The complete mitochondrial genome ofArtemia sinicaCai, 1989 (Crustacea: Anostraca) using next-generation sequencing

Abstract

The complete mitochondrial genome of Artemia sinica was obtained using the next-generation sequencing (NGS) method. The mitochondrial genome is a circular molecule of 15,689 bp in length, with the typical structure of 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs) and 2 ribosomal RNA genes, and a non-coding control region (CR). The base composition is 31.53% A, 18.99% C, 16.50% G, and 32.98% T, with an A þ T content of 64.51%. All tRNAs have a cloverleaf structure excepting tRNA-Ser 1 , that represents the D-loop structure.

Validation of tWO novel primers for THE promising amplification of the mitogenomic Cytochrome c Oxidase subunit I (COI) barcoding region in Artemia aff. sinica (Branchiopoda, Anostraca)

“…Due to the lack of unequivocal morphological characters, there is no classic identification key for Artemia species (Asem et al, 2010-2020). Therefore, molecular phylogenetic methods have been employed to delimit the taxonomic status of Artemia populations (Munoz et al, 2008; Maccari et al, 2013; Eimanifar et al, 2014, 2020; Asem et al, 2016b-2019-2021b; Saji et al, 2019; Shen et al, 2021). Hence, it is necessary to provide a dataset of a large number of sequences from each population, to reliably analyse population genetic and phylogenetic relationships among them (Maccari et al, 2013).…”

“…The complete mitochondrial genome of Artemia sinica (MK069595) was chosen as a reference sequence (Asem et al, 2019). The online primer designing tool of NCBI was employed to design the desired primers (NCBI, 2021(NCBI, -2022.…”

“…According to the gene order of the Artemia mitogenome, tRNA Tyr is placed at the rear of COI. Because tRNA Tyr is located on the L-strand (Asem et al, 2019(Asem et al, , 2021a, its reverse sequence on the H-strand was followed to design the F-primer. In practice this meant, that the last 13 bp of the reverse sequence of tRNA Tyr on the H-strand (1347-1359 sites) and 7 bp of the beginning of COI (1361-1367 sites) were chosen to design the F-primer.…”

The complete mitochondrial genome ofArtemia persimilisPiccinelli and Prosdocimi, 1968 (Crustacea: Anostraca)

“… 2013 ; Asem et al. 2019 ). But for another bisexual species Artemia persimilis Piccinelli and Prosdocimi, 1968, a species endemic to the South American and geographically restricted to Argentina and Chile (Sainz Escudero et al.…”

The complete mitochondrial genome ofArtemia salinaLeach, 1819 (Crustacea: Anostraca)

“… 2013 ; Asem et al. 2019 ). As for Artemia salina , a bisexual species endemic to the Mediterranean Basinare, is being threatened by habitat loss and the ongoing A. franciscana invasion (Muñoz et al.…”