The composition of antibiotic resistance genes is not affected by grazing but is determined by microorganisms in grassland soils

Zheng, Zeyu; Li, Linfeng; Makhalanyane, Thulani P.; Xu, Chunming; Li, Kaihui; Xue, Kai; Xu, Cong; Qian, Ruyan; Zhang, Biao; Du, Jianqing; Yu, Hua; Cui, Xiaoyong; Wang, Yanfen; Hao, Yanbin

doi:10.1016/j.scitotenv.2020.143205

Cited by 25 publications

(16 citation statements)

References 67 publications

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“…Therefore, nutrients would exclusively provide carbon and energy sources for microbes carrying ARGs, leading to elevated normalized abundance of soil ARGs. This suggestion is also supported by regional observations across the Yangtze River Delta (28), Chinese forest (37), and North China grassland (38), where the contents of organic carbon, nitrogen, phosphorus, and potassium markedly controlled either soil ARG abundance, richness, or diversity. These results suggest that soil ARGs and their carriers at the global scale are subjected to environmental filtering, an essential deterministic process that shapes the composition, abundance, diversity, and function of microbial communities (13,23).…”

Section: Discussionsupporting

confidence: 66%

Global biogeography and projection of soil antibiotic resistance genes

et al. 2022

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Although edaphic antibiotic resistance genes (ARGs) pose serious threats to human well-being, their spatially explicit patterns and responses to environmental constraints at the global scale are not well understood. This knowledge gap is hindering the global action plan on antibiotic resistance launched by the World Health Organization. Here, a global analysis of 1088 soil metagenomic samples detected 558 ARGs in soils, where ARG abundance in agricultural habitats was higher than that in nonagricultural habitats. Soil ARGs were mostly carried by clinical pathogens and gut microbes that mediated the control of climatic and anthropogenic factors to ARGs. We generated a global map of soil ARG abundance, where the identified microbial hosts, agricultural activities, and anthropogenic factors explained ARG hot spots in India, East Asia, Western Europe, and the United States. Our results highlight health threats from soil clinical pathogens carrying ARGs and determine regions prioritized to control soil antibiotic resistance worldwide.

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Section: Discussionsupporting

confidence: 66%

Global biogeography and projection of soil antibiotic resistance genes

et al. 2022

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“…Grazers could propagate ARGs to clinical pathogens ( Du et al, 2020 ) and soils. In the Eurasian steppe, grazing reduced or did not alter ARGs ( Du et al, 2020 ; Zheng et al, 2021 ). In this work, the grazing decreased the resistance to Beta-Lactam (ampG) and Multidrug (emrA and acrA) subtypes, whereas increased the relative abundance of one ARGs type (bacitracin), Bacitracin (bceS), and Multidrug (lmrP) subtypes ( Supplementary Figures 4D, E ).…”

Section: Discussionmentioning

confidence: 98%

Grazing lowers soil multifunctionality but boosts soil microbial network complexity and stability in a subtropical grassland of China

Ding

Tian

et al. 2023

Front. Microbiol.

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IntroductionLong-term grazing profoundly affects grassland ecosystems, whereas how the soil microbiome and multiple soil ecosystem functions alter in response to two-decades of grazing, especially how soil microbiome (diversity, composition, network complexity, and stability) forms soil multifunctionality is rarely addressed.MethodsWe used a long-term buffalo grazing grassland to measure the responses of soil physicochemical attributes, stoichiometry, enzyme activities, soil microbial niche width, structure, functions, and networks to grazing in a subtropical grassland of Guizhou Plateau, China.ResultsThe evidence from this work suggested that grazing elevated the soil hardness, available calcium content, and available magnesium content by 6.5, 1.9, and 1.9 times (p = 0.00015–0.0160) and acid phosphatase activity, bulk density, pH by 59, 8, and 0.5 unit (p = 0.0014–0.0370), but decreased the soil water content, available phosphorus content, and multifunctionality by 47, 73, and 9–21% (p = 0.0250–0.0460), respectively. Grazing intensified the soil microbial carbon limitation (+78%, p = 0.0260) as indicated by the increased investment in the soil β-glucosidase activity (+90%, p = 0.0120). Grazing enhanced the complexity and stability of the bacterial and fungal networks but reduced the bacterial Simpson diversity (p < 0.05). The bacterial diversity, network complexity, and stability had positive effects, while bacterial and fungal compositions had negative effects on multifunctionality.DiscussionsThis work is an original attempt to show that grazing lowered multifunctionality via the reduced bacterial diversity and shifted soil bacterial and fungal compositions rather than the enhanced bacterial and fungal network complexities and stability by grazing. Protecting the bacterial diversity from decreasing, optimizing the composition of bacteria and fungi, and enhancing the complexity and stability of bacterial network may be conducive to improving the soil multifunction of grazing grassland, on a subtropical grassland.

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“…37 Mobile genetic elements (MGEs) can be transmitted through HGT within the same species or between different species. 38 Zheng et al 39 found that bacterial diversity and abundance were significantly positively correlated with MGEs ( P ≤ 0.01). The microbial diversity in the water samples after O 3 -TP disinfection was lower than that in raw and UV-TP disinfection water samples, which was not conducive to the transmission of MGEs among host bacteria.…”

Section: Resultsmentioning

confidence: 98%

“…All three phyla have been reported as possible host bacteria for ARGs. 39 Proteobacteria are the major ARBs in drinking water. 44 After O 3 -TP disinfection, the microbial community structure in the water samples became simple, and Proteobacteria reached 95.65%, which was greatly increased compared with that in raw water (86.03%).…”

Section: Resultsmentioning

confidence: 99%