The conditioned medium of murine and human adipose-derived stem cells exerts neuroprotective effects against experimental stroke model

Paracrine Neuroprotective Effects of Neural Stem Cells on Glutamate-Induced Cortical Neuronal Cell Excitotoxicity

“…31 Previously, neuroprotective effect of murine adipose derived stem cells conditioned medium on SH-SY5Y cell cultures survival has been shown after glutamate exposure. 32 Moreover, the neuroprotective effect of mesenchymal stem cells conditioned medium (MSCs-CM) has been shown on glutamate-induced excitotoxicity. This effect was related to cytokines and growth factors that MSCs secrete and generally modifies gene expression in neuronal cultures.…”

Transplantation of Adipose-Derived Stem Cells in Stroke

“…Therefore, based on the actions of these trophic factors, it is rational to attribute the observed enhancement of angiogenesis and neurogenesis as well as the abatement of apoptosis to the paracrine effects of the administered ADSC (Leu et al 2010;Gutierrez-Fernandez et al 2012). Consistently, another intriguing finding is the discovery of therapeutic effects against stroke using cell-free ADSC culture medium (Cho et al 2012;Egashira et al 2012). One study applying human adipose-derived stem cell-conditioned medium to the lateral ventricle of a rat model of ischemic stroke 8 h after MCAO continuously for 7 days demonstrated not only a reduction of infarction volume and preservation of motor function, but also enhanced endothelial cell proliferation, reduced neural cell apoptosis, and suppressed astrogliosis in the penumbra regions (Cho et al 2012).…”

Therapeutic Potential of Human Adipose-Derived Stem Cells in Neurological Disorders

“…administration before MCAO demonstrated significant reduction in infarct area and volume. In addition, rapid treatment of ASCs-CM immediately after MCAO was also effective, although administration 2 h after MCAO was not (41). I.v.…”

“…Pretreatment with ASCs-CM collected from the cultures of ASCs originally isolated from human subcutaneous adipose tissue or the fat pad of female C57BL/6-Tg mice significantly reduced glutamate-induced excitotoxicity in the SH-SY5Y or PC12 cells (41,43). Related to the glutamate-induced excitotoxicity, rat cortical neurons were also co-cultured with human ASCs-CM or ASCs that were separated from neurons with porous membrane to see the effects of ASCs-CM.…”