The conserved surface M-protein SiMA of Streptococcus iniae is not effective as a cross-protective vaccine against differing capsular serotypes in farmed fish

Aviles, Fabian; Zhang, Meiman May; Chan, Janlin; Delamare-Deboutteville, J.; Green, Timothy J.; Dang, Cécile; Barnes, Andrew C.

doi:10.1016/j.vetmic.2012.08.018

Cited by 23 publications

(56 citation statements)

References 33 publications

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“…Vaccination against S. iniae is accomplished using formalin inactivated bacterins by intraperitoneal injection of fish under general anaesthetic in tilapia 12 , trout 13 , grouper 9 based on analysis of the first available genome sequences 21 , but these have not been uniformly effective [22][23][24] . With the falling cost of whole genome sequencing, using informatics to identify surface associated and secreted proteins that are conserved across different capsular serotypes has become a fast and cost-effective route to new experimental vaccines that may be cross-protective across multiple serotypes.…”

Section: Oleksandra Silayevamentioning

confidence: 99%

Vaccination against streptococcal infections in farmed fish

Barnes

Silayeva

2016

Microbiol. Aust.

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Aquaculture produces more than 50% of fish for human consumption and, in spite of major improvements since the adoption of injectable vaccines in the 1990s, bacterial diseases still account for considerable losses, particularly in tropical and warm temperate species. Streptococcosis, caused predominantly by Streptococcus iniae and S. agalactiae, manifests as a generalised septicaemia and meningitis followed by rapid mortality. Vaccination against streptococcal infections is difficult as a result of multiple, poorly defined serotypes and consequent vaccine escape (reinfection of previously vaccinated animals). However, genomics applied to reverse vaccinology is providing novel insights into diversity among these aquatic pathogens and is identifying cross-serotype targets that may be exploited for new generation streptococcal vaccines for aquaculture.Aquaculture reached a significant milestone in 2013 as global production for food use overtook beef production for the first time and now accounts for more than 50% of the global seafood supply 1 .Aquaculture has wrestled with social license throughout its rapid growth in developed economies, including Australia, through the

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Section: Oleksandra Silayevamentioning

confidence: 99%

Vaccination against streptococcal infections in farmed fish

Barnes

Silayeva

2016

Microbiol. Aust.

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“…A number of candidate S. iniae vaccines have been developed based on these factors [2]. However, subsequent reports demonstrate that vaccines, such as those based on the well studied M-protein, SiMA, are not effective in farmed fish [16]. Further research is therefore required to clarify the fundamental pathogenic mechanisms of S. iniae.…”

Section: Introductionmentioning

confidence: 99%

Comparative genome analysis of Streptococcus iniae DX09 reveals new insights into niche adaptation and competitive host colonisation ability

Liu¹,

Wang²,

Wang³

2017

Oncotarget

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Streptococcus iniae is a significant pathogen in a variety of marine and freshwater cultured fish species. Previous investigations on S. iniae have been limited to a single virulence gene or genome. However, different strains are associated with varying pathogenicity and niche adaptation properties. For comprehensive characterization of the genetic variations in S. iniae, whole-genome sequencing of S. iniae DX09 (isolated from diseased catfish) was performed and comparative genome analysis with eight S. iniae strains conducted to determine the virulence evolution patterns. Comparative analysis of all sequenced S. iniae revealed genome-genome variations, mainly in two plasticity zones, within genes encoding specific functions, such as the Ess/type VII secretion system and phosphoenolpyruvate-carbohydrate phosphotransferase system, reflecting adaptation to colonisation of specific host habitats. The plasticity zones analyzed in the S. iniae genome may be a paradigm rather than a unique combination of horizontal gene transfer and underlie the emergence of pathogenic Gram-positive bacteria.

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“…A formalin-killed bacterin vaccine based on strain QMA0285 isolated from wild Queensland grouper was prepared as previously described (Aviles et al, 2013). Cohorts of fish were anaesthetised with Aqui-S and vaccinated by i.p.…”

Section: Vaccine Preparation Fish Vaccination and Analysis Of Sera Fmentioning

confidence: 99%

“…The effect of pre-opsonisation of bacteria with antibodies on the phagocytosis and respiratory burst activities of HKL was determined in an assay previously described by (Aviles et al, 2013). Briefly, bacteria suspensions were incubated (55°C for 30 min) with HI non-immune serum (from PBS injected fish) or antiserum against QMA0285 vaccine (Ab) (10% v/v) for 1 hr at room temperature.…”

Section: Opsonophagocytosismentioning

confidence: 99%

On the origin of Group B Streptococcus from disease outbreaks in wild marine fish in Australia

Deboutteville¹

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Since 2007, 96 wild Queensland groupers, Epinephelus lanceolatus, were found dead from Karumba (Gulf of Carpentaria) in Northern Territory to Brisbane in southern Queensland, Australia. In twelve cases, Streptoccocus agalactiae (Group B Streptococcus, GBS) was isolated in pure culture from eyes and internal organs. GBS has been also isolated from other marine species (Javelin grunter, Giant catfish and Squaretail mullet) and caused an outbreak in three species of wild stingrays (Eastern shovelnose ray, Mangrove whipray and Estuary ray) in a public aquarium also in Queensland. These findings have raised public and industry concern over threats to human health from potential transmission between fish and humans, over environmental impacts on other aquatic animals, and over the potential threat posed to the growing Australian aquaculture industry in which, to date, there have been no reported cases of this disease in spite of the devastation caused to the industry overseas. Therefore, my thesis set out to determine whether GBS isolated from dead wild fish in Australia is indeed virulent in Queensland grouper and causative of mortality, to try to identify from where these infections in wild fish originated, to determine potential risk to human health and to other aquatic animals, and to begin to identify mechanisms by which these Australian isolates may cause fatal disease in Queensland grouper. This information may be employed in future risk assessment of potential human transfer, in mitigating further outbreaks in wild fish through understanding origin of infection, and in future development of preventative measures such as vaccination for farmed fish.In order to confirm that S. agalactiae was the cause of the mortality in wild fish, experimental challenges were conducted by injection, immersion and through the oral route. To better understand why these strains of GBS are pathogenic in fish, we conducted a series of cellular immune assays using juvenile Queensland grouper head-kidney leucocytes in the presence of different piscine and terrestrial isolates. Whole-genome sequencing analysis of the piscine and terrestrial isolates provided information on potential origin and potential for transfer between species, and also informed us on critical virulence factors that could be used during pathogenesis of GBS. Moreover, genome analysis gave intriguing insight into the evolution and adaption of GBS to fish as a host and to life in the aquatic environment. To determine putative origin of GBS in wild fish in Australia, multilocus sequence typing (MLST) and capsular polysaccharide (CPS) were derived from whole-genome sequences and revealed that all piscine isolates from Australia were serotype Ib and fell into ST-261 lineage first identified in 1986 from Tilapia in Israel. Overseas, ST-261 has only been found in fish and poikilotherm animals, and has never been associated with humans. High similarity and identical point mutations in the capsular operon of the piscine isolates suggests a common source of infection...

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The conserved surface M-protein SiMA of Streptococcus iniae is not effective as a cross-protective vaccine against differing capsular serotypes in farmed fish

Cited by 23 publications

References 33 publications

Vaccination against streptococcal infections in farmed fish

Vaccination against streptococcal infections in farmed fish

Comparative genome analysis of Streptococcus iniae DX09 reveals new insights into niche adaptation and competitive host colonisation ability

On the origin of Group B Streptococcus from disease outbreaks in wild marine fish in Australia

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