The Construction and Analysis of lncRNA–miRNA–mRNA Competing Endogenous RNA Network of Schwann Cells in Diabetic Peripheral Neuropathy

Cheng, Wang; Xu, Xiang; Chen, Jing; Kang, Yu; Guo, Jiahe; Duscher, Dominik; Yang, Xiaofan; Guo, Guojun; Ren, Sen; Xiong, Hairong; Yuan, Meng; Jiang, Tao; Machens, Hans‐Günther; Chen, Zhenbing; Chen, Yanhua

doi:10.3389/fbioe.2020.00490

Cited by 21 publications

(21 citation statements)

References 68 publications

(78 reference statements)

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“…Previous studies have reported that the ceRNA mechanism can be used for the analysis of multiple diseases ( 36 , 57 ). Qu et al ( 36 ) constructed a miRNA-lncRNA-mRNA ceRNA regulatory network on the basis of DEGs and lncRNAs in patients with IDD.…”

Section: Discussionmentioning

confidence: 99%

“…Qu et al ( 36 ) constructed a miRNA-lncRNA-mRNA ceRNA regulatory network on the basis of DEGs and lncRNAs in patients with IDD. In addition, Wang et al ( 57 ) identified a novel ceRNA network that provides a novel insight for investigating the underlying molecular mechanism of diabetic peripheral neuropathy. In the present study, ITGB8 was inhibited by increased expression of MREs resulting from decreased expression of lncRNAs in the ceRNA network.…”

Section: Discussionmentioning

confidence: 99%

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Comprehensive evaluation of differential long non‑coding RNA and gene expression in patients with cartilaginous endplate degeneration of cervical vertebra

Yuan

Jia

et al. 2020

Exp Ther Med

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Long non-coding RNAs (lncRNAs) are emerging as key regulators in gene expression; however, little is currently known regarding their role in cartilaginous endplate (CE) degeneration (CED) of cervical vertebra. The present study aimed to investigate the expression levels of lncRNAs and analyze their potential functions in CED of cervical vertebra in patients with cervical fracture and cervical spondylosis. Human competitive endogenous RNA (ceRNA) array was used to analyze lncRNA and mRNA expression levels in CE samples from patients with cervical fracture and cervical spondylosis, who received anterior cervical discectomy and fusion. Differentially expressed lncRNAs (DELs) or differentially expressed genes (DEGs) were identified and functionally analyzed, using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. An lncRNA-microRNA(miRNA)-mRNA ceRNA regulatory network was constructed based on the DELs and DEGs, and the ceRNA network was visualized using Cytoscape 3.7.2 software. In total, one downregulated mRNA, one upregulated miRNA and five downstream regulated lncRNAs were identified using reverse transcription-quantitative PCR in CED and healthy CE samples. A total of 369 lncRNAs and 246 mRNAs were identified as differentially expressed in CE. The GO and KEGG analyses demonstrated that the majority of GO and KEGG enrichments were associated with CED. Furthermore, a ceRNA network was established, including 168 putative miRNA response elements, 189 upregulated and 37 downregulated lncRNAs and 47 upregulated and 10dow regulated DEGs. The present study analyzed the function of DEGs in the ceRNA network and filtered out the same items as in DEG-function enrichment analysis. These results provide a new perspective for an improved understanding of ceRNA-mediated gene regulation in cervical spondylosis, and provide a novel theoretical basis for further studies on the function of lncRNA in cervical spondylosis. However, further experiments are required to validate the results of the present study.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Comprehensive evaluation of differential long non‑coding RNA and gene expression in patients with cartilaginous endplate degeneration of cervical vertebra

Yuan

Jia

et al. 2020

Exp Ther Med

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“…Although the incidence of TN was higher in female rats than male, we only used male SD rats to establish the TN animal model, to avoid the effect of estrogen on experimental results. We only focused on transcrip-tome level changes; however, noncoding RNAs have been found to play an important role, which were not involved in our experiment (31,32). Besides the previously mentioned, we also did not identify the DEGs at the protein level.…”

Section: Limitationsmentioning

confidence: 97%

Using RNA-Seq to Explore the Hub Genes in the Trigeminal Root Entry Zone of Rats by Compression Injury

et al. 2021

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BACKGROUND: Mechanical compression on the trigeminal root entry zone (TREZ) by microvascular is the main etiology of primary trigeminal neuralgia (TN). OBJECTIVES: To study the pathogenesis of TN, hub genes screening in the TREZ of TN in an animal model was performed. STUDY DESIGN: A double blind, randomized study was designed in a controlled animal trial. SETTING: The research took place in the Laboratory of Clinical Applied Anatomy at the School of Basic Medical Science of Fujian Medical University. METHODS: Twelve male rats were randomly divided into a sham operation group and a TN animal model group. TN animal model was induced by chronic compression of trigeminal nerve root (CCT) operation. Gene expression in the TREZ were analyzed by RNA sequencing (RNA-Seq) technique. KEGG analysis, GO analysis, and PPI analysis were performed in the DEGs. Key signaling pathways analyzing by GSEA and the hub genes in the DEGs were also studied. Reverse transcription real-time polymerase chain reaction (RT-qPCR) was used to verify the RNA-Seq results. RESULTS: Transcriptome data showed that 352 genes up-regulated and 59 genes down-regulated in DEGs on post-operation day 21, after CCT operation in the TN group. KEGG analysis revealed that, “neuroactive ligand receptor interaction” and “cytokine cytokine receptor interaction” may be related to the pathogenesis of TN. GO analysis showed “regulation of signing receptor activity”, “chemokine activity”, and “carbohydrate binging” may be related to TN. The RT-qPCR results were consistent with the test results, indicating that the transcriptome sequencing results were reliable. LIMITATIONS: Although the incidence of TN in female rats was higher than in male rats, we only used male SD rats to establish the TN animal model, to avoid the effect of estrogen on experimental results. This study only presents some respects of RNA-Seq technique and, therefore, did not identify the DEGs at the protein level. The relationship between the DEGs at different levels shoud be done in the future. CONCLUSIONS: Based on the results of RNA-seq, this study discovered 6 hub genes in the TREZ that are closely related to the TN animal model, which provide a potential breakthrough point to explore the pathogenesis of TN. KEY WORDS: Animal model, compression injury, hub gene, rat, RNA-seq, transcriptome, trigeminal neuralgia, trigeminal root entry zone

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“…Wang, C. et al employed whole-transcriptome sequencing technology to systematically analyze the differential expression of lncRNAs, mRNAs, and miRNAs in Schwann cells (SCs) of DPN and control rats and constructed lncRNA–miRNA–mRNA competing endogenous RNA (ceRNA) network of SCs. This network has identified the inhibited relationship of lncRNA, miRNA, and mRNA and underlined that they function as key mediators in the pathophysiological process of SCs in DPN ( Wang et al, 2020 ). This ceRNA regulatory network has a particular clinical application value in DPN.…”

Section: Regulation Of Lncrna On Nlrp3 Inflammation In Diabetes and Its Complicationsmentioning

confidence: 99%

Emerging Role of LncRNA Regulation for NLRP3 Inflammasome in Diabetes Complications

Lü

Tan

et al. 2022

Front. Cell Dev. Biol.

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Diabetes is a widespread metabolic disease with various complications, including diabetic nephropathy, retinopathy, cardiomyopathy, and other cardiovascular or cerebrovascular diseases. As the prevalence of diabetes increases in all age groups worldwide, diabetes and its complications cause an emerging public health burden. NLRP3 inflammasome is a complex of several proteins that play a critical role in inflammatory response and various diseases, including diabetes and its complications. Accumulating evidences indicate that NLRP3 inflammasome contributes to the development of diabetes and diabetic complications and that NLRP3 inflammation inactivation is beneficial in treating these illnesses. Emerging evidences suggest the critical role of long non-coding RNAs (lncRNAs) in regulating NLRP3 inflammasome activity in various diseases. LncRNAs are non-coding RNAs exceeding 200 nucleotides in length. Its dysregulation has been linked to the development of diseases, including diabetes. Recently, growing evidences hint that regulating lncRNAs on NLRP3 inflammasome is critical in developing and progressing diabetes and diabetic complications. Here, we discuss the role of lncRNAs in regulating NLRP3 inflammasome as well as its participation in diabetes and diabetic complications, providing novel insights into developing future therapeutic approaches for diabetes.

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The Construction and Analysis of lncRNA–miRNA–mRNA Competing Endogenous RNA Network of Schwann Cells in Diabetic Peripheral Neuropathy

Cited by 21 publications

References 68 publications

Comprehensive evaluation of differential long non‑coding RNA and gene expression in patients with cartilaginous endplate degeneration of cervical vertebra

Comprehensive evaluation of differential long non‑coding RNA and gene expression in patients with cartilaginous endplate degeneration of cervical vertebra

Using RNA-Seq to Explore the Hub Genes in the Trigeminal Root Entry Zone of Rats by Compression Injury

Emerging Role of LncRNA Regulation for NLRP3 Inflammasome in Diabetes Complications

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