The complete mitochondrial genome of the marine polychaete:Perinereis aibuhitensis(Phyllodocida, Nereididae)

Abstract

We determined the complete mitochondrial genome sequence of the marine polychaete Perinereis aibuhitensis (Grube, 1878) (Phyllodocida, Nereididae), a dominant species in the mudflat of the West Pacific and Indian Ocean. The complete genome of P. aibuhitensis is 15,852 bp in size including 13 protein coding genes (PCGs), 2 rRNA and 22 tRNA with the same gene order and structure as those of other Nereididae species. The nucleotide composition is 29.5% A, 21.2% C, 14.1% G, 35.2% T, showing a high content of A+T with G being used least in the third codon position (6.7%). All PCGs use ATG as the start codon while for the stop codon COI and ND1 use incomplete codon of T. The mitogenome sequence of P. aibuhitensis is second to that of P. nuntia known in the genus Perinereis, which will provide useful information for understanding evolutionary history of the genus Perinereis within the family Nereididae.

The phylogeny of Nereididae (Annelida) based on mitochondrial genomes

“…1) to 17,469 bp in Platynereis massiliensis . All genes were coded on the same strand, consistent with previously published Nereididae mitochondrial genomes (Boore & Brown, ; Chen, Li, et al, ; Kim, Choi, Kim, & Min, ; Kim et al, ; Kim, Kim, & Lee, ; Lin et al, , ; Park et al, , ; Seixas et al, ; Won et al, ). Appendix shows information on base composition and nucleotide bias in the mitochondrial genomes sequenced.…”

“…As of 2019, 11 complete Nereididae mitochondrial genomes had been published (Boore & Brown, ; Chen, Li, et al, ; Kim, Kim, & Lee, , ; Kim et al, ; Lin, Audira, & Hsiao, ; Lin, Shen, & Hsiao, ; Park, Lee, & Kim, ; Park, Lee, Shin, & Kim, ; Seixas, Paiva, & Russo, ; Won, Rhee, Shin, & Lee, ) and two distinct tRNA gene order arrangements have been recognized by Park et al () that vary in the position of the ATP8 gene and three tRNAs. These arrangements have been referred to as ‘Group 1’ with tRNA‐Tyr, ATP8, tRNA‐Met and tRNA‐Asp and ‘Group 2’ with tRNA‐Met, tRNA‐Asp, ATP8 and tRNA‐Tyr.…”

The complete mitochondrial genome of the marine polychaete: Hediste diadroma (Phyllodocida, Nereididae)

“…The genomic DNA was extracted from the muscle tissue and the mitogenome sequences were analyzed in two ways: First, dozens of mitochondrial DNA fragments were amplified through PCR with primer sets designed from the partial sequences of COI, COIII, CytB, ND4 and 16S genes of H. diadroma, and sequenced by the Sanger method, which resulted in a sequence of 12,578bp; Then, the gap sequence of 3178bp was filled up by application of Illumina Hiseq2000 sequencing platform. The sequences were assembled and annotated in comparison with the previously reported mitogenome sequences of Nereididae species (Boore 2001;Kim et al 2015) usingGeneious v9.1.2 (Biomatters Ltd. Auckland, New Zealand; Kearse et al 2012).…”

Determination of complete mitogenome sequence for Eastern Asian population of Cheilonereis cyclurus (Harrington, 1897) (Polychaeta: Nereididae)

“…Also, the comparison of partial mitochondrial COI sequences revealed significant difference between east and west north Pacific populations ( Figure 1(A); personal data of the author). Although, the comparison of complete mitogenome sequence may provide more valuable evolutionary information for phylogenetic studies (Chen et al 2016), only seven nereidids are available (Boore 2001;Won et al 2013;Kim et al 2014;Chen et al 2016;Kim et al 2016;Lin et al 2016;Park et al 2016). The purpose of this study was to determine the complete mitogenome sequence for eastern Asian population of C. cyclurus.…”