The complete mitochondrial genome of the oriental fruit moth Grapholita molesta (Busck) (Lepidoptera: Tortricidae)

Gong, Ya‐Jun; Bao-cai, Shi; Kang, Zong-Jiang; Zhang, Fan; Wei, Shu‐Jun

doi:10.1007/s11033-011-1049-y

Cited by 62 publications

(42 citation statements)

References 41 publications

(52 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…suppressalis are different in length and structure from those in Alloeorhynchus bakeri (Hemiptera) and M . sexta , but similar to Grapholita molesta , Liriomyza trifolii (Diptera) and Libelloides macaronius (Neuroptera) 22, 31, 66-68. Nucleotide variability among domains and helices is unevenly distributed in the secondary structures of the rrnL and rrnS genes.…”

Section: Discussionmentioning

confidence: 91%

Characterization of the Complete Mitochondrial Genomes of Cnaphalocrocis medinalis and Chilo suppressalis (Lepidoptera: Pyralidae)

Chai¹,

Du²,

Zhai³

2012

Int. J. Biol. Sci.

View full text Add to dashboard Cite

The complete mitochondrial genomes (mitogenomes) of Cnaphalocrocis medinalis and Chilo suppressalis (Lepidoptera: Pyralidae) were determined and analyzed. The circular genomes were 15,388 bp long for C. medinalis and 15,395 bp long for C. suppressalis. Both mitogenomes contained 37 genes, with gene order similar to that of other lepidopterans. Notably, 12 protein-coding genes (PCGs) utilized the standard ATN, but the cox1 gene used CGA as the initiation codon; the cox1, cox2, and nad4 genes in the two mitogenomes had the truncated termination codons T, T, and TA, respectively, but the nad5 gene was found to use T as the termination codon only in the C. medinalis mitogenome. Additionally, the codon distribution and Relative Synonymous Codon Usage of the 13 PCGs in the C. medinalis mitogenome were very different from those in other pyralid moth mitogenomes. Most of the tRNA genes had typical cloverleaf secondary structures. However, the dihydrouridine (DHU) arm of the trnS1(AGN) gene did not form a stable stem-loop structure. Forty-nine helices in six domains, and 33 helices in three domains were present in the secondary structures of the rrnL and rrnS genes of the two mitogenomes, respectively. There were four major intergenic spacers, except for the A+T-rich region, spanning at least 12 bp in the two mitogenomes. The A+T-rich region contained an 'ATAGT(A)'-like motif followed by a poly-T stretch in the two mitogenomes. In addition, there were a potential stem-loop structure, a duplicated 25-bp repeat element, and a microsatellite '(TA)13' observed in the A+T-rich region of the C. medinalis mitogenome. A poly-T motif, a duplicated 31-bp repeat element, and a 19-bp triplication were found in the C. suppressalis mitogenome. However, there are many differences in the A+T-rich regions between the C. suppressalis mitogenome sequence in the present study and previous reports. Finally, the phylogenetic relationships of these insects were reconstructed based on amino acid sequences of mitochondrial 13 PCGs using Bayesian inference and maximum likelihood methods. These molecular-based phylogenies support the traditional morphologically based view of relationships within the Pyralidae.

show abstract

Section: Discussionmentioning

confidence: 91%

Characterization of the Complete Mitochondrial Genomes of Cnaphalocrocis medinalis and Chilo suppressalis (Lepidoptera: Pyralidae)

Chai¹,

Du²,

Zhai³

2012

Int. J. Biol. Sci.

View full text Add to dashboard Cite

show abstract

“…Most of the seven gene sequences have been previously shown to be powerful markers in population genetic studies (Meng et al 2008;Ma et al 2012). The amplification and sequencing primers were designed using PRIMER PREMIER software version 5 according to the complete OFM mitochondrial genome (Gong et al 2012) (Table S2, Supporting information). Polymerase chain reaction (PCR) was conducted using the Mastercycler pro system (Eppendorf, Germany) under the following conditions: an initial denaturation for 3 min at 94°C, followed by 35 cycles of 10 s at 94°C, 15 s at 48°C and 2 min for the region of cox1-atp6 and nad5 and 1 min for the regions of nad1 and rrnL at 68°C, and a subsequent final extension for 10 min at 68°C.…”

Section: Dna Extraction Mitochondrial Gene Sequencing and Microsatelmentioning

confidence: 99%

Population genetic structure and approximate Bayesian computation analyses reveal the southern origin and northward dispersal of the oriental fruit moth Grapholita molesta (Lepidoptera: Tortricidae) in its native range

et al. 2015

Self Cite

View full text Add to dashboard Cite

Population genetic structure and approximate Bayesian computation analyses reveal the southern origin and northward dispersal of the oriental fruit moth Grapholita molesta (Lepidoptera: Tortricidae) in its native range AbstractThe oriental fruit moth (OFM) Grapholita molesta is one of the most destructive orchard pests. Assumed to be native to China, the moth is now distributed throughout the world. However, the evolutionary history of this moth in its native range remains unknown. In this study, we explored the population genetic structure, dispersal routes and demographic history of the OFM in China and South Korea based on mitochondrial genes and microsatellite loci. The Mantel test indicated a significant correlation between genetic distance and geographical distance in the populations. Bayesian analysis of population genetic structure (BAPS) identified four nested clusters, while the GENELAND analysis inferred five genetic groups with spatial discontinuities. Based on the approximate Bayesian computation approach, we found that the OFM was originated from southern China near the Shilin area of Yunnan Province. The early divergence and dispersal of this moth was dated to the Penultimate glaciation of Pleistocene. Further dispersal from southern to northern region of China occurred before the last glacial maximum, while the expansion of population size in the derived populations in northern region of China occurred after the last glacial maximum. Our results indicated that the current distribution and structure of the OFM were complicatedly influenced by climatic and geological events and human activities of cultivation and wide dissemination of peach in ancient China. We provide an example on revealing the origin and dispersal history of an agricultural pest insect in its native range as well as the underlying factors.

show abstract

“…The stem regions of H1057/D18, H1087/D19 and H991/D17 in domain II are difficult to fold under the criteria of Watson-Crick pairs, and thus different from M. sexta with regard to a large internal loop. The stem H2347/G13 is also highly variable within the order Lepidoptera with regard to sequence variation and the (AT) n insertion, such as (AT) 14 in M. sexta and (AT) 12 in Grapholitha molesta (Gong et al, 2012). While some bases indentified as highly conserved region, such as the stems H2064/G2, H2077/G3, H2246/G6, H2259/G7, H2282, and their correspondingly large loops are found adjacent to and even within the hypervariable region, these conserved sites may serve as anchor points for the secondary structure of the hypervariable region (Hao et al, 2007).…”

Section: Ribosomal and Transfer Rna Genesmentioning

confidence: 99%

Complete sequence of the mitochondrial genome of the Japanese buff-tip moth, Phalera flavescens (Lepidoptera: Notodontidae)

Sun¹,

Sun²,

Wang³

et al. 2012

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. We sequenced the complete mitochondrial genome of Phalera flavescens. The mitogenome is 15,659 bp in length, including 13 protein-coding genes (atp6, atp8, cox1-3, nad1-6, nad4L, cob), two ribosomal RNAs (rrnS and rrnL), 22 transfer RNAs and an AT-rich region, a putative control region (D-loop). Gene order and orientation were found to be identical to those of other completely sequenced lepidopteran mitogenomes. All 13 protein-coding genes start with the common codon ATN, except for the cox1 gene, which uses CGA as the initial codon. Nine of the 13 protein-coding genes stop with codon TAA, while the cox1, cox2, nad5, and nad4 genes stop with the single nucleotide T. All tRNA genes can be folded into canonical cloverleaf secondary structure, except for trnS1, which loses the ''DHU'' arm. Six overlapping sequences totaling 20 bp (1-8 bp for each sequence) and 16 intergenic spacer sequences, totaling 276 bp (1-58 bp for each sequence) are scattered throughout the genome; the largest intergenic spacer is located between the trnQ and nad2 genes. A microsatellitelike structure (AT) 6 ACC(AT) 6 and 16-bp poly-T elements preceded by the ATTTA motif are present in the D-loop region. Additionally, unexpectedly, an extra 190-bp insertion, with unknown function, was found in the small subunit rRNA gene (rrnS); this gene is the longest known (1020 bp) among all of the Lepidoptera.

show abstract

The complete mitochondrial genome of the oriental fruit moth Grapholita molesta (Busck) (Lepidoptera: Tortricidae)

Cited by 62 publications

References 41 publications

Characterization of the Complete Mitochondrial Genomes of Cnaphalocrocis medinalis and Chilo suppressalis (Lepidoptera: Pyralidae)

Characterization of the Complete Mitochondrial Genomes of Cnaphalocrocis medinalis and Chilo suppressalis (Lepidoptera: Pyralidae)

Population genetic structure and approximate Bayesian computation analyses reveal the southern origin and northward dispersal of the oriental fruit moth Grapholita molesta (Lepidoptera: Tortricidae) in its native range

Complete sequence of the mitochondrial genome of the Japanese buff-tip moth, Phalera flavescens (Lepidoptera: Notodontidae)

Contact Info

Product

Resources

About