The concentration of some inorganic ions and organic compounds in the luminal fluid of the human ductus deferens

Hinton, Barry T.; Pryor, J. P.; Hirsh, A. V.; Setchell, B. P.

doi:10.1111/j.1365-2605.1981.tb00730.x

Cited by 49 publications

(21 citation statements)

References 11 publications

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“…In the mouse, sperm are subjected to a change of about 100 mmol/kg (Yeung et al, 1999. Human sperm similarly are expelled from vas deferens fluid (342 mmol/kg: Hinton et al, 1981), to semen (342 mmol/kg: Yeung and Cooper, 2001), through cervical mucus (287 mmol/kg: Rossato et al, 1986), uterine fluid (284 mmol/kg: Casslein and Nielson, 1984), and into oviductal fluid containing follicular fluid (280 mmol/kg: Edwards, 1974). The anticipated rapid postejaculatory changes are in marked contrast to responses of somatic cells, where the need for RVD rarely arises (O'Neill, 1999).…”

Section: Importance Of Volume Regulationmentioning

confidence: 99%

“…However, no quantification supported these statements. One attempt to quantify the number of sperm with droplets within the rat cauda epididymidis estimated the percentage of sperm tail cross sections that would contain an image of a droplet were every (Hinton et al, 1981;Cooper and Yeung, unpubl. ), mouse , rat (Tuck et al, 1970;Levine and Marsh 1971;Jones 1978*;Eksittikul and Chulavatnatol, 1986;Clulow et al, 1994), hamster (Johnson and Howards, 1976;Jones, 1978*;D'Addario et al, 1980), rabbit (Jones 1974(Jones *, 1978, boar (Einarsson and Gustafsson, 1973;Jones, 1978*;Dacheux (thesis cited in Waites and Gladwell, 1982)), ram (Salisbury and Cragle, 1956*;Voglmayr et al, 1966;Jones, 1978*;White, 1973*), bull (Voglmayr et al, 1972;Gustafsson et al, 1974).…”

Section: Droplets On Mature Spermmentioning

confidence: 99%

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Acquisition of volume regulatory response of sperm upon maturation in the epididymis and the role of the cytoplasmic droplet

Cooper¹,

Yeung²

2003

Microscopy Res & Technique

169

134

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In certain cases of infertility in domestic species, and in the homozygous c-ros tyrosine kinase knockout mouse, males are infertile as a result of unopposed sperm swelling. This induces flagellar angulation, preventing their normal migration in the female tract. Angulation always occurs at the site of the cytoplasmic droplet located in mature sperm at the annulus (midpiece - principal piece junction). This article reviews the literature on the fate of the sperm's cytoplasmic droplet and suggests a role for it in volume regulation. A hypothesis is presented that during epididymal transit sperm acquire osmolytes accumulated by the epididymal epithelium. This is possibly driven by a mechanism of regulatory volume increase invoked by the hypertonicity of epididymal luminal fluid. These osmolytes are subsequently used for regulatory volume decrease after ejaculation into the relatively hypotonic female tract. Study of the mechanisms of sperm volume regulation may highlight new contraceptive leads.

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Section: Importance Of Volume Regulationmentioning

confidence: 99%

Section: Droplets On Mature Spermmentioning

confidence: 99%

Acquisition of volume regulatory response of sperm upon maturation in the epididymis and the role of the cytoplasmic droplet

Cooper¹,

Yeung²

2003

Microscopy Res & Technique

169

134

View full text Add to dashboard Cite

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“…Extracellular osmolality for posttesticular spermatozoa starts at 280-320 mmol kg −1 in the rete testis of various mammalian species, and reaches levels as high as 480 mmol kg −1 in the corpus region of the hamster epididymis (see Cooper and Yeung et al [13]). In man, there is no report on the osmolalities of epididymal fluid along the length, whereas that in the vas deferens is about 340 mmol kg −1 [14]. Subsequently, on ejaculation, sperm are confronted with a drop in extracellular osmolality, close to that of serum, in fresh seminal fluid (averaged 294 mmol kg −1 in men [15]) and female tract fluids (287, 284 and 268-280 mmol kg −1 in cervical mucus [16], uterine fluid [17] and oviductal fluid [18], respectively).…”

Section: Physiological Osmotic Challenges To Spermatozoa and Cell Volmentioning

confidence: 99%

Aquaporins in spermatozoa and testicular germ cells: identification and potential role

Yeung¹

2010

Asian J Androl

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Mammalian spermatozoa have relatively high water permeability and swell readily, as in the hypo-osmotic swelling test used in the andrology clinic. Physiologically, spermatozoa experience changes in the osmolality of the surrounding fluids in both the male and the female tracts on their journey from the testis to the ovum. Sperm volume regulation in response to such osmotic challenges is important to maintain a stable cell size for the normal shape and function of the sperm tail. Alongside ion channels for the fluxes of osmolytes, water channels would be crucial for sperm volume regulation. In contrast to the deep knowledge and numerous studies on somatic cell aquaporins (AQPs), the understanding of sperm AQPs is limited. Among the 13 AQPs, convincing evidence for their presence in spermatozoa has been confined to AQP7, AQP8 and AQP11. Overall, current findings indicate a major role of AQP8 in water influx and efflux for sperm volume regulation, which is required for natural fertilization. The preliminary data suggestive of a role for AQP7 in sperm glycerol metabolism needs further substantiation. The association of AQP11 with the residual cytoplasm of elongated spermatids and the distal tail of spermatozoa supports the hypothesis of more than just a role in conferring water permeability and also in the turnover and recycling of surplus cellular components made redundant during spermiogenesis and spermiation. This would be crucial for the maintenance of a germinal epithelium functioning efficiently in the production of spermatozoa.

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“…electrolytes and various organic compounds) [13,29]. The vas deferens epithelial cells are also known to accumulate various organic solutes, e.g.…”

Section: Introductionmentioning

confidence: 99%

Volume-sensitive chloride currents in primary cultures of human fetal vas deferens epithelial cells

et al. 1996

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Using the patch-clamp technique, we have identified a large, outwardly rectifying, Cl--selective whole-cell current in primary cultures of human vas deferens epithelial cells. Whole-cell currents were time- and voltage-dependent and displayed inactivation following depolarising pulses >/= 60 mV. Currents were equally permeable to bromide (PBr/PCl = 1.05 +/- 0.04), iodide (PI/PCl = 1. 06 +/- 0.07) and Cl-, but significantly less permeable to gluconate (PGluc /PCl = 0.23 +/- 0.03). Currents spontaneously increased with time after establishing a whole-cell recording, but could be inhibited by exposure to a hypertonic bath solution which reduced inward currents by 68 +/- 4%. Subsequent exposure of the cells to a hypotonic bath solution led to a 418 +/- 110% increase in inward current, indicating that these currents are regulated by osmolarity. 4,4'-Diisothiocyanatostilbene-2,2'-disulphonic acid (100 microM) produced a rapid and reversible voltage-dependent block (60 +/- 5% and 10 +/- 7% inhibition of current, measured at +/- 60 mV, respectively). Dideoxyforskolin (50 microM) also reduced the volume-sensitive Cl- current, but with a much slower time course, by 41 +/- 13% and 32 +/- 16% (measured at +/- 60 mV, respectively). Tamoxifen (10 microM) had no effect on the whole-cell Cl- current. These results suggest that vas deferens epithelial cells possess a volume-sensitive Cl- conductance which has biophysical and pharmacological properties broadly similar to volume-sensitive Cl- currents previously described in a variety of cell types.

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The concentration of some inorganic ions and organic compounds in the luminal fluid of the human ductus deferens

Cited by 49 publications

References 11 publications

Acquisition of volume regulatory response of sperm upon maturation in the epididymis and the role of the cytoplasmic droplet

Acquisition of volume regulatory response of sperm upon maturation in the epididymis and the role of the cytoplasmic droplet

Aquaporins in spermatozoa and testicular germ cells: identification and potential role

Volume-sensitive chloride currents in primary cultures of human fetal vas deferens epithelial cells

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