The composition of the gut microbiota following early-life antibiotic exposure affects host health and longevity in later life

Lynn, Miriam A.; Eden, Georgina; Ryan, Feargal J.; Bensalem, Julien; Wang, Xuemin; Blake, Stephen J.; Choo, Jocelyn M.; Tee, Yee C.; Sribnaia, Anastasia; James, Jane; Benson, Saoirse C.; Sandeman, Lauren; Xie, Jianling; Hassiotis, Sofia; Sun, Emily; Martin, Alyce M.; Keller, Marianne; Keating, Damien J.; Sargeant, Timothy J.; Proud, Christopher G.; Wesselingh, Steve; Rogers, Geraint B.; Lynn, David J.

doi:10.1016/j.celrep.2021.109564

Cited by 33 publications

(36 citation statements)

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“… Perform further statistical analysis using R version 3.6.3, graphing using ggplot2 v3.3.0 and normalize all count data for library size prior to visualization or statistical testing. Note: As an example of the statistical analysis, please refer to the original article ( Lynn et al., 2021 ) and ( Lynn et al., 2018 ). The 16S rRNA gene sequence data has been deposited in the NCBI Sequence Read Archive under BioProject accession number PRJNA645716.…”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

“…Note: As an example of the statistical analysis, please refer to the original article ( Lynn et al., 2021 ) and ( Lynn et al., 2018 ). The 16S rRNA gene sequence data has been deposited in the NCBI Sequence Read Archive under BioProject accession number PRJNA645716.…”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

“…Accumulating evidence suggests that increased diversity of the gut microbiota is associated with healthy aging in humans ( Kong et al., 2016 ; Biagi et al., 2016 ). In our recent study ( Lynn et al., 2021 ), we investigated the impact of early life antibiotic exposure on otherwise healthy, normal chow fed, wildtype mice, monitoring these mice for >700 days in comparison to untreated control mice. We found that differences in the composition of the gut microbiota following antibiotic exposure differentially affected host health and longevity in later life.…”

Section: Before You Beginmentioning

confidence: 99%

“…Finally, we discuss how to address potential confounders such as the stochastic recolonization of the gut microbiota following antibiotic exposure. For complete details on the use and execution of this protocol, please refer to Lynn et al. (2021) .…”

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confidence: 99%

“…For complete details on the use and execution of this protocol, please refer to Lynn et al. (2021) .…”

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Protocol to assess the impact of early-life antibiotic exposure on murine longevity

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Section: Step-by-step Methods Detailsmentioning

confidence: 99%

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

Section: Before You Beginmentioning

confidence: 99%

mentioning

confidence: 99%

“…For complete details on the use and execution of this protocol, please refer to Lynn et al. (2021) .…”

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confidence: 99%

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Protocol to assess the impact of early-life antibiotic exposure on murine longevity

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Postnatal 14D is the Key Window for Mice Intestinal Development‐ An Insight from Age‐Dependent Antibiotic‐Mediated Gut Microbial Dysbiosis Study

2023

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The postnatal period is one of the critical windows for the structure-function development of the gastrointestinal tract and associated mucosal immunity. Along with other constituent members, recent studies suggest the contribution of gut microbiota in maintaining host health, immunity, and development. Although the gut microbiota's role in maintaining barrier integrity is known, its function in early life development still needs to be better understood. To understand the details of gut microbiota's effects on intestinal integrity, epithelium development, and immune profile, the route of antibiotic-mediated perturbation is taken. Mice on days 7(P7D), 14(P14D), 21(P21D) and 28(P28D) are sacrificed and 16S rRNA metagenomic analysis is performed. The barrier integrity, tight junction proteins (TJPs) expression, intestinal epithelial cell (IEC) markers, and inflammatory cytokines are analyzed. Results reveal a postnatal age-related impact of gut microbiota perturbation, with a gradual increase in the relative abundance of Proteobacteria and a reduction in Bacteroidetes and Firmicutes. Significant barrier integrity disruption, reduced TJPs and IECs marker expression, and increased systemic inflammation at P14D of AVNM-treated mice are found. Moreover, the microbiota transplantation shows recolonization of Verrucomicrobia, proving a causal role in barrier functions. The investigation reveals P14D as a critical period for neonatal intestinal development, regulated by specific microbiota composition.

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The Gut-Brain Axis

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The composition of the gut microbiota following early-life antibiotic exposure affects host health and longevity in later life

Cited by 33 publications

References 93 publications

Protocol to assess the impact of early-life antibiotic exposure on murine longevity

Protocol to assess the impact of early-life antibiotic exposure on murine longevity

Postnatal 14D is the Key Window for Mice Intestinal Development‐ An Insight from Age‐Dependent Antibiotic‐Mediated Gut Microbial Dysbiosis Study

Germ-Free Animals

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