The composition of the nuclear antigens Sm and RNP of human rheumatic and connective tissue diseases and the relevance of their autoantibodies as probes for RNA processing mechanisms

MacGillivray, A.J.; Carroll, Anthony R.; Dahi, S.; Naxakis, G.; Sadaie, M. Reza; Wallis, C.M.; Tianyu, Jing

doi:10.1016/0014-5793(82)80033-1

Cited by 9 publications

References 34 publications

(13 reference statements)

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Characterization by human autoantibody of a nuclear antigen related to the cell cycle.

Barque¹,

Danon²,

Peraudeau³

et al. 1983

The EMBO Journal

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Using a serum from a patient with an autoimmune disease, we have recently described a novel 55 000‐dalton antigen (p55) in the nucleus of several animal cells including human ones. This antigen, designated PSL, was not related to the previously defined antigens recognized by sera from patients with systemic rheumatic diseases (Sm, n‐RNP, SS‐B, Scl‐70). We have now found that p55 is associated with chromatin structures as it is released from the nucleus of mink cell fibroblasts by saline + DNase treatments. Analysis by sucrose gradient centrifugation of the nuclear material released in these conditions indicated that p55 co‐migrated with core histones. Meanwhile, p55 was absent from the residual nuclear matrices (achromatinic nuclei). Localization of p55 in synchronized cells was performed by indirect immunofluorescence and immunoprecipitation. P55 appeared to accumulate in the nucleus during the S phase. Finally, it was not recognized by an anti‐SV40 tumor serum that specifically precipitated the protein p53, which has been recently related to cell proliferation. Thus, PSL an p53, although apparently not antigenically related, appear to be implicated in the same step of the cell cycle.

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Characterization by human autoantibody of a nuclear antigen related to the cell cycle.

Barque¹,

Danon²,

Peraudeau³

et al. 1983

The EMBO Journal

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Purification of snRNPs U1, U2, U4, U5 and U6 with 2,2,7-trimethylguanosine-specific antibody and definition of their constituent proteins reacting with anti-Sm and anti-(U1)RNP antisera.

Bringmann¹,

Rinke²,

Appel³

et al. 1983

The EMBO Journal

141

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Small nuclear ribonucleoprotein particles (snRNPs) of the U‐snRNP class from Ehrlich ascites tumor cells were purified in a one‐step procedure by affinity chromatography with antibodies specific for 2,2,7‐trimethylguanosine (m23.2.7G), which is part of the 5′‐terminal cap structure of snRNAs U1‐U5. Antibody‐bound snRNPs are desorbed from the affinity column by elution with excess nucleoside m23.2.7G; this guarantees maintenance of their native structure. The snRNPs U1, U2, U4, U5 and U6 can be recovered quantitatively from nuclear extracts by this procedure. Co‐isolation of U6 snRNP must be due to interactions between this and other snRNPs, as anti‐m23.2.7G antibodies do not react with deproteinized U6 snRNA. We have so far defined nine proteins of approximate mol. wts. 10 000, 12 000, 13 000, 16 000, 21 000, 28 000, 32 000, 34 000 and 75 000. Purified snRNPs react with anti‐(U1)RNP and with anti‐Sm antisera from patients with mixed connective tissue disease and from MRL/l mice. As determined by the protein blotting technique, six of the snRNP polypeptides, characterized by apparent mol. wts. 13 000, 16 000, 21 000, 28 000, 34 000 and 75 000, bear antigenic determinants for one or the other of the above autoantibody classes. This suggests strongly that the U‐snRNPs produced by the procedure described here are indeed representative of the snRNPs in the cell. With highly purified snRNPs available, investigation of possible enzymic functions of the particles may now be undertaken.

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The Sm antigens

Hoch

1996

Manual of Biological Markers of Disease

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The composition of the nuclear antigens Sm and RNP of human rheumatic and connective tissue diseases and the relevance of their autoantibodies as probes for RNA processing mechanisms

Cited by 9 publications

References 34 publications

Characterization by human autoantibody of a nuclear antigen related to the cell cycle.

Characterization by human autoantibody of a nuclear antigen related to the cell cycle.

Purification of snRNPs U1, U2, U4, U5 and U6 with 2,2,7-trimethylguanosine-specific antibody and definition of their constituent proteins reacting with anti-Sm and anti-(U1)RNP antisera.

The Sm antigens

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