The complete plastid genome and nuclear genome markers provide molecularevidence for the hybrid origin of Pulsatilla × hackelii Pohl.

Szczecińska, Monika; Łazarski, Grzegorz; Bilska, Katarzyna; Sawicki, Jakub

doi:10.3906/bot-1610-28

Cited by 12 publications

(16 citation statements)

References 37 publications

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“…Also, the sectional position of a new or hybrid species can be defined using some nuclear or chloroplast DNA regions, and it can be very useful, especially for some specimens that cannot be distinguished morphologically (Dirmenci and Duman, 2017). Szczecińska et al (2017) studied the hybrid origin of Pulsatilla × hackelii Pohl. using whole chloroplast and nuclear markers.…”

Section: Discussionmentioning

confidence: 99%

A new species and a new natural hybrid of Origanum L. (Lamiaceae) from the west of Turkey

Dırmencı¹,

Yazıcı²,

Özcan³

et al. 2018

Turk J Bot

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The species of the genus Origanum L. are strongly aromatic perennial herbs and subshrubs. The Origanum species have many morphological and chemical varieties. Carvacrol, thymol, linalool, and p-cymene are some of their abundant chemical components. Origanum species are widely used as tea and herbs in folk medicine. They have antispasmodic, antimicrobial, expectorant, antioxidant, anticholinesterase, and carminative effects. (Başer and Kırımer, 2006;Yılmaz et al., 2017). Therefore, the use of Origanum plants is very common as tea, as additives to foods, and in traditional remedies for the treatment of infectious diseases and the prevention of cancer and gastrointestinal system diseases (Marrelli et al., 2010).The genus Origanum (Lamiaceae, subfamily: Nepetoideae, tribe: Mentheae, subtribe: Menthineae) comprises 43 species (51 taxa) and 15 hybrids worldwide (Ietswaart, 1980(Ietswaart, , 1982. The species are mainly concentrated in the temperate regions of the Mediterranean area. Twenty-three species (26 taxa), 8 hybrids, and one suspected species have been recorded in Turkey, which has the most species. Twenty of those are endemic (Ietswaart, 1982;Davis et al., 1988;Duman, 2000). The species are classified into eight sections: Sect. Amaracus (Gleditsch) Vogel ( 4species), Sect. Anatolicon Ietsw. (2 species), Sect. Breviflamnetum Ietsw. (7 species), Sect. Longitubus Ietsw. (1 species), Sect. Chilocalyx (Briq.) Ietsw. (3 species), Sect. Majorana (Mill.) Benth. (3 species), Sect. Origanum (1 species, 4 taxa), and Sect. Prolaticorolla Ietsw. (1 species).Origanum dictamnus L., indicated as a suspected species in the Flora of Turkey, belongs to the section Amaracus. To date, O. dictamnus has only been recorded from Crete, where it is considered endemic. An unflowered specimen from Aydın-Priene (Fors-Maj. 665) was collected and identified as O. dictamnus in the Flora of Turkey (Ietswaart, 1980(Ietswaart, , 1982. This specimen (from Aydın-Priene) was different from O. dictamnus in respect to the unbranched indumentum. However, although this specimen did not have flowers, it was reported that this specimen did not, in fact, belong to O. dictamnus (Ietswaart, 1980(Ietswaart, , 1982. Based on this note stated in the Flora of Turkey and the Eastern Aegean Islands, in order to collect new materials, a field trip was made to the environs of Priene, an ancient city on the Dilek Peninsula/Kuşadası (Figure 1). Despite covetable specimens not being collected from Priene, some

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Section: Discussionmentioning

confidence: 99%

A new species and a new natural hybrid of Origanum L. (Lamiaceae) from the west of Turkey

Dırmencı¹,

Yazıcı²,

Özcan³

et al. 2018

Turk J Bot

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“…Chloroplast genome structural rearrangements and inversions in Anemone and other related genera have been previously reported based on genetic mapping by restriction enzyme site methods (Hoot and Palmer, 1994 ; Johansson, 1999 ). In recent years, several complete chloroplast genomes of Ranunculaceae have been published (Chen et al, 2015 ; Park et al, 2015 , 2017 ; Li et al, 2016 ; Park and Park, 2016 ; Jiang et al, 2017b ; Lim et al, 2017 ; Szczecinska et al, 2017 ; Liu et al, 2018 ). Recently, Jiang et al ( 2017b ) and Liu et al ( 2018 ) published plastome sequences of Anemoclema and Clematis s.l .…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Complete Chloroplast Genomes of Anemoclema, Anemone, Pulsatilla, and Hepatica Revealing Structural Variations Among Genera in Tribe Anemoneae (Ranunculaceae)

Liu

Ding

et al. 2018

Front. Plant Sci.

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Structural rearrangements of Anemone species' chloroplast genome has been reported based on genetic mapping of restriction sites but has never been confirmed by genomic studies. We used a next-generation sequencing method to characterize the complete chloroplast genomes of five species in the tribe Anemoneae. Plastid genomes were assembled using de novo assembling methods combined with conventional Sanger sequencing to fill the gaps. The gene order of the chloroplast genomes of tribe Anemoneae was compared with that of other Ranunculaceae species. Multiple inversions and transpositions were detected in tribe Anemoneae. Anemoclema, Anemone, Hepatica, and Pulsatilla shared the same gene order, which contained three inversions in the large single copy region (LSC) compared to other Ranunculaceae genera. Archiclematis, Clematis, and Naravelia shared the same gene order containing two inversions and one transposition in LSC. A roughly 4.4 kb expansion region in inverted repeat (IR) regions was detected in tribe Anemoneae, suggesting that this expansion event may be a synapomorphy for this group. Plastome phylogenomic analyses using parsimony and a Bayesian method with implementation of partitioned models generated a well resolved phylogeny of Ranunculaceae. These results suggest that evaluation of chloroplast genomes may result in improved resolution of family phylogenies. Samples of Anemone, Hepatica, and Pulsatilla were tested to form paraphyletic grades within tribe Anemoneae. Anemoclema was a sister clade to Clematis. Structual variation of the plastid genome within tribe Anemoneae provided strong phylogenetic information for Ranunculaceae.

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“…The indumentum is a distinctive character for distinguishing N. viscida and N. nuda subsp. nuda. As with many hybrids (Clevinger and Panero 2000; Baumel et al 2002; Lowe and Abbott 2004; Liu et al 2017; Szczecińska et al 2017; Jaźwa et al 2018; Van Valkenburg et al 2018; Dirmenci et al 2019b), N. × tmolea hybrid individuals show transition/intermediate characteristics between N. viscida and N. nuda subsp. nuda. However, some specimens of N. × tmolea have higher similarities to N. nuda subsp. nuda, while others have high morphological similarities to N. viscada .…”

Section: Resultsmentioning

confidence: 99%

Defining phylogenetic relationship of Nepeta x tmolea and its parents via DNA barcoding

Özcan

2019

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Nepeta viscida and N. nuda subsp. nuda and N. × tmolea were examined in this study. Mainly fresh leaf pieces, dried with silica grains, were used for DNA extraction procedures via DNA isolation kits. Standard PCR techniques were executed using three different primer sets (one nuclear DNA region (nrITS) and two chloroplast DNA regions (rpl32-trnL and trnA(Leu)-trnA(Phe)-trnL-F). DNA sequences were analysed and evaluated using different molecular approaches and software. Consequently, the inconstant molecular structure and hybrid nature of N. × tmolea specimens were shown and interpreted in this study. According to our result, N. × tmolea have some intermediate characters compared to its parents. nrITS data give more information phylogenetically, and also the most polymorphic loci are seen in nrITS data. Morphological and molecular data contribute to define separation of N. × tmolea. Consequently, the inconstant molecular structure and hybrid nature of N. × tmolea specimens were shown and interpreted in this study.

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The complete plastid genome and nuclear genome markers provide molecularevidence for the hybrid origin of Pulsatilla × hackelii Pohl.

Cited by 12 publications

References 37 publications

A new species and a new natural hybrid of Origanum L. (Lamiaceae) from the west of Turkey

A new species and a new natural hybrid of Origanum L. (Lamiaceae) from the west of Turkey

Comparative Analysis of Complete Chloroplast Genomes of Anemoclema, Anemone, Pulsatilla, and Hepatica Revealing Structural Variations Among Genera in Tribe Anemoneae (Ranunculaceae)

Defining phylogenetic relationship of Nepeta x tmolea and its parents via DNA barcoding

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