The current study examined if gender bias in the assessment process might be the reason why males are consistently found to exhibit more antisocial traits and behaviours than females. Employing the Leahy Antisocial Personality Scale (LAPS), a brief new self-report instrument for the assessment of antisocial traits, general population males (n = 216) were again found to exhibit more antisocial features than females (n = 391). Analysis of differential item functioning in the LAPS indicated that four items operated differently across the genders. To the extent that the LAPS measures DSM-IV-TR antisocial personality traits, fi ndings suggest that this criteria set is biased with regard to gender. Implications of this for the use of overall scores for broad diagnostic purposes may be minimal as the bias occurs in both directions; however, the fi ndings suggest that the adoption of differentially weighted criteria sets may be worthwhile in future studies examining gender differences in aspects of antisocial personality.