The Complete Nucleotide Sequence of Coxsackievirus A21

Hughes, Pamela J.; North, Christine; Minor, Philip D.; Stanway, Glyn

doi:10.1099/0022-1317-70-11-2943

Cited by 99 publications

(54 citation statements)

References 38 publications

(38 reference statements)

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“…There is no experimental evidence that recombinants between PV and NPEV are viable. However, there are findings suggesting that such recombinants circulate in nature, as the remarkable similarity to PV of the 3Ј end of the CA21 or the CA24 reference strains shows (15,36). Thus, it is clear that OPV-derived PVs can acquire highly modified genomes not only by mutation but also by genetic exchanges with vaccine or wild PV, or even with NPEV.…”

Section: Discussionmentioning

confidence: 99%

Natural Genetic Exchanges between Vaccine and Wild Poliovirus Strains in Humans

Guillot

Caro

Cuervo

et al. 2000

J Virol

182

165

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In a previous study of poliovirus vaccine-derived strains isolated from patients with vaccine-associated paralytic poliomyelitis (VAPP) (9, 11), we reported that a high proportion (over 50%) of viruses had a recombinant genome. Most were intertypic vaccine/vaccine recombinants. However, some had restriction fragment length polymorphism (RFLP) profiles different from those of poliovirus vaccine strains. We demonstrate here that five such recombinants, of 88 VAPP strains examined, carried sequences of wild (nonvaccine) origin. To identify the parental wild donor of these sequences, we used RFLP profiles and nucleotide sequencing to look for similarity in the 3D polymerase-coding region of 61 wild, cocirculating poliovirus isolates (43 type 1, 16 type 2, and 2 type 3 isolates). In only one case was the donor identified, and it was a wild type 1 poliovirus. For the other four vaccine/wild recombinants, the wild parent could not be identified. The possibility that the wild sequences were of a non-poliovirus-enterovirus origin could not be excluded. Another vaccine/wild recombinant, isolated in Belarus from a VAPP case, indicated that the poliovirus vaccine/wild recombination is not an isolated phenomenon. We also found wild polioviruses (2 of 15) carrying vaccine-derived sequences in the 3 moiety of their genome. All these results suggest that genetic exchanges with wild poliovirus and perhaps with nonpoliovirus enteroviruses, are also a natural means of evolution for poliovirus vaccine strains.

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Section: Discussionmentioning

confidence: 99%

Natural Genetic Exchanges between Vaccine and Wild Poliovirus Strains in Humans

Guillot

Caro

Cuervo

et al. 2000

J Virol

182

165

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“…Biotinylated nested-PCR products were sequenced using a direct solid-phase T7-polymerase-mediated nucleotide sequencing strategy, using either a fluorescein-labelled sequencing primer for analysis with an ALF automated DNA sequencer (Pharmacia) as described previously by Nicholson et al (1995), or an unlabelled sequencing primer and Dyedeoxy terminators (Applied Biosystems) for analysis with a 373 automated DNA sequencer (Applied Biosystems). Sequences thus derived were compared with published enterovirus sequences (Chang et al, 1989;Drebot et al, 1994;Hughes et al, 1989;Dahllund e~ al., 1995;Iizuka et al, 1987;Jenkins et al, 1987;Klump et al, 1990;Kraus et al, 1995;Lindberg et al, 1987;Pulli et al, 1995;P6yry et al, 1994;Romero & Rotbart, 1995;Ryan et al, 1990;Supanaranond et al, 1992;Toyoda et al, 1984;Zhang et at., 1993;Zheng et al, 1995), using DNASIS to estabish the likely serotypic identity of viruses detected by PCR.…”

Section: Methodsmentioning

confidence: 99%

Enterovirus infection of the central nervous system of humans: lack of association with chronic neurological disease

Muir

Nicholson

Spencer

et al. 1996

Journal of General Virology

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“…2A) and were very similar to the corresponding sequences of polioviruses (equal or more than 97"9 %). The non-polio enteroviruses most similar to polioviruses in the nonstructural protein-coding region are the coxsackieviruses A21 (CA21) and A24 (CA24) (Hughes et al, 1989;Supanaranond et al, 1992). We compared the amino acid sequence of the 2-Is strain to those of CA21 and CA24 in a region where the coxsackieviruses differ from polioviruses: the C-terminal part of the 2C protein (amino acids 173-268) (Fig.…”

Section: Hpalimentioning

confidence: 99%

Tripartite genome organization of a natural type 2 vaccine/nonvaccine recombinant poliovirus

Georgescu

Delpeyroux²,

Crainic³

1995

Journal of General Virology

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Intertypic vaccine/vaccine recombinant polioviruses are frequently isolated from vaccine-associated paralytic poliomyelitis cases (VAPP). We identified a vaccine/ nonvaccine poliovirus recombinant as the causative agent of a lethal VAPP. Partial RNA sequencing revealed a tripartite recombinant structure of the viral genome. This consisted of a central capsid core of vaccine origin flanked by two units of nonvaccine origin. The first nonvaccine genomic unit spanned the whole 5' noncoding region, and the second one almost the entire nonstructural protein-coding region and the 3' noncoding region. Amino acid and nucleotide sequence similarities in the 3' and 5' unidentified regions indicated that the viral donor(s) were poliovirus species, suggesting recombination between a vaccine-derived and a wild poliovirus. The nonvaceine donor(s) could not be identified among the investigated wild polioviruses cocirculating in the same geographical area. This is the first report of a natural recombination event occurring in the 5' genomic extremity of poliovirus. The neurovirulence for transgenic mice and the pathogenicity for humans of the recombinant suggested that the modular genomic organization of this virus might have conferred a selective advantage over its vaccine parent.

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The Complete Nucleotide Sequence of Coxsackievirus A21

Cited by 99 publications

References 38 publications

Natural Genetic Exchanges between Vaccine and Wild Poliovirus Strains in Humans

Natural Genetic Exchanges between Vaccine and Wild Poliovirus Strains in Humans

Enterovirus infection of the central nervous system of humans: lack of association with chronic neurological disease

Tripartite genome organization of a natural type 2 vaccine/nonvaccine recombinant poliovirus

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