The complete nucleotide sequence of the genome of Barley yellow dwarf virus-RMV reveals it to be a new Polerovirus distantly related to other yellow dwarf viruses

Abstract

The yellow dwarf viruses (YDVs) of the Luteoviridae family represent the most widespread group of cereal viruses worldwide. They include the Barley yellow dwarf viruses (BYDVs) of genus Luteovirus, the Cereal yellow dwarf viruses (CYDVs) and Wheat yellow dwarf virus (WYDV) of genus Polerovirus. All of these viruses are obligately aphid transmitted and phloem-limited. The first described YDVs (initially all called BYDV) were classified by their most efficient vector. One of these viruses, BYDV-RMV, is transmitted most efficiently by the corn leaf aphid, Rhopalosiphum maidis. Here we report the complete 5612 nucleotide sequence of the genomic RNA of a Montana isolate of BYDV-RMV (isolate RMV MTFE87, Genbank accession no. KC921392). The sequence revealed that BYDV-RMV is a polerovirus, but it is quite distantly related to the CYDVs or WYDV, which are very closely related to each other. Nor is BYDV-RMV closely related to any other particular polerovirus. Depending on the gene that is compared, different poleroviruses (none of them a YDV) share the most sequence similarity to BYDV-RMV. Because of its distant relationship to other YDVs, and because it commonly infects maize via its vector, R. maidis, we propose that BYDV-RMV be renamed Maize yellow dwarf virus-RMV (MYDV-RMV).

Characterising maize viruses associated with maize lethal necrosis symptoms in sub Saharan Africa

“…Of the sites studied, 69% also had the recently discovered Maize yellow mosaic virus (MYMV). nucleotide identity to an isolate from Mozambique (Acc: FJ882098) 26 and a virus with 81% nucleotide identity to the sequence of the recently reclassified Maize yellow dwarf virus 27 . Further examination using a more recent BLAST database revealed this virus to be MYMV 28 .…”

Mild and severe cereal yellow dwarf viruses differ in silencing suppressor efficiency of the P0 protein

“…ORF0 of CYDV-RPS (former CYDV-RPV-Mex1; NC 002198, (Krueger et al, 2013)) was synthesized and cloned into a pBluescript vector by GeneCust (http://www.genecust.com) before being PCR amplified using the primers indicated in Supplementary Table 1 and transferred into a pDONR TM /Zeo vector. Clones were controlled by sequencing and further recombined into the Gateway (Invitrogen) destination vector pGWB12 (Nakagawa et al, 2007) with an N-terminal FLAG tag for agro-infiltration tests to generate pBinP0 RPV and pBinP0 RPS (designed here by P0 RPV and P0 RPS ).…”

Dynamic maize responses to aphid feeding are revealed by a time series of transcriptomic and metabolomic assays

“…Additionally, heavy accumulation of aphid honeydew on maize tassels can block pollen shed and thereby reduce seed set (Foott and Timmins, 1973;Carena and Glogoza, 2004). Indirect damage comes from the fact that the corn leaf aphid is the most effective vector of several plant viruses, including Maize yellow dwarf virus and Barley yellow dwarf virus, which are among the most economically important diseases of cereal crops and forage grasses (El-Muadhidi et al, 2001;Hawkes and Jones, 2005;Power et al, 2011;Jarošová et al, 2013;Krueger et al, 2013). As protection against aphids and other insect pests, maize plants implement not only constitutive defenses but also inducible responses that require broad shifts in gene expression and biochemical pathways (Thompson and Goggin, 2006;Meihls et al, 2012).…”