The complete mitochondrial genome sequence and gene organization of Tridentiger trigonocephalus (Gobiidae: Gobionellinae) with phylogenetic consideration

Abstract

The complete mitochondrial genome plays an important role in studies of genome-level characteristics and phylogenetic relationships. Here we determined the complete mitogenome sequence of Tridentiger trigonocephalus (Perciformes, Gobiidae), and discovered its phylogenetic relationship. This circular genome was 16 662 bp in length, and consisted of 37 typical genes, including 13 protein-coding genes, 22 tRNA genes, and two rRNA genes. The gene order of T. trigonocephalus mitochondrial genome was identical to those observed in most other vertebrates. Of 37 genes, 28 were encoded by heavy strand, while the others were encoded by light strand. The phylogenetic tree constructed by 13 concatenated protein-coding genes showed that T. trigonocephalus was closest to T. bifasciatus, and then to T. barbatus among the 20 species within suborder Gobioidei. This work should facilitate the studies on population genetic diversity, and molecular evolution in Gobioidei fishes.

<span>Molecular-genetic identification of chameleon goby Tridentiger trigonocephalus (Gill, 1859) in the Black Sea</span>

“…This clearly differed from a small selection of chameleon gobies from their native range (the Sea of Japan and the East China Sea) (see Table 3). Selection was small because in NCBI in total 3 haplotypes were deposited for the 16S rRNA gene of this species (Mukai et al 1997;Wei et al 2016;Huang et al 2016).…”

“…At the same time, the extent of scientific knowledge of the genetic variability of T. trigonocephalus in its newly settled range is obviously insufficient. So far only the mitochondrial and whole-genome sequences (Wei et al 2016), separate genes of mtDNA: СOI, 12S, 16S, cyt b (Mukai et al 1997;Miya et al 2015;Bingpeng et al 2018), and also nuclear gene -RyR3 (Gu & Chen 2013) have been studied, usually for the purposes of taxonomic identification and to some extent phylogeny. These studies dealt with areas of the natural range of the chameleon goby (Japanese Islands, Taiwan, coast of mainland China in the area of city of Fuzhou and the mouth of the Yangtze River).…”