The complete nucleotide sequence of a mild strain of Bean yellow dwarf virus

Halley-Stott, Richard P.; Tanzer, Fiona L.; Martin, Darren P.; Rybicki, Edward P.

doi:10.1007/s00705-006-0933-6

Cited by 24 publications

(16 citation statements)

References 14 publications

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“…The majority of mastreviruses infect monocot plants and are transmitted by leafhoppers (33)(34)(35). The genome of mastreviruses comprises four open reading frames (ORFs).…”

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confidence: 99%

Association of Satellites with a Mastrevirus in Natural Infection: Complexity of Wheat Dwarf India Virus Disease

Kumar

Singh

et al. 2014

J Virol

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In contrast to begomoviruses, mastreviruses have not previously been shown to interact with satellites. This study reports the first identification of the association of satellites with a mastrevirus in field-grown plants. Two alphasatellite species were detected in different field samples of wheat infected with Wheat dwarf India virus (WDIV), a Cotton leaf curl Multan alphasatellite (CLCuMA) and a Guar leaf curl alphasatellite (GLCuA). In addition to the alphasatellites, a betasatellite, Ageratum yellow leaf curl betasatellite (AYLCB), was also identified in the wheat samples. No begomovirus was detected in the wheat samples, thus establishing association of the above-named satellites with WDIV. Agrobacterium-mediated inoculation of WDIV in wheat, in the presence of either of the alphasatellites or the betasatellite, resulted in infections inducing more severe symptoms. WDIV efficiently maintained each of the alphasatellites and the betasatellite in wheat. The satellites enhanced the level of WDIV DNA in wheat. Inoculation of the satellites isolated from wheat with various begomoviruses into Nicotiana tabacum demonstrated that these remain capable of interacting with the viruses with which they were first identified. Virus-specific small RNAs accumulated in wheat upon infection with WDIV but were lower in abundance in plants coinfected with the satellites, suggesting that both the alphasatellites and the betasatellite suppress RNA silencing. These results suggest that the selective advantage for the maintenance of the alphasatellites and the betasatellite by WDIV in the field is in overcoming RNA silencing-mediated host defense. IMPORTANCE Wheat is the most widely cultivated cereal crop in the world. A number of viruses are important pathogens of wheat, including the viruses of the genus

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“…The majority of mastreviruses infect monocot plants and are transmitted by leafhoppers (33)(34)(35). The genome of mastreviruses comprises four open reading frames (ORFs).…”

mentioning

confidence: 99%

Association of Satellites with a Mastrevirus in Natural Infection: Complexity of Wheat Dwarf India Virus Disease

Kumar

Singh

et al. 2014

J Virol

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“…Interestingly a mastrevirus, Bean yellow dwarf virus (BeYDV), was also identified in CSD-affected chickpeas in Pakistan. Prior to this BeYDV had only been identified affecting beans in South Africa [10,11]. In Australia, the country where the first dicot-infecting mastrevirus (TbYDV) was identified [12], a CSD-like disease of chickpea has recently been shown to be caused by three distinct viruses, referred to as Chickpea chlorosis virus-A (CpCV-A), Chickpea chlorosis virus-B (CpCV-B) and Chickpea red leaf virus (CpRLV) [13,14].…”

Section: Introductionmentioning

confidence: 99%

Analysis of the sequence of a dicot-infecting mastrevirus (family Geminiviridae) originating from Syria

et al. 2011

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Chickpea stunt disease (CSD) across southern Asia, the Middle East and North Africa is caused by a number of viruses that include single-stranded DNA viruses of the genus Mastrevirus (family Geminiviridae). Despite the importance of CSD in reducing chickpea and lentil production, until recently little was known of the nature of the pathogens causing the disease. Sequence characterisation of virus isolates from Sudan and Pakistan showed the viruses concerned to potentially be new mastrevirus species related to Bean yellow dwarf virus (BeYDV), a virus known to occur in both southern Africa and southern Asia. Here we have determined the complete nucleotide sequence of a mastrevirus associated with CSD in Syria. This virus represents a proposed new species, closely related to the recently characterised Chickpea chlorotic dwarf Sudan virus and Chickpea chlorotic dwarf Pakistan virus but with the highest sequence identity to BeYDV, for which we propose the name Chickpea chlorotic dwarf Syria virus. In addition the biological integrity of the clone was confirmed by infection of Nicotiana benthamiana plants using Agrobacterium-mediated inoculation.

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“…We chose to deconstruct the mild strain of the BeYDV (Halley-Stott et al, 2007) because it had previously been used to express heterologous proteins in plant cells (Regnard et al, 2010). BeYDV replication requires three viral elements: the cis-acting LIR, the short intergenic region (SIR), and the trans-acting replicationinitiation protein (Rep) and RepA.…”

Section: Deconstructed Viruses For the Delivery Of Sequence-specific mentioning

confidence: 99%

“…LIR and SIR sequences were derived from the mild BeYDV strain (Halley-Stott et al, 2007) (GenBank accession number DQ458791). The boundaries of the LIR and SIR sequences were defined following previously described methods (Mor et al, 2003).…”

Section: Vector Constructionmentioning

confidence: 99%

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DNA Replicons for Plant Genome Engineering

et al. 2014

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Sequence-specific nucleases enable facile editing of higher eukaryotic genomic DNA; however, targeted modification of plant genomes remains challenging due to ineffective methods for delivering reagents for genome engineering to plant cells. Here, we use geminivirus-based replicons for transient expression of sequence-specific nucleases (zinc-finger nucleases, transcription activatorlike effector nucleases, and the clustered, regularly interspaced, short palindromic repeat/Cas system) and delivery of DNA repair templates. In tobacco (Nicotiana tabacum), replicons based on the bean yellow dwarf virus enhanced gene targeting frequencies one to two orders of magnitude over conventional Agrobacterium tumefaciens T-DNA. In addition to the nuclease-mediated DNA doublestrand breaks, gene targeting was promoted by replication of the repair template and pleiotropic activity of the geminivirus replication initiator proteins. We demonstrate the feasibility of using geminivirus replicons to generate plants with a desired DNA sequence modification. By adopting a general plant transformation method, plantlets with a desired DNA change were regenerated in <6 weeks. These results, in addition to the large host range of geminiviruses, advocate the use of replicons for plant genome engineering.

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The complete nucleotide sequence of a mild strain of Bean yellow dwarf virus

Cited by 24 publications

References 14 publications

Association of Satellites with a Mastrevirus in Natural Infection: Complexity of Wheat Dwarf India Virus Disease

Association of Satellites with a Mastrevirus in Natural Infection: Complexity of Wheat Dwarf India Virus Disease

Analysis of the sequence of a dicot-infecting mastrevirus (family Geminiviridae) originating from Syria

DNA Replicons for Plant Genome Engineering

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