The Complex Tale of the <i>achaete–scute</i> Complex: A Paradigmatic Case in the Analysis of Gene Organization and Function During Development

Garcı́a-Bellido, Antonio; Celis, José F. de

doi:10.1534/genetics.109.104083

Cited by 77 publications

(77 citation statements)

References 79 publications

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“…Similar to their counterparts in the central brain and VNC, these NBs express the bHLH transcription factors Dpn and Ase, as well as Miranda (Mira) (Wallace et al, 2000;Egger et al, 2007). Dpn is a member of the Hairy-E(spl) (HES)-family of bHLH repressors (Bier et al, 1992), while Ase belongs to the Achaete-Scute complex family of proneural bHLH activators (Garcia-Bellido & de Celis, 2009). Mira is a coiled-coil protein that serves as an adaptor for basally localized components of the asymmetric cell division machinery (Ikeshima-Kataoka et al, 1997).…”

Section: Sequential Generation Of Medulla Neurons By Neuroblastsmentioning

confidence: 99%

A Challenge of Numbers and Diversity: Neurogenesis in theDrosophilaOptic Lobe

Apitz¹,

Salecker²

2014

Journal of Neurogenetics

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The brain areas that endow insects with the ability to see consist of remarkably complex neural circuits. Reiterated arrays of many diverse neuron subtypes are assembled into modular yet coherent functional retinotopic maps. Tremendous progress in developing genetic tools and cellular markers over the past years advanced our understanding of the mechanisms that control the stepwise production and differentiation of neurons in the visual system of Drosophila melanogaster. The postembryonic optic lobe utilizes at least two modes of neurogenesis that are distinct from other parts of the fly central nervous system. In the first optic ganglion, the lamina, neuroepithelial cells give rise to precursor cells, whose proliferation and differentiation depend on anterograde signals from photoreceptor axons. In the second optic ganglion, the medulla, the coordinated activity of four signaling pathways orchestrates the gradual conversion of neuroepithelial cells into neuroblasts, while a specific cascade of temporal identity transcription factors controls subtype diversification of their progeny.

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Section: Sequential Generation Of Medulla Neurons By Neuroblastsmentioning

confidence: 99%

A Challenge of Numbers and Diversity: Neurogenesis in theDrosophilaOptic Lobe

Apitz¹,

Salecker²

2014

Journal of Neurogenetics

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“…In Drosophila neurogenesis, Da acts as a heterodimer with one of five proneural group A proteins. Of these, Achaete (Ac), Scute (Sc), and Lethal of scute (L'sc) are paralogous proteins encoded from a gene cluster, the ac-sc complex (ASC) (24). The other two are Atonal and Amos (30,34).…”

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confidence: 99%

Essential Roles of Da Transactivation Domains in Neurogenesis and in E(spl)-Mediated Repression

Zarifi

Kiparaki

Koumbanakis

et al. 2012

Molecular and Cellular Biology

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E proteins are a special class of basic helix-loop-helix (bHLH) proteins that heterodimerize with many bHLH activators to regulate developmental decisions, such as myogenesis and neurogenesis. Daughterless (Da) is the sole E protein in Drosophila and is ubiquitously expressed. We have characterized two transcription activation domains (TADs) in Da, called activation domain 1 (AD1) and loop-helix (LH), and have evaluated their roles in promoting peripheral neurogenesis. In this context, Da heterodimerizes with proneural proteins, such as Scute (Sc), which is dynamically expressed and also contributes a TAD. We found that either one of the Da TADs in the Da/Sc complex is sufficient to promote neurogenesis, whereas the Sc TAD is incapable of doing so. Besides its transcriptional activation role, the Da AD1 domain serves as an interaction platform for E(spl) proteins, bHLH-Orange family repressors which antagonize Da/Sc function. We show that the E(spl) Orange domain is needed for this interaction and strongly contributes to the antiproneural activity of E(spl) proteins. We present a mechanistic model on the interplay of these bHLH factors in the context of neural fate assignment.

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“…Sensory organ precursors (SOPs) of macrochaetes emerge in groups of cells called proneural clusters (PNCs) at the third instar larval stage (Huang et al, 1991). Within PNC, the basic helix-loop-helix (bHLH) transcription factors of Achaete (Ac) and Scute (Sc) encoded by the achaete-scute complex (AS-C) (Ghysen and DamblyChaudiere, 1988;Garcia-Bellido and de Celis, 2009) are expressed and gradually accumulate in one of these cells (Cubas et al, 1991;Romani et al, 1989). Finally, the cell with the highest Ac-Sc expression is chosen to become a SOP by lateral inhibition mediated by Notch (N) signaling pathway (Simpson, 1990;Simpson et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

DREF is Critical for Drosophila Bristle Development by Regulating Endoreplication in Shaft Cells

Kawamori

Yamaguchi

2011

Cell Struct. Funct.

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ABSTRACT. DREF (DNA replication-related element-binding factor) plays important roles in replication and proliferation in vivo by regulating transcription of various genes. However, due to a lack of appropriate cell biological studies in vivo, roles of DREF during a single cell development are poorly understood. To address this question, we focused our attention on macrochaetes bristle development system. Utilizing cell lineage analysis focusing on a single posterior scutellar (PSC) macrochaete sensory organ precursor (SOP) lineages in combination with GAL4/UAS targeted expression system for DREF double strand RNA, we revealed that DREF plays no apparent role in differentiation process during SOP formation. Rather, DREF regulates the timing of asymmetric cell division but perhaps plays no direct role in differentiation during asymmetric cell division. Most importantly, DREF affected replication and growth in shaft cells and/or socket cells. Further analysis revealed that DREF is necessary but not sufficient for nuclear growth and protein synthesis in shaft cells. Finally, it could be demonstrated that DREF plays a critical role in regulating pcna transcription in endocycling shaft cells. All these results provide evidence that DREF plays critical roles, especially in endoreplication process of bristle development, at least in part by regulating the pcna gene expression.

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The Complex Tale of the achaete–scute Complex: A Paradigmatic Case in the Analysis of Gene Organization and Function During Development

Cited by 77 publications

References 79 publications

A Challenge of Numbers and Diversity: Neurogenesis in theDrosophilaOptic Lobe

A Challenge of Numbers and Diversity: Neurogenesis in theDrosophilaOptic Lobe

Essential Roles of Da Transactivation Domains in Neurogenesis and in E(spl)-Mediated Repression

DREF is Critical for Drosophila Bristle Development by Regulating Endoreplication in Shaft Cells

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