The composition and variation of the BCR CDR3s in gastric cancer

Abstract

Gastric cancer (GC) is the fourth most common type of cancer and the second most common cause of cancer-associated mortality worldwide. B cell-associated autoantibodies against tumor-associated antigens are attractive biomarkers for the development of noninvasive serological tests for the early detection of cancer. This is due to their specificity and stability in the sera. In the present study multiplex polymerase chain reaction and Illumina high-throughput sequencing (HTS) was used to study the composition and variation of the B cell receptor (BCR) complimentary-determining region 3 (CDR3) in GC. The peripheral blood, cancer tissues and peri-cancer tissues were included from 7 patients with GC. On average there was a total of 403,959 CDR3 sequences, with 72,367 unique CDR3 nt sequences and 61,709 unique CDR3 aa sequences per sample identified, which are critical for further understanding the BCR repertoire in GC. The details of GC CDR3s may accelerate the screening process for possible new autoantigens and may provide additional information necessary for generating effective B cell targeted diagnosis and therapeutic strategies.

Cancer systems immunology

“…Elevated expression of a variety of normal proteins, expression of embryonic proteins and antigens, and expression of mutated proteins (neoantigens) all represent targets on tumor cells that can be recognized by BCRs and TCRs to elicit antitumor responses by the immune system. Consequently, cancer systems immunologists have employed targeted amplicon sequencing (typically, of cDNA derived from amplified TCR or BCR mRNA) to evaluate the BCR and TCR repertoires, providing insight into how lymphocytes respond to tumors ( Han et al, 2016 ; Page et al, 2016 ; Woodsworth et al, 2013 ; Sims et al, 2016 ; Linnemann et al, 2013 ; Jiang et al, 2019 ; Liu et al, 2018 ; Chaudhary and Wesemann, 2018 ; Zhang et al, 2017b ). Furthermore, researchers have used WES, frequently in combination with RNA-seq, to identify the range of potential neoantigens expressed by tumor cells as a consequence of their high mutation rates ( Garcia-Garijo et al, 2019 ).…”

Characterization of B-cell Receptor Heavy Chain Complementarity-determining Region 3 Repertoire Based on the Differences of Symbiotic Microorganisms in the Gut of Mice

“…Complementarity-determining region 3 (CDR3) is the most hyper-variable region in B cell receptor (BCR) gene, and the most critical structure in antigen recognition [12,13]. Comparing the diversity of BCR CDR3 repertoire, length distribution, IGHV, IGHD and IGHJ gene family expression and IGHV-IGHJ family distribution, the physiological and pathological state of the immune system can be evaluated [14,15]. Germ free (GF), gnotobiotic (GN), speci c pathogen free (SPF) and clean (CL) mice are conventional experimental model animals for studying the establishment and breaking of balance between intestinal micro ora and intestinal immune system.…”