap, and IL-4. The two recombinant strains obtained by CRISPR/Cas gE gene editing technology and homologous recombination technology has genetic stability in baby hamster Syrian kidney-21 (BHK-21) cells and is safe to mice.ResultsrPRV-2Cap/3Cap and rPRV-2Cap/3Cap/IL4 exhibited good safety and immunogenicity in mice, inducing high levels of antibodies, demonstrated 100% protection against the PRV challenge in mice, reduced viral loads and mitigated pathological changes in the heart, lungs, spleen, and lymph nodes during PCV2 challenge. Moreover, the recombinant viruses with the addition of IL-4 as a molecular adjuvant outperformed the non-addition group in most indicators.ConclusionrPRV-2Cap/3Cap and rPRV-2Cap/3Cap/IL4 hold promise as recombinant vaccines for the simultaneous prevention of PCV2, PCV3, and PRV, while IL-4, as a vaccine molecular adjuvant, effectively enhances the immune response of the vaccine.

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Section: Discussionsupporting

confidence: 91%

Construction of recombinant pseudorabies virus expressing PCV2 Cap, PCV3 Cap, and IL-4: investigation of their biological characteristics and immunogenicity

Yang,

Xu,

Tao

et al. 2024

Front. Immunol.

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“…Due to its high capacity, PRV has become a powerful vector system for expressing exogenous proteins. It can express the main immunogenic protein of other pathogens, such as the Cap protein of porcine circovirus (PCV) ( 17 ), VP2 of porcine parvovirus (PPV) ( 18 ), CD2v of African swine fever virus (ASFV) ( 19 ), S of porcine deltacoronavirus (PDCoV) ( 20 ), and GP5 and M of porcine reproductive and respiratory syndrome virus (PRRSV) ( 21 ).…”

Section: Introductionmentioning

confidence: 99%

The Construction and Immunogenicity Analyses of a Recombinant Pseudorabies Virus with Senecavirus A VP2 Protein Coexpression

Tao

Zhu

et al. 2023

Microbiol Spectr

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“…The absence of these nonessential genes results in a diminished virulence phenotype of the virus while having no significant effect on the immune response ( Klupp et al, 2004 ; Olsen et al, 2006 ). At present, PRV has developed into a powerful vector system for expressing exogenous protein, which can carry and express the main immunogenic genes and protein of other viruses, such as PCV2 Cap, PCV3 Cap, PPV VP2, ASFV CD2v, HP-PRRSV GP5, and M proteins ( Freuling et al, 2017 ; Feng et al, 2020 ; Zheng et al, 2020 ; Yao et al, 2021 ). These bivalent or trivalent recombinant vaccines developed with a PRV live virus vector can stimulate the body to produce a specific immune response to the target virus without affecting the autogenous immune effect of PRV.…”

Section: Introductionmentioning

confidence: 99%

The Construction and Immunogenicity Analyses of Recombinant Pseudorabies Virus With NADC30-Like Porcine Reproductive and Respiratory Syndrome Virus-Like Particles Co-expression

Zhao

Zhu

et al. 2022

Front. Microbiol.

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Porcine reproductive and respiratory syndrome (PRRS) and pseudorabies (PR) are highly infectious swine diseases and cause significant financial loss in China. The respiratory system and reproductive system are the main target systems. Previous studies showed that the existing PR virus (PRV) and PRRS virus (PRRSV) commercial vaccines could not provide complete protection against PRV variant strains and NADC30-like PRRSV strains in China. In this study, the PRV variant strain XJ and NADC30-like PRRSV strain CHSCDJY-2019 are used as the parent for constructing a recombinant pseudorabies virus (rPRV)-NC56 with gE/gI/TK gene deletion and co-expressing NADC30-like PRRSV GP5 and M protein. The rPRV-NC56 proliferated stably in BHK-21 cells, and it could stably express GP5 and M protein. Due to the introduction of the self-cleaving 2A peptide, GP5 and M protein were able to express independently and form virus-like particles (VLPs) of PRRSV in rPRV-NC56-infected BHK-21 cells. The rPRV-NC56 is safe for use in mice; it can colonize and express the target protein in mouse lungs for a long time. Vaccination with rPRV-NC56 induces PRV and NADC30-like PRRSV specific humoral and cellular immune responses in mice, and protects 100% of mice from virulent PRV XJ strain. Furthermore, the virus-neutralizing antibody (VNA) elicited by rPRV-NC56 showed significantly lower titer against SCNJ-2016 (HP-PRRSV) than that against CHSCDJY-2019 (NADC30-like PRRSV). Thus, rPRV-NC56 appears to be a promising candidate vaccine against NADC30-like PRRSV and PRV for the control and eradication of the variant PRV and NADC30-like PRRSV.

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The construction and immunogenicity analyses of a recombinant pseudorabies virus with porcine circovirus type 3 capsid protein co-expression

Cited by 6 publications

References 31 publications

Construction of recombinant pseudorabies virus expressing PCV2 Cap, PCV3 Cap, and IL-4: investigation of their biological characteristics and immunogenicity

Construction of recombinant pseudorabies virus expressing PCV2 Cap, PCV3 Cap, and IL-4: investigation of their biological characteristics and immunogenicity

The Construction and Immunogenicity Analyses of a Recombinant Pseudorabies Virus with Senecavirus A VP2 Protein Coexpression

The Construction and Immunogenicity Analyses of Recombinant Pseudorabies Virus With NADC30-Like Porcine Reproductive and Respiratory Syndrome Virus-Like Particles Co-expression

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