The conformation of adsorbed blood proteins by infrared bound fraction measurements

Morrissey, B. W.; Stromberg, Robert R.

doi:10.1016/0021-9797(74)90036-8

Cited by 177 publications

(48 citation statements)

References 26 publications

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“…Biopolymers (e.g., proteins) adsorb more strongly to hydrophobic than to hydrophilic surfaces (MacRitchie, 1972;van Oss, 1995;. Also, the adsorption of proteins, for example, onto hydrophobic surfaces is more denaturing and irreversible than adsorption onto hydrophilic surfaces (MacRitchie, 1972;Morrissey and Stromberg, 1974;van Oss, 1995). Finally, more hydrophobic microorganisms and particles are more avidly phagocytized by human phagocytic cells then Particles and fibers were used as received.…”

Section: Hydrophobicity Vs Hydrophilicitymentioning

confidence: 99%

Impact of Different Asbestos Species and Other Mineral Particles on Pulmonary Pathogenesis

Oss

Naim

Costanzo

et al. 1999

Clays and clay miner.

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Abstract--Factors that are potentially important in the pulmonary pathogenesis of asbestos and other mineral particles are: 1) morphology, 2) Fe-content, 3) solubility under intra-phagosomal conditions, 4) value and sign of the surface potential of the particle, 5) hydrophobicity or hydrophilicity, 6) capacity to activate phagocytic leukocytes, and 7) duration of exposure to the particles. The order of importance of these factors in causing severe or fatal pulmonary pathogenicity is estimated to be: 1 > 3 > 7 > 6 >> 5 > 4 > 2. The order of pathogenicity of the minerals is estimated as: amphibole asbestos: crocidolite, tremolite, amosite > erionite > serpentine asbestos: chrysotile > talc > silica > simple metal oxides. Particle length, duration of exposure to the particles, and pre-treatment of the particles may however enhance the pathogenic potential of any of the lower-ranked particles.

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Section: Hydrophobicity Vs Hydrophilicitymentioning

confidence: 99%

Impact of Different Asbestos Species and Other Mineral Particles on Pulmonary Pathogenesis

Oss

Naim

Costanzo

et al. 1999

Clays and clay miner.

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“…At this point the material is least soluble and so tends to accumulate at the clay/solution interface. Proteins, at least the globular type, apparently retain their gross native conformation on adsorption (Morrissey and Stromberg, 1974). The adsorption of enzymes by clays commonly leads to a reduction in their respective activity, and the pH-optimum for activity is shifted to more alkaline values (Ramirez-Martinez and McLaren, 1966).…”

Section: Formation and Properties Of Clay-polymer Complexesmentioning

confidence: 99%

Clay-Polymer Interactions: Summary and Perspectives

Theng¹

1982

Clays and clay miner.

391

193

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Abstract--The adsorption of uncharged polymers by clays is largely "entropy-driven." Polymer conformation changes from a random coil in solution to an extended form at the surface in which adsorbed polymer segments or trains alternate with loops and tails extending away from the surface. Although the net interaction energy, e, per segment-surface contact is small (--1 kT unit), the total energy of adsorption is large because the fraction of train segments, p, is commonly between 0.3 and 0.5. The adsorption isotherms are typically of the high-affinity type, and there is an apparent lack of desorption on dilution. Positively charged polymers (polycations) are adsorbed largely through electrostatic interactions between the cationic groups of the polymer and the negatively charged sites at the clay surface. Here e >> 1 kT unit and p > 0.7, leading to an almost complete collapse of the polymer chain onto the surface. Indeed, beyond a given level of adsorption charge reversal can occur in that the clay-polycation system effectively behaves as an anion exchanger. Little adsorption occurs with negatively charged polymers (polyanions) due to initial charge repulsion between the polymer and the clay surface. Acid pH, a high ionic strength, and the presence of polyvalent cations in the system enhance and promote polyanion adsorption. Uncharged polymers and polycations can enter the interlayer space of expanding 2:1 type layer silicates but polyanions generally fail to intercalate.The interactions of clays with biopolymers, such as proteins, nucleic acids, and polysaccharides, can be rationalized in similar terms. When intercalation occurs, the interlayer biopolymer is further stabilized against microbial (enzymatic) degradation giving rise to practical applications of clay-polymer complexes as flocculants and soil conditioners. Polyanions are effective as flocculants because of their large "grappling distance," whereas uncharged polymers are better suited as soil conditioners because they can spread over adjacent clay/soil particle surfaces like a coat of paint.

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“…55,56 This could explain why it was not possible to measure the hydrodynamic diameter of TiO 2 NPs suspended in medium containing Fib (Table 1), as the resulting interfacial agglomerates were probably responsible for the high polydispersity index of the Fib-TiO 2 suspension. Thus, the lower uptake of Fib-bound particles may be due to the interfacial agglomeration, which is relevant for in vivo studies as the interaction of NPs with the blood coagulant may reduce the bioavailable surface area of the particles and the concentration presented to the cells.…”

mentioning

confidence: 99%

The effect of blood protein adsorption on cellular uptake of anatase TiO2 nanoparticles

Allouni¹,

Gjerdet²,

Cimpan³

et al. 2015

IJN

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Protein adsorption onto nanoparticles (NPs) in biological fluids has emerged as an important factor when testing biological responses to NPs, as this may influence both uptake and subsequent toxicity. The aim of the present study was to quantify the adsorption of proteins onto TiO 2 NPs and to test the influence on cellular uptake. The surface composition of the particles was characterized by thermal analysis and by X-ray photoelectron spectroscopy. The adsorption of three blood proteins, ie, human serum albumin (HSA), γ-globulins (Glbs), and fibrinogen (Fib), onto three types of anatase NPs of different sizes was quantified for each protein. The concentration of the adsorbed protein was measured by ultraviolet-visible spectrophotometry using the Bradford method. The degree of cellular uptake was quantified by inductivity coupled plasma mass spectroscopy, and visualized by an ultra-high resolution imaging system. The proteins were adsorbed onto all of the anatase NPs. The quantity adsorbed increased with time and was higher for the smaller particles. Fib and Glbs showed the highest affinity to TiO 2 NPs, while the lowest was seen for HSA. The adsorption of proteins affected the surface charge and the hydrodynamic diameter of the NPs in cell culture medium. The degree of particle uptake was highest in protein-free medium and in the presence HSA, followed by culture medium supplemented with Glbs, and lowest in the presence of Fib. The results indicate that the uptake of anatase NPs by fibroblasts is influenced by the identity of the adsorbed protein.

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The conformation of adsorbed blood proteins by infrared bound fraction measurements

Cited by 177 publications

References 26 publications

Impact of Different Asbestos Species and Other Mineral Particles on Pulmonary Pathogenesis

Impact of Different Asbestos Species and Other Mineral Particles on Pulmonary Pathogenesis

Clay-Polymer Interactions: Summary and Perspectives

The effect of blood protein adsorption on cellular uptake of anatase TiO2 nanoparticles

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