The complete sequence of the Cydia pomonella granulovirus genome

Abstract

The nucleotide sequence of the DNA genome of Cydia pomonella granulovirus (CpGV) was determined and analysed. The genome is composed of 123 500 bp and has a GMC content of 45n2 %. It contains 143 ORFs of 150 nucleotides or more that show minimal overlap. Onehundred-and-eighteen (82n5 %) of these putative genes are homologous to genes previously identified in other baculoviruses. Among them, 73 are homologous to genes of Autographa californica nucleopolyhedrovirus (AcMNPV), whereas 108 and 98 are homologous to genes of Xestia c-nigrum GV (XcGV) and Plutella xylostella GV (PxGV), respectively. These homologues show on average 37n4 % overall amino acid sequence identity to those from AcMNPV and 45 % to those from XcGV and PxGV. The CpGV gene content was compared to that of other baculoviruses. Several genes reported to have major roles in baculovirus biology were not found in the CpGV genome, such as gp64, the major budded virus glycoprotein gene in some nucleopolyhedroviruses, and lef-7, involved in DNA replication. However, the CpGV genome encodes the large and small subunits of ribonucleotide reductase, three inhibitor of apoptosis (iap) homologues and two protein tyrosine phosphatases. The CpGV, PxGV and XcGV genomes present a noticeably high level of conservation of gene order and orientation. A striking feature of the CpGV genome is the absence of typical homologous repeat sequences. However, it contains one major repeat region and 13 copies of a single 73-77 bp imperfect palindrome.

Identification and Characterization of a Conserved Baculoviral Structural Protein ODVP-6E/ODV-E56 from Choristoneura fumiferana Granulovirus

“…The size of baculoviral ODVP-6E/ODV-E56 proteins varies in ranges between 351 aa in PxGV to 379 aa in CfMNPV. The localization and orientation of the odvp-6e/odv-e56 gene on the ChfuGV genome is similar to that described for CpGV (Luque et al, 2001). The 5 noncoding region of odvp-6e/odve56 was analyzed with the aim of detecting all possible cisacting elements and possible transcription start sites (tss).…”

Genomic sequence analysis of a fast-killing isolate of Spodoptera frugiperda multiple nucleopolyhedrovirus

“…One of these conserved ORFs, sf130/ac29, was almost entirely contained within a larger (Nakai et al, 2003); ClbiNPV (GenBank accession no. DQ504428); Lymantria dispar MNPV (LdMNPV-Cl5-6; Kuzio et al, 1999); HzSNPV (Chen et al, 2002); HearNPV isolates C1 (Zhang et al, 2005) and G4 ; AcMNPV-C6 (Ayres et al, 1994); SpltNPV-G2 (Pang et al, 2001); and CpGV-M1 (Luque et al, 2001). See text for definitions.…”

“…To examine the relationship of SfMNPV-3AP2 with other group II NPVs, phylogenetic trees were inferred from two sets of aligned sequences: (i) the concatenated aligned sequences for 28 baculovirus genes from completely sequenced group II NPVs as well as AcMNPV-C6 (Ayres et al, 1994) and Cydia pomonella granulovirus (CpGV; Luque et al, 2001) (Fig. 2a), and (ii) the concatenated aligned partial amino acid sequences of polyhedrin and late expression factors 8 and 9 (polh, lef-8 and lef-9; Fig.…”

Hyphantria cunea Nucleopolyhedrovirus, a Novel Baculovirus Isolated from Fall Webworm in Korea

“…Baculoviruses have two types of anti-apoptotic genes, p35 and iap (Clem, 2001). Baculovirus iap genes are currently classified into five members, iap1-iap5, based on amino acid sequence homology (Maguire et al, 2000;Luque et al, 2001). The kind, number and anti-apoptotic function of iap genes are varied according to the baculovirus.…”