The complexity of microRNAs in human cancer

Kwan, Jennifer; Psarianos, Pamela; Bruce, Jeff; Yip, Kenneth W.; Liu, Fei‐Fei

doi:10.1093/jrr/rrw009

Cited by 56 publications

(39 citation statements)

References 39 publications

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“…MicroRNAs (miRNAs) are small ncRNAs with a length of 22 nucleotides, and their expression is closely related to cancer progression. 13 MiRNAs-targeted therapy technology has developed new strategies for cancer treatment. 14 With the deepening of the research on circRNAs mechanism, it has been found that circRNAs can act as complementary RNAs (ceRNAs) to participate in the regulation of target genes of miRNAs.…”

Section: Introductionmentioning

confidence: 99%

<p>Circ_0058124 Upregulates MAPK1 Expression to Promote Proliferation, Metastasis and Metabolic Abilities in Thyroid Cancer Through Sponging miR-940</p>

Sun¹,

Chen²,

Lv³

et al. 2020

OTT

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Background: Thyroid cancer (TC) is an endocrine disease, and its progression is regulated by many factors, including circular RNAs (circRNAs). However, as a new circRNA, the role of circ_0058124 in TC is worth further exploration. Methods: The expression levels of circ_0058124, microRNA-940 (miR-940) and mitogen-activated protein kinase 1 (MAPK1) were assessed by quantitative polymerase chain reaction (q-PCR). The circular characteristic of circ_0058124 was identified by oligo (dT) 18 primers, Ribonuclease R (RNase R) and Actinomycin D (ActD), and its localization was determined by nuclear-cytoplasmic separation assay. Also, cell proliferation was detected by colony formation assay, and cell migration and invasion were assessed by transwell assay. Further, Seahorse XF Extracellular Flux Analyzer was used to measure the oxygen consumption rate (OCR) of cells. Besides, dual-luciferase reporter, RNA immunoprecipitation (RIP) and RNA pull-down assays were used to identify the mechanism of circ_0058124. Western blot (WB) analysis was used to test the MAPK1 protein level. In addition, mice xenograft models were constructed to test the effect of circ_0058124 on TC tumor growth in vivo. Results: Circ_0058124 was highly expressed in TC and is a stable cyclic transcript, mainly located in the cytoplasm. Circ_0058124 knockdown suppressed proliferation, migration, invasion and metabolic abilities in TC cells. MiR-940 could be absorbed by circ_0058124, and the inhibition effect of its overexpression on TC progression could be reversed by overexpressed-circ_0058124. MAPK1 was a target of miR-940, and the suppression effect of its silencing on TC progression could be inverted by miR-940 inhibitor. Besides, MAPK1 expression was regulated by circ_0058124 and miR-940. Interference of circ_0058124 also reduced TC tumor growth in vivo. Conclusion: Circ_0058124 might play a carcinogenic role in TC progression by regulating the miR-940/MAPK1 axis, which might provide a new idea for the treatment of TC.

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Section: Introductionmentioning

confidence: 99%

<p>Circ_0058124 Upregulates MAPK1 Expression to Promote Proliferation, Metastasis and Metabolic Abilities in Thyroid Cancer Through Sponging miR-940</p>

Sun¹,

Chen²,

Lv³

et al. 2020

OTT

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“…MicroRNAs (miRNAs), a group of noncoding, small RNA molecules, negatively regulate gene expression via directly interacting with the 3′‐UTR of target gene . MiRNAs regulate many biological roles including cell differentiation, apoptosis, proliferation, cycle, invasion, migration, metastasis, and cell death . Increasing studies demonstrated that miRNA was aberrantly expressed in several tumors such as gastric cancer, bladder cancer, breast cancer, pancreatic cancer, and laryngeal squamous cell carcinoma .…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‐379 inhibits laryngeal carcinoma cell proliferation and invasion through directly targeting TCF‐4

Wei

Zhang

Zhao

et al. 2019

The Kaohsiung J of Med Scie

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The expression pattern, functions, and detailed regulatory mechanisms of miR‐379 in laryngeal carcinoma remain unknown. In the present study, we aimed to uncover novel potential miR‐379 targets and shed light on its roles in laryngeal carcinoma. The expression level of miR‐379 was measured based on the data obtained from the TCGA database and the cell lines. After miR‐379 mimic transfection, cell proliferation, invasion and migration assay, and wound‐healing assay in HEp‐2 cell line were implemented to evaluate the effects of miR‐379 on laryngeal carcinoma in vitro. The target genes for miR‐379 in silico were predicted and validated using luciferase reporter assay. The miR‐379 expression level was reduced in laryngeal carcinoma tissues and cell lines. Moreover, miR‐379 overexpression inhibited the cell proliferation, migration, and invasion of laryngeal carcinoma cells. TCF‐4 was detected as a direct target of miR‐379 in laryngeal carcinoma. Furthermore, restored TCF‐4 expression rescued the inhibitory roles of miR‐379 overexpression on cell proliferation, migration, and invasion.Our study for the first time demonstrated that miR‐379/TCF‐4 might involve in the progression of laryngeal carcinoma, and miR‐379 appears to serve as a novel tumor suppressor in laryngeal carcinoma.

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“… 2 A number of single miRNA biomarkers are associated with the clinical outcome of NSCLC, 3 , 4 and recent reports suggest that various signatures comprising multiple miRNA biomarkers may be more powerful in classifying cancer subtypes than single miRNAs. 5 Nevertheless, the diagnostic efficiency of these miRNA signatures could not be replicated in additional separate patient cohorts. 6 …”

Section: Introductionmentioning

confidence: 99%

Establishment and validation of a 7-microRNA prognostic signature for non-small cell lung cancer

Wang

Guo

Dong

et al. 2018

CMAR

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PurposeA series of microRNAs (miRNAs) have been identified as associated with the survival of patients with non-small-cell lung cancer (NSCLC). The aim of the present study was to explore whether combination of these experimentally validated individual miRNA biomarkers could be used to further increase their prognostic power in NSCLC.Patients and methodsBased on previously validated NSCLC prognostic miRNAs, gene signatures that could discriminate high-risk subgroups with poor clinical outcome in four NSCLC miRNA expression datasets (GSE13937, GSE16025, The Cancer Genome Atlas Lung Adenocarcinoma, and TCGA lung squamous cell carcinoma) were developed using the SurvMicro tool. The potential of the miRNA signature established was validated by quantitative real-time PCR analysis of clinical NSCLC samples, and its prognostic power evaluated using the survivalROC method.ResultsWe developed two miRNA signatures with prognostic significance for NSCLC, comprising 12- and 7-miRNAs. The 7-miRNA signature (miR-148b, miR-365, miR-32, miR-375, miR-21, miR-125b, and miR-155) was a subset of a 12-miRNA set that retained prognostic power across NSCLC cohorts. Compared with previously established miRNA signatures, our 7-miRNA signature has similar potential, while comprising fewer miRNA components. The prognostic ability of the 7-miRNA signature was validated experimentally in an independent NSCLC cohort using real-time PCR (HR=3.4847, 95% CI=1.3693–8.8680, P=0.0092), and this signature, combined with tumor pathological stage, had superior prognostic ability compared with tumor stage alone.ConclusionOur data indicate that the established 7-miRNA signature is simple, robust, and may have greater clinical prognostic utility for patients with NSCLC.

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The complexity of microRNAs in human cancer

Cited by 56 publications

References 39 publications

<p>Circ_0058124 Upregulates MAPK1 Expression to Promote Proliferation, Metastasis and Metabolic Abilities in Thyroid Cancer Through Sponging miR-940</p>

<p>Circ_0058124 Upregulates MAPK1 Expression to Promote Proliferation, Metastasis and Metabolic Abilities in Thyroid Cancer Through Sponging miR-940</p>

MicroRNA‐379 inhibits laryngeal carcinoma cell proliferation and invasion through directly targeting TCF‐4

Establishment and validation of a 7-microRNA prognostic signature for non-small cell lung cancer

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