The complex structure of polyhydroxybutyrate (PHB) granules: four orthologous and paralogous phasins occur in Ralstonia eutropha

Pötter, Markus; Müller, Helena; Reinecke, Frank; Wieczorek, Roman; Fricke, Florian; Bowien, Botho; Friedrich, Bärbel; Steinbüchel, Alexander

doi:10.1099/00221287-150-9-3089

Cited by 22 publications

(31 citation statements)

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“…Additionally, a galactose oxidase with a positively charged coil has been attached to polyhydroxybutyrate synthase (PhaC) protein particles with a negatively-charged helical coil [6]. However, the catalytic capabilities in both methods were limited because only the exposed PHA granule surface were available for the enzyme immobilization [7]. The charge interactions between the enzyme and the protein particles could also be hindered under high ionic conditions.…”

Section: Introductionmentioning

confidence: 98%

Generation of catalytic protein particles in Escherichia coli cells using the cellulose-binding domain from Cellulomonas fimi as a fusion partner

Choi

Lee

et al. 2011

Biotechnol Bioproc E

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The cellulose-binding domain (CBD) of a Cellulomonas fimi exo-glucanase was translationally fused with β-glucuronidase (GusA) from Escherichia coli and β-glycosidase (BglA) from Thermus caldophilus, respectively. Two fusion proteins (GusA-CBD and BglA-CBD) were expressed as insoluble aggregates in cells and isolated by centrifugation of the cell lysates. Interestingly, activity assays revealed that > 90% of the catalytic activity of both proteins was localized in the insoluble fractions. For example, the GusA-CBD particles exhibited 21 units per mg protein, which corresponded to 19% specific activity of the highly purified soluble GusA. The specific activity increased further up to 42 units per mg protein when treated with either sonication or chaotropic L-arginine. These results demonstrate that fusion with CBD family II may activate catalytic protein particles in E. coli cells, and that internal proteins of the particles are also active. Finally, the protein particles were tested in repeated batch operations after being cross-linked with chemicals, indicating that they have potential as a new preparation for immobilized biocatalysts.

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Section: Introductionmentioning

confidence: 98%

Generation of catalytic protein particles in Escherichia coli cells using the cellulose-binding domain from Cellulomonas fimi as a fusion partner

Choi

Lee

et al. 2011

Biotechnol Bioproc E

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“…Among the proteins associated to PHA granules, phasins constitute a very diverse group are the most dominant proteins of relatively small molecular size (124,140,141). A few that have been studied in detail revealed that they are associated with a structural role of PHA granules and may have several functions such as coating, stabilizing granules as structural proteins by non-covalently attached to the polyester core of granules ( Figure 5) (30,135,142,143,144) or activating genes and/or enzymes involved in PHA synthesis (124). Phasins promote PHA biosynthesis and their copy number has impact on PHA granule size (143,144).…”

Section: Structure Of Pha Granulesmentioning

confidence: 99%

“…A few that have been studied in detail revealed that they are associated with a structural role of PHA granules and may have several functions such as coating, stabilizing granules as structural proteins by non-covalently attached to the polyester core of granules ( Figure 5) (30,135,142,143,144) or activating genes and/or enzymes involved in PHA synthesis (124). Phasins promote PHA biosynthesis and their copy number has impact on PHA granule size (143,144). Phasins are inhibiting individual granules from coalescing and agglutinating with other granules and acting as a barrier between the polymer and other cellular components (123,124,145).…”

Section: Structure Of Pha Granulesmentioning

confidence: 99%

Bacterial polyhydroxyalkanoates-eco-friendly next generation plastic: Production, biocompatibility, biodegradation, physical properties and applications

Muhammadi

Shabina

Afzal

et al. 2015

Green Chemistry Letters and Reviews

266

139

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Polyhydroxyalkanoates (PHAs) are intracellular aliphatic polyesters synthesized as energy reserves, in the form of water-insoluble, nano-sized discrete and optically dense granules in cytoplasm by a diverse bacteria and some archae under conditions of limiting nutrients in the presence of excess carbon source. Bacteria synthesize different PHAs from coenzyme A thioesters of respective hydroxyalkanoic acid, and degrade intracellularly for reuse and extracellularly in natural environments by other microorganisms. In vivo, PHAs exist as amorphous mobile liquid and water-insoluble inclusions but in vitro, exhibit material and mechanical properties, ranging from stiff and brittle crystalline to elastomeric and molding, similar to petrochemical thermoplastics. Further, they are hydrophobic, isotactic, biocompatible and exhibit piezoelectric properties. But as commodity plastics their applications are limited by high production cost, low yield, in vivo degradation, complexity of technology and difficulty of extraction. Therefore, to replace the conventional plastic with PHAs, it is prerequisite to standardize the PHA production systems.

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“…Phasins (PhaP1-PhaP7 R.eu ; ~16-20 kDa) are the best example of non-catalytic surface proteins [26][27][28][29]. Among the reported phasins, PhaP1 is well studied and therefore well characterized.…”

Section: Divergent Roles Of Surface Proteinsmentioning

confidence: 99%

Recombinant surface engineering to enhance and expand the potential of biologically produced nanoparticles: A review

Kutralam-Muniasamy

Pérez-Guevara

2017

Process Biochemistry

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The complex structure of polyhydroxybutyrate (PHB) granules: four orthologous and paralogous phasins occur in Ralstonia eutropha

Cited by 22 publications

References 0 publications

Generation of catalytic protein particles in Escherichia coli cells using the cellulose-binding domain from Cellulomonas fimi as a fusion partner

Generation of catalytic protein particles in Escherichia coli cells using the cellulose-binding domain from Cellulomonas fimi as a fusion partner

Bacterial polyhydroxyalkanoates-eco-friendly next generation plastic: Production, biocompatibility, biodegradation, physical properties and applications

Recombinant surface engineering to enhance and expand the potential of biologically produced nanoparticles: A review

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