The complete mitochondrial genome of <i>Oestrus ovis</i> (Linnaeus, 1758) (Diptera: Oestridae)

Aleix-Mata, Gaël; Pérez, Jesús M.; Sánchez, Antonio

doi:10.1080/23802359.2021.1934174

Cited by 7 publications

(1 citation statement)

References 11 publications

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“…In the present study, the positions of Oestrus ovis and Rhinoestrus usbekistanicus in the phylogenetic tree (Fig. 5) are supporting the ndings of the earlier report [41], where in the said two Oestrinae species could be sister groups as per the phylogenetic tree analysis involving the whole mitochondrial genome of the 13 Oestridae species.…”

Section: Resultssupporting

confidence: 91%

First report on Molecular Characterization of Oesturs ovis in sheep from India

Nagarajan

Kanagarajadurai

Pachaiyappan

et al. 2023

Preprint

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Purpose Upon post mortem examination of sheep (from SRRC, Mannavanur, South India) having the history of natural death, the nasal sinuses of the animals contained dipteran larvae. The objective of the present study is to characterize the dipteran larvae species infesting the sheep being maintained at SRRC, Mannavanur, by means of COI gene based PCR. Methods During the last week of May 2021, an Avikalin male sheep (20 months old) died suddenly. Upon Post mortem examination, the skull of the dead sheep was having of larvae in the nasal sinuses. The larvae were washed in PBS (pH 7.2) and preserved in 70 % alcohol. The larvae taken out of 70 % alcohol were thoroughly ground to make a fine powder using liquid Nitrogen in a sterile mortar and pestle. The maggot in powder form was then used for the isolation of total genomic DNA isolation. Using the isolated genomic DNA from the larvae as a template, Cytochrome c oxidase subunit I (COI) gene based PCR was employed using the primers designed based on the COI gene of reference isolate of Oestrus ovis available in the GenBank. Full length COI gene (1534 bp) gene of Oestrus ovis in sheep from South India was targeted in the PCR experiment. The pTZ57R/T vector was used for the cloning of the PCR amplified fragment and the confirmed recombinant plasmid was subjected to sequencing experiments. The resultant nucleotide sequences of COI gene of the O.ovis in sheep from South India was analysed with that of 29 dipteran species from different different geographical areas of the world (available in GenBank) by using standard bioinformatics tools. Results In addition to morphological examination, based on COI gene based PCR, eventual sequencing experiments and BLAST analysis, it was confirmed that the larvae in the nasal sinuses of sheep from South India were Oestrus ovis. The South Indian isolate of Oestrus ovis is sharing 100% sequence identity both at nucleotide and amino acid levels with that of O.ovis from Spain. The North Indian isolate of O.ovis (from Jammu) exhibited 92 & and 99 % identity at respective nucleotide and amino acid levels with South Indian isolate. With other members of the subfamily Oestrinae, the share of per cent nucleotide and amino acid identities of South Indian O.ovis ranged from 85-86 % and 95-96 %, respectively. O.ovis from South India was grouped with the other members of Oestrinae from different geographical areas of the globe in the analysis of phylogenetic tree based on COI amino acid sequences. ConclusionBased on the research findings, it is concluded that Oestrus ovis is the dipteran species infesting the sheep at Mannavanur, Tamil Nadu, India. To our knowledge, this is the first report on full length nucleotide sequences of COI gene of O.ovis in sheep from Indian subcontinent. An investigation on the report of human ocular infections and the accidental hosts for O ovis in the area of the present study has to be explored.

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Section: Resultssupporting

confidence: 91%

First report on Molecular Characterization of Oesturs ovis in sheep from India

Nagarajan

Kanagarajadurai

Pachaiyappan

et al. 2023

Preprint

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Prevalence of nasopharyngeal myiasis in roe deer (Capreolus capreolus) from an area with high sympatry between wild and domestic ungulates in Central Spain

Martínez-Calabuig,

Panadero,

Varas

et al. 2024

Eur J Wildl Res

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Infestation by the sheep bot fly Oestrus ovis was firstly reported in a single roe deer from Central Spain in 2022. For assessing the current situation of nasal myiases in this ungulate in this area, the nasopharyngeal cavities of 184 roe deer hunted in Central Spain between January-June 2023 were examined. All larvae were recovered and morphologically identified; in addition, species identification was molecularly confirmed in a subset of specimens. Forty-four roe deer (23.9%; CI 95 17.95–30.74) were positive for different Oestrinae larval stages. Twenty-six animals (14.1%; CI 95 9.44–20.02%) were infested by the roe deer nasal bot fly (Cephenemyia stimulator) with a mean intensity of 35.2 (SD 49.71) larvae/infested animal, and eighteen (9.8%; CI 95 5.90-15.02%) roe deer harboured the sheep bot fly (O. ovis), with a mean intensity of 2.0 (SD 1.33) larvae/infested animal. No mixed infestations by both Oestrinae were found in a single animal. All larval instars (L1, L2 and L3) of both species were identified. Most C. stimulator specimens were located at the nasal turbinates, and a small percentage (3.2%) at the pharynx; all O. ovis larvae were found at the nasal turbinates. Since O. ovis is highly prevalent in sheep and goat flocks from Central Spain, the high sympatry between roe deer and small ruminant populations in the studied area may have increased the risk of cross-infection. Moreover, the finding of mature L3 of O. ovis suggests that this species can complete its life cycle in roe deer. Therefore, monitoring bot flies in sheep and goat flocks as well as in sympatric wild ruminants is strongly recommended for achieving an optimum control of nasal myiases.

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