The complete nucleotide sequence of RNAβ from the type strain of barley stripe mosaic virus

Gustafson, Gary; Armour, Susan

doi:10.1093/nar/14.9.3895

Cited by 68 publications

(57 citation statements)

References 23 publications

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“…3 and 4 c, d; Gustafson & Armour, 1986). Probably only a few nucleotides were missing in this clone for completion of the sequence encoding the PSLV tic product (Fig.…”

Section: Open Reading Framesmentioning

confidence: 95%

“…Alignments of nucleotide and amino acid sequences were generated by the program MULTALIN (Corpet, 1988), and slightly modified by hand. Comparisons were done with the nucleotide and amino acid sequences of the BSMV strain ND18 (Gustafson & Armour, 1986;Gustafson et al, 1987).…”

Section: Aminoacylation Of Viral Rnas and Analysis Of Internal Poly(a)mentioning

confidence: 99%

“…1 a). The BSMV CP (fla product) could not be seen among the L-[35S]methionine-labelled products as it contains no internal Met residues (Gustafson & Armour, 1986). Finally, the 20K protein which was produced upon in vitro translation of total virion RNA of PSLV also has its counterpart among the BSMV RNA translation products.…”

Section: Translation Aminoacylation and Rnase H Testsmentioning

confidence: 99%

“…Assuming that PSLV R N A ends in the sequence C C A 3' as for BSMV R N A (Kozlov et al, 1984), we added artificial poly(A) tails to it, and used the specific --237 (Gustafson & Armour, 1986;Gustafson et al, 1987). ORFs are shown by boxes drawn approximately to scale.…”

Section: Open Reading Framesmentioning

confidence: 99%

“…Gustafson & Armour, 1986;Gustafson et al, 1987Gustafson et al, , 1989. BSMV RNAs are capped and their 3' non-coding region encompasses an internal polydisperse poly(A) and the 3' tRNA-like structure accepting tyrosine (Agranovsky et al, 1979(Agranovsky et al, , 1981(Agranovsky et al, , 1982Kozlov et al, 1984).…”

Section: Introductionmentioning

confidence: 99%

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Poa semilatent virus, a hordeivirus having no internal polydisperse poly(A) in the 3' non-coding region of the RNA genome

Agranovsky

Karasev

Novikov

et al. 1992

Journal of General Virology

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RNA from the Hungarian isolate of poa semilatent virus (PSLV) directed in vitro synthesis of 120K, 75K, 25K (coat protein) and 20K polypeptides. In vitro translation of PSLV RNA was blocked by the cap analogue, m7Gpp, thus suggesting that the virus RNA was capped. PSLV RNA could be aminoacylated with [~4C]tyrosine in vitro. The sequence of 1-5 kb from the 3' end of the PSLV RNA y component revealed two open reading frames (ORFs) separated by a uridinerich intergenic region. The putative product of the incomplete 5'-proximal ORF showed a close amino acid sequence similarity with the C-terminal segment of the 7a protein (putative RNA replicase) encoded in the barley stripe mosaic virus (BSMV) RNA 7, and the 20K product of the 3'-proximal ORF was found to be related to the 17K 7b product of BSMV. The sequence of 0-8 kb from the 3' end ofPSLV RNA fl encompassed two (incomplete) overlapping ORFs whose putative products are related to the tic and fld proteins encoded in the similarly arranged ORFs of BSMV RNA ft. Nucleotide sequence homology between the respective parts of the two hordeivirus genomes was restricted to the ORF for 7a, the spacer between the ORFs for ya and yb, and the 3' non-coding region, particularly the 95 nucleotide segment at the 3' end representing a tRNAlike structure. Despite limited sequence conservation beyond this segment, the entire 3' non-coding region of PSLV RNA could be folded in a tight pseudoknotted structure closely resembling that of BSMV RNA. Surprisingly, the 'signature' sequence typical for BSMV RNA, internal polydisperse poly(A) intercalated between the coding part and the 3' tRNA-like structure, was not detected in the PSLV genome. Instead, the virus RNA contained several oligoadenylate stretches spaced by other residues, close to the junction of its coding and 3' non-coding portions.

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“…3 and 4 c, d; Gustafson & Armour, 1986). Probably only a few nucleotides were missing in this clone for completion of the sequence encoding the PSLV tic product (Fig.…”

Section: Open Reading Framesmentioning

confidence: 95%

Section: Aminoacylation Of Viral Rnas and Analysis Of Internal Poly(a)mentioning

confidence: 99%

Section: Translation Aminoacylation and Rnase H Testsmentioning

confidence: 99%

Section: Open Reading Framesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Poa semilatent virus, a hordeivirus having no internal polydisperse poly(A) in the 3' non-coding region of the RNA genome

Agranovsky

Karasev

Novikov

et al. 1992

Journal of General Virology

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BSMV genome mediated expression of a foreign gene in dicot and monocot plant cells.

Joshi

1990

The EMBO Journal

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Barley stripe mosaic virus (BSMV) possesses a tripartite genome composed of RNAs alpha, beta and gamma that have been cloned into in vitro transcription vectors from which infectious transcripts can be obtained. The BSMV genome has been engineered here to serve as an expression vector in plant protoplasts. Open reading frame (ORF) b of RNA beta, encoding a non‐structural protein, was replaced by the firefly luciferase (luc) reporter gene to yield RNA beta 2‐luc. In the presence of both RNAs alpha and gamma, RNA beta 2‐luc mediated efficient expression of the luc gene upon transfection into tobacco and maize protoplasts. This expression ranged from 20‐ to 123‐fold higher than the luciferase activity obtained from transfection with a luc gene mRNA. Replication of RNA beta and its derivatives i.e. ‘minus’ strand synthesis, was confirmed by Northern analysis, indicating that the high level of luc gene expression using RNA beta 2‐luc resulted from RNA amplification. ORFa of RNA beta, encoding the coat protein, was also replaced by the luc gene to yield RNA beta 1‐luc. Although transfection of RNA beta 1‐luc alone produces luciferase efficiently, neither ‘minus’ strand synthesis nor further increase of luciferase activity was observed in the presence of RNAs alpha and gamma, or RNAs alpha, beta and gamma, suggesting that the deleted sequences within ORFa are cis‐acting for replication of RNA beta. Our results demonstrate that a foreign gene can be expressed by replacement of a viral non‐structural protein gene that is essential for virus multiplication in plants, leading to a potential strategy for virus ‘containment’ with use of ‘disarmed’ plant viral vectors.

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Identification of barley stripe mosaic virus genes involved in viral RNA replication and systemic movement.

et al. 1990

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Barley stripe mosaic hordeivirus (BSMV) has a tripartite positive‐sense RNA genome which encodes seven major polypeptides. Infectious in vitro transcripts derived from full‐length wild‐type and mutant cDNA clones have been used to investigate the contribution made by various BSMV gene products to viral RNA replication and systemic movement. We show that whereas all three of the BSMV RNA components are required for plant infection, RNAs alpha and gamma can replicate together in barley protoplasts, and therefore RNA beta must encode functions required for systemic invasion of plants. The alpha a and gamma a proteins, which contain helicase and RNA polymerase sequence motifs, together comprise the essential virus‐encoded components of BSMV RNA replicase. A second BSMV protein (beta b) which contains a helicase motif is not required for RNA replication. A small cysteine‐rich protein (gamma b) is dispensable for infection of plants, but in its absence the accumulation of viral coat (beta a) and beta b proteins is significantly reduced. In addition, mutations in both the gamma b and gamma a (replicase) proteins can affect the systemic movement phenotype.

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The complete nucleotide sequence of RNAβ from the type strain of barley stripe mosaic virus

Cited by 68 publications

References 23 publications

Poa semilatent virus, a hordeivirus having no internal polydisperse poly(A) in the 3' non-coding region of the RNA genome

Poa semilatent virus, a hordeivirus having no internal polydisperse poly(A) in the 3' non-coding region of the RNA genome

BSMV genome mediated expression of a foreign gene in dicot and monocot plant cells.

Identification of barley stripe mosaic virus genes involved in viral RNA replication and systemic movement.

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