The Composition and Structure of Plant Primary Cell Walls

O’Neill, Malcolm A.; York, William S.

doi:10.1002/9781119312994.apr0067

Cited by 68 publications

(64 citation statements)

References 198 publications

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“…In grasses, pectins and AGPs are the main Gal-containing cell wall polysaccharides (Carpita, 1996;Ishii, 1997;O'Neill & York, 2003). Higher Gal in foliar biomass may thus suggest more abundant pectin and/or AGPs in leaves than in stems.…”

Section: Miscanthus Cell Wall Glycansmentioning

confidence: 99%

A cell wall reference profile for Miscanthus bioenergy crops highlights compositional and structural variations associated with development and organ origin

et al. 2016

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Summary Miscanthus spp. are promising lignocellulosic energy crops, but cell wall recalcitrance to deconstruction still hinders their widespread use as bioenergy and biomaterial feedstocks. Identification of cell wall characteristics desirable for biorefining applications is crucial for lignocellulosic biomass improvement. However, the task of scoring biomass quality is often complicated by the lack of a reference for a given feedstock.A multidimensional cell wall analysis was performed to generate a reference profile for leaf and stem biomass from several miscanthus genotypes harvested at three developmentally distinct time points. A comprehensive suite of 155 monoclonal antibodies was used to monitor changes in distribution, structure and extractability of noncellulosic cell wall matrix glycans.Glycan microarrays complemented with immunohistochemistry elucidated the nature of compositional variation, and in situ distribution of carbohydrate epitopes. Key observations demonstrated that there are crucial differences in miscanthus cell wall glycomes, which may impact biomass amenability to deconstruction.For the first time, variations in miscanthus cell wall glycan components were comprehensively characterized across different harvests, organs and genotypes, to generate a representative reference profile for miscanthus cell wall biomass. Ultimately, this portrait of the miscanthus cell wall will help to steer breeding and genetic engineering strategies for the development of superior energy crops.

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Section: Miscanthus Cell Wall Glycansmentioning

confidence: 99%

A cell wall reference profile for Miscanthus bioenergy crops highlights compositional and structural variations associated with development and organ origin

et al. 2016

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“…The main types of side chains include ␣-1,5-linked l-arabinan with some 2-and 3-linked arabinose or arabinan branching, ␤-1,4-linked d-galactans with some 3-linked l-arabinose or arabinan branching and ␤-1,3-linked d-galactan with ␤-6-linked galactan or arabinogalactan branching (205). RG-I side branches may also contain ␣-l-Fucp, ␤-d-GlcpA, and 4-O-Me ␤-d-GlcpA residues (206). The composition and length of RG-I side chains varies between cell types and in different plant species (158,160).…”

Section: Lara Lrhamentioning

confidence: 99%

Cell Wall Polysaccharide Synthesis

Mohnen

Bar‐Peled

Somerville

2008

Biomass Recalcitrance

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“…Pectins characterized by high galacturonic acid content, were classified into four main groups: homogalacturonan (HGA), rhamnogalacturonan I (RGI), rhamnogalacturonan II (RGII), and xylogalacturonan (XGA) (Ridley et al 2001; Mohnen 2008). HGA polysaccharide, the major compounds of pectin, consists of galacturonosyl residues which may be methylesterified, acetylated and/or substituted with other subunits, including xylose (Schols et al 1995; O’Neill and York 2003). Chemical modifications in HGA chains, especially the process of de-esterification (and subsequent calcium binding), which is under the control of pectin methylesterase (PME, EC.…”

Section: Introductionmentioning

confidence: 99%

Low temperature caused modifications in the arrangement of cell wall pectins due to changes of osmotic potential of cells of maize leaves (Zea mays L.)

et al. 2016

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The cell wall emerged as one of the important structures in plant stress responses. To investigate the effect of cold on the cell wall properties, the content and localization of pectins and pectin methylesterase (PME) activity, were studied in two maize inbred lines characterized by different sensitivity to cold. Low temperature (14/12 °C) caused a reduction of pectin content and PME activity in leaves of chilling-sensitive maize line, especially after prolonged treatment (28 h and 7 days). Furthermore, immunocytohistological studies, using JIM5 and JIM7 antibodies, revealed a decrease of labeling of both low- and high-methylesterified pectins in this maize line. The osmotic potential, quantified by means of incipient plasmolysis was lower in several types of cells of chilling-sensitive maize line which was correlated with the accumulation of sucrose. These studies present new finding on the effect of cold stress on the cell wall properties in conjunction with changes in the osmotic potential of maize leaf cells.

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The Composition and Structure of Plant Primary Cell Walls

Cited by 68 publications

References 198 publications

A cell wall reference profile for Miscanthus bioenergy crops highlights compositional and structural variations associated with development and organ origin

A cell wall reference profile for Miscanthus bioenergy crops highlights compositional and structural variations associated with development and organ origin

Cell Wall Polysaccharide Synthesis

Low temperature caused modifications in the arrangement of cell wall pectins due to changes of osmotic potential of cells of maize leaves (Zea mays L.)

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