The complete sequence of a unique rna species synthesized by a DI particle of VSV

Schubert, Manfred; Keene, Jack D.; Lazzarini, Robert A.; Emerson, Suzanne U.

doi:10.1016/0092-8674(78)90086-7

Cited by 76 publications

(60 citation statements)

References 10 publications

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“…In the third model, if common initiation or termination points are used, then internal deletion(s) of a part of the progenitor viral RNA segment must occur in the formation of one of the DI RNAs. Current evidence for Sendai (23) and vesicular stomatitis virus (24,25) support the idea that DI RNAs share a common 5'-terminal sequence with the genomic RNA. The only exception is vesicular stomatitis virus DI LT, which lacks the 5'-end sequence (26).…”

Section: Discussionmentioning

confidence: 75%

“…1 left) (20,21), in the RNA of defective Semliki Forest virus (22), and in the.RNA of defective Sendai virus (23). For vesicular stomatitis particles and Sendai virus it appears that the 5' terminus of the genomic RNA is a common initiation sequence for DI RNAs (22)(23)(24)(25). A similar mechanism could be responsible for the generation of these influenza viral DI RNAs.…”

Section: Discussionmentioning

confidence: 99%

“…In the first model, such DI RNAs could be synthesized by using a number of distinct initiation and termination points. However, most of the DI viral RNAs isolated from different viruses (15,(22)(23)(24)(25) do not appear to be formed in this fashion. In the second model, DI RNA could have either a 5' or a 3' initiation point depending upon whether + or -strand RNA, respectively, was used as a template in the formation of DI RNA.…”

Section: Discussionmentioning

confidence: 99%

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Sequence relationships among defective interfering influenza viral RNAs.

Davis¹,

Nayak²

1979

Proc. Natl. Acad. Sci. U.S.A.

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Each clone of ts-52 and ts+ WSN influenza virus, when serially passaged at high multiplicity, gives rise to defective interfering (DI) virus with a unique set of new RNA species. The new RNAs (DI RNA) from several DI viruses were examined by the technique of RNase T1 oligonucleotide two-dimensional electrophoresis. It was found that each DI RNA arises from a specific segment of standard viral RNA. All DI RNA studied arose from the viral polymerase genes (P1, P2, and P3). DI RNAs originating from the same polymerase gene were interrelated. Certain of these DI RNAs appeared to contain completely overlapping nucleotide sequences. Others contained both overlapping and nonoverlapping nucleotide sequences. The latter DI RNAs may be formed from the progenitor viral RNA segment by a mechanism other than a common initiation (or termination) point and a simple deletion from one end.

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Section: Discussionmentioning

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Sequence relationships among defective interfering influenza viral RNAs.

Davis¹,

Nayak²

1979

Proc. Natl. Acad. Sci. U.S.A.

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“…

The function of these VSV-specific small RNAs is unknown.

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confidence: 99%

“…Among the vesicular stomatitis virus (VSV)-specific RNA species synthesized during infection, a small RNA, sedimenting at approximately 6 S in sucrose gradients, has been identified and related to the synthesis of defective interfering (DI) particles (2). More recently, small RNAs that are synthesized in vtro by purified VSV DI particles have been characterized and sequenced (3)(4)(5)(6)(7)(8).…”

mentioning

confidence: 99%

Synthesis of a small RNA in cells coinfected by standard and defective interfering particles of vesicular stomatitis virus.

Rao¹,

Huang²

1979

Proc. Natl. Acad. Sci. U.S.A.

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(3)(4)(5)(6)(7)(8). These small RNAs resemble the "leader" sequence described by Colonno and Banerjee (9-11) in that they contain 46-48 nucleotides with a (p)ppAp at the 5' end and they lack poly(A) at the 3' end.The function of these VSV-specific small RNAs is unknown. To study their function, conditions were sought for reproducibly detecting their synthesis in infected cells. Neither standard infectious VSV nor DI particles of VSV alone led to the synthesis of any small virus-specific RNA. Only when cells were coinfected with standard and DI particles was a small RNA detected. The synthesis of this small RNA correlated directly with the replication of the genome of DI particles. Both RNA species were absent from coinfected cells when cycloheximide was present or when DI particles were irradiated with UV light prior to infection. These data can be interpreted in terms of interference with the growth of standard VSV by DI particles. MATERIALS AND METHODSCells and Viruses. Suspension cultures of baby hamster kidney (BHK) cells, originally obtained from Amiya Banerjee, were grown in Joklik's modified minimal essential medium supplemented with 5% fetal calf serum. Cloned and sucrose gradient-purified standard infectious particles of VSV of the Indiana serotype (San Juan strain) and two different DI particles derived from the same San Juan standard VSV were used (12, 13). The DI-T particle has been characterized in detail (14-18). It is one-third the size of standard VSV and contains sequences from the 5' end of the genome. DI0.52 particles were selected from cloned VSV by multiple undiluted passages in mouse L cells and then subsequent growth in BHK cells. DI0.52 particles are twice the size of DI-T particles and also map at the 5' end of the VSV genome (unpublished observations). Both DI particles were sucrose gradient-purified and their multiplicities were determined as described (19).Labeling of Infected Cells. BHK cells were infected at a multiplicity of 20 with either standard VSV or DI particles alone or were coinfected with equal amounts of standard VSV or DI particles at a total multiplicity of 40. Detailed procedures for infection and labeling with 32P in the presence of actinomycin D have been published (1).Preparation and Analysis of RNA. RNAs from VSV particles or from infected cells were extracted as described (1). To analyze total cytoplasmic RNA, ethanol-precipitated RNA was resuspended in 5 ,gl of deionized H20 and mixed with an equal volume of double-strength electrophoresis buffer [6 M urea, the dyes xylene cyanol (XC) and bromphenol blue, and 0.01% sucrose]. To denature the large RNA species fully, they were first treated with 90% dimethyl sulfoxide and heated at 50'C for 30 min. This treatment was not necessary for small RNA. For the separation of virion RNA and mRNA (large RNAs), 1.5% agarose/6 M urea, pH 3.8 was used (1,20). Electrophoresis was at 100 V at 40C until the XC was 5 cm from the bottom of the gel. Then, the 1.5% agarose gel was dried and exposed to x-ray film as described (1).Fo...

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Defective Viral Particles

López

2021

Virology

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The complete sequence of a unique rna species synthesized by a DI particle of VSV

Cited by 76 publications

References 10 publications

Sequence relationships among defective interfering influenza viral RNAs.

Sequence relationships among defective interfering influenza viral RNAs.

Synthesis of a small RNA in cells coinfected by standard and defective interfering particles of vesicular stomatitis virus.

Defective Viral Particles

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