The Complicated Effects of Extracellular Vesicles and Their Cargos on Embryo Implantation

Jiang, Nanxing; Li, Xuelian

doi:10.3389/fendo.2021.681266

Cited by 23 publications

(15 citation statements)

References 126 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Exosomes play an important role in intercellular communication and have been isolated from a wide variety of biofluids and tissues, including plasma, liver, uterine, semen, embryo, etc. [ 17 ]. The role of exosomes in intercellular communication comprises mediating the exchange of substances between cells, thereby changing the biological properties of the recipient cells [ 26 , 37 , 38 , 39 ].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

BOEC–Exo Addition Promotes In Vitro Maturation of Bovine Oocyte and Enhances the Developmental Competence of Early Embryos

Wei

Idrees

Sidrat

et al. 2022

Animals

View full text Add to dashboard Cite

Exosomes are nano-sized vesicles with abundant nucleic acids, proteins, lipids, and other regulatory molecules. The aim of this study was to examine the effect of BOEC-Exo on bovine in vitro oocyte maturation and in vitro embryo development. We found that a 3% Exo supplementation to IVM media significantly enhanced the oocyte maturation and reduced the accumulation of ROS in MII-stage bovine oocytes. Oocyte maturation related genes (GDF9 and CPEB1) also confirmed that 3% Exo treatment to oocytes significantly (p < 0.05) enhanced the oocyte maturation. Next, we cultured bovine cumulus cells and assessed the effects of 3% Exo, which showed a reduced level of apoptotic proteins (caspase-3 and p-NF-κB protein expressions). Furthermore, we examined the gap junction (CX43 and CX37) and cumulus cells expansion related genes (HAS2, PTX3, and GREM1) in cumulus–oocyte complexes (COCs), and all those genes showed significantly (p < 0.05) higher expressions in 3% Exo-treated COCs as compared with the control group. Moreover, peroxisome proliferator-activated receptors (PPARs) and lipid metabolism-related genes (CPT1 and FABP3) were also analyzed in both the control and 3% Exo groups and the results showed significant (p < 0.05) enhancement in the lipid metabolism. Finally, the oocytes matured in the presence of 3% Exo showed a significantly higher rate of embryo development and better implantation potential. Finally, we concluded that Exo positively influenced bovine oocyte in vitro maturation and improved the early embryo’s developmental competence.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Exosomes can act not only as biomarkers, but also as targeted drug-delivery systems, and thus hold promise for an advanced comprehension of reproductive diseases [ 17 ]. However, the precise mechanisms of action of exosomes in oocyte maturation have not been elucidated yet.…”

Section: Introductionmentioning

confidence: 99%

BOEC–Exo Addition Promotes In Vitro Maturation of Bovine Oocyte and Enhances the Developmental Competence of Early Embryos

Wei

Idrees

Sidrat

et al. 2022

Animals

View full text Add to dashboard Cite

show abstract

“…Exosomes are involved in the suitable interaction between the embryo and uterine endometrium required for successful embryo implantation during pregnancy [ 92 ]. Endometrial epithelium can release exosomes into the uterine cavity and transfer specific miRNAs to the blastocyst or endometrial epithelial cells to enhance embryo implantation [ 93 ]. Comparing the miRNAs of the exosomes and their producer cells demonstrated that 13 of the 227 miRNAs were specific for exosomes and promoted embryo implantation [ 94 ].…”

Section: The Roles Of Exosomes In Embryo Implantationmentioning

confidence: 99%

The implications of exosomes in pregnancy: emerging as new diagnostic markers and therapeutics targets

et al. 2022

View full text Add to dashboard Cite

Extracellular vehicles (EVs) are a heterogeneous group of cell and membranous particles originating from different cell compartments. EVs participate in many essential physiological functions and mediate fetal-maternal communications. Exosomes are the smallest unit of EVs, which are delivered to the extracellular space. Exosomes can be released by the umbilical cord, placenta, amniotic fluid, and amniotic membranes and are involved in angiogenesis, endothelial cell migration, and embryo implantation. Also, various diseases such as gestational hypertension, gestational diabetes mellitus (GDM), preterm birth, and fetal growth restriction can be related to the content of placental exosomes during pregnancy. Due to exosomes' ability to transport signaling molecules and their effect on sperm function, they can also play a role in male and female infertility. In the new insight, exosomal miRNA can diagnose and treat infertilities disorders. In this review, we focused on the functions of exosomes during pregnancy.

show abstract

“…Oviduct is the place where occur oocyte transportation, fertilization, and initial embryo development. Oviductal extracellular vesicles (oEVs), as the main components of oviduct fluid, can orchestrate gamete/embryo-oviduct and embryomaternal interactions, hence supporting oocyte mature (in canine), embryonic development, and motility, capacitation, and fertilizing ability of sperm [14,15]. The contents of oEVs (mRNAs, miRNAs, and proteins) are dynamic at different stages of the estrous cycle, suggesting that cargos of oEVs are under hormonal control [16].…”

Section: Evs Derived From the Oviductmentioning

confidence: 99%

Roles of Extracellular Vesicles in Human Reproduction

Zhou¹,

Zhang²,

Lian³

et al. 2022

Extracellular Vesicles - Role in Diseases, Pathogenesis and Therapy

View full text Add to dashboard Cite

Extracellular vesicles (EVs) are newly identified as cell-to-cell communication mediators that carry and transfer various regulatory molecules. Recent studies have shown that EVs play important roles in normal physiology and pathological conditions of human reproduction. In the female reproductive system, EVs in follicular fluid, oviduct fluid, and uterine luminal fluid are considered as vehicles to regulate follicular development, oocyte maturation and mediate embryo–maternal crosstalk to affect embryo implantation and pregnancy. In the male reproductive system, prostasomes and epididymosomes are involved in regulating sperm maturation, motility, capacitation, acrosome reaction, and fertilization. EVs transmitted cargos also play important roles in reproduction-related pathologies, such as polycystic ovarian syndrome, endometriosis, pregnancy complications, male infertility, and gynecological malignant tumors. In view of the important roles in the reproductive system, EVs may be used as biomarkers or therapeutic targets for reproductive abnormalities and related diseases. In this chapter, we sorted EVs in human reproduction through their physical/pathological functions and mechanisms, and listed several EVs as biomarkers and clinical therapeutic applications in the future.

show abstract

The Complicated Effects of Extracellular Vesicles and Their Cargos on Embryo Implantation

Cited by 23 publications

References 126 publications

BOEC–Exo Addition Promotes In Vitro Maturation of Bovine Oocyte and Enhances the Developmental Competence of Early Embryos

BOEC–Exo Addition Promotes In Vitro Maturation of Bovine Oocyte and Enhances the Developmental Competence of Early Embryos

The implications of exosomes in pregnancy: emerging as new diagnostic markers and therapeutics targets

Roles of Extracellular Vesicles in Human Reproduction

Contact Info

Product

Resources

About