The composition of bioactive compounds and antioxidant activity of Saskatoon berry (Amelanchier alnifolia Nutt.) genotypes grown in central Poland

Determination of phytochemical composition and antioxidant capacity of 22 old apple cultivars grown in Poland

“…Direct phloroglucinolysis of freeze-dried samples was performed as described by Lachowicz et al [18]. Fruit lyophilisates were weighed in an amount of 5 mg into 2-mL Eppendorf vials.…”

The effect of different maturity stages on phytochemical composition and antioxidant capacity of cranberry cultivars

“…Next, the slurry was centrifuged at 19,000g for 10 min, and the supernatant was filtered through a Hydrophilic PTFE 0.20 μm membrane (Millex Samplicity Filter, Merck, Darmstadt, Germany) and used for analysis. The content of polyphenols in individual extracts was determined by means of the ultra-performance liquid chromatography-photodiode array detector-mass spectrometry method [16]. All extractions were carried out in triplicate.…”

“…Qualitative (LC-Q-TOF-MS) and quantitative (UPLC-PDA-FL) analyses of polyphenols (anthocyanins, flavan-3-ols, flavonols, and phenolic acids) were performed as described previously by Lachowicz et al [16]. Separations of individual polyphenols were carried out using a UPLC BEH C18 column (1.7 μm, 2.1 × 100 mm, Waters Corporation, Milford, MA) at 30 °C.…”

“…The samples for analysis were prepared as described previously by Lachowicz et al [16]. Freeze-dried fruits (0.5 g) were mixed with 10 mL of MeOH/water (80:20%, v/v) + 1% HCl, sonicated at 20 °C for 15 min, and left for 24 h at 4 °C.…”

“…'Pilgrim', 'Stevens', and 'Ben Lear' was 2.3, 1.9, and 2.4 times higher than the same cultivars in this study in the commercially mature phase. It was suggested that the concentration of anthocyanins in cranberry fruits depends mainly on cultivar, growing place, environmental, climatic, and genetic factors [16]. The composition of anthocyanins increased remarkably in fruit cvs.…”