The Component Combined Amino Acids of Some Marine Phytoplankton Species

Abstract

Text-figs. 1 and 2)Gas-liquid and thin-layer chromatography have been used for the determination of 25 amino acids in the hydrolysates of 25 species of marine phytoplankton which had been grown in Erd-Schreiber medium. The general pattern of their distribution agrees with that found by earlier workers; the principal amino acids being glutamic acid, alanine, leucine and aspartic acids. Small amounts of 2-amino-M-butyric and 2-amino-wo-butyric acid together with compounds which have been tentatively identified as 4-amino-«-butyric acid and the 2-amino-and 2,5-diamino derivations of adipic acid were detected in many of the organisms. No differences were found which could be related to the taxonomy of the organisms.

Changes in Some Chemical Components of <i>Alexandrium catenella</i> and <i>Scrippsiella trochoidea</i> during Their Growth Cycles

“…Many published data show that glutamic acid is the most pre dominant amino acid component in dinoflagellates. 13,[25][26] These data agreed with the results of this study. Therefore, most of the organic nitrogen could be accounted for in terms of amino acid nitrogen.…”

Interspecific and intraspecific composition and variation of free amino acids in marine phytoplankton

“…Is it reflected by the extracellular pool measured in the culture medium, or in seawater? Previous results (Parsons et al 1961, Chau et al 1967, Daumas 1976, Enright et al 1986) have shown that amino acid composition was similar, regardless of the species and size of the phytoplankton. These results were obtained after hydrolysis of the samples, and were related to total amino acids (combined plus free), reflecting the composition of protein (combined fraction) rather than the free amino acids pool alone.…”

“…It appears that the intracellular pools of combined amino acids (Parsons et al 1961, Chau et al 1967, Daumas 1976, Enright et al 1986) and free amino acids Table 2. Total concentration of free amino acids and phytoplankton biomass, measured in the culture media of the 11 species.…”

“…during 2 phases of growth. Flux of amino acids has been estimated for each species between the 2 phases of growth (time-lag between phases: 4 to 13 d; see Moal et al [ Chau et al (1967) found no differences between algae in relation to their combined amino acids compositions. Similar conclusions were reached earlier by Parsons et al (1961).…”

Dissolved free amino acids in the marine environment: a carbon to nitrogen ratio shift during diatom blooms

“…Diatoms are characterized by a relative abundance of serine compared to other phytoplankton species (Chau et al 1967, Chuecas & Riley 1969. Therefore another possible origin of low ratio amino acids might be the synthesis of the diatom cell-wall.…”