The Concept of Immunological Specificity

Silverstein, Arthur M.

doi:10.1016/b978-0-08-092583-7.50012-2

Cited by 2 publications

(1 citation statement)

References 33 publications

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“…Membrane protein complexes, or clusters of differentiation (CD), are one of a cell's primary means of communicating with the outside world. These proteins dot the surface of a cell and depending upon the function of a particular cell they can vary widely [12,13]. Much work has been done to fully characterize the variety of membrane protein expression levels between LNCaP cells and more advanced CaP cell lines [14,15].…”

Section: Introductionmentioning

confidence: 99%

Isolation of prostate cancer cell subpopulations of functional interest by use of an on-chip magnetic bead-based cell separator

Estes

Ouyang

et al. 2009

J. Micromech. Microeng.

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This work presents the design, fabrication and characterization of a modular magnetic bead-based cell separation device developed for the sequential sorting of a heterogeneous prostate cancer (CaP) cell population. The chief aim is cell sorting carried out on the basis of surface marker expression, serially selecting cellular subpopulations for capture by the use of antibody-coated magnetic beads. The markers of interest, prostate specific membrane antigen (PSMA) and CD10 were selected for their relevance to ongoing CaP development research. The separation device was fabricated out of plastic, by the use of cyclic olefin copolymer (COC) injection molding, nickel–iron electroplating and thermoplastic fusion bonding. Effective depletion and enrichment of cell subsets based on multiple surface markers was achieved. Various flow rates and incubation times were tested for optimizing the sorting procedure.

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Section: Introductionmentioning

confidence: 99%